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Strain Name:
C57BL/6J-MtgxR9712Btlr/Mmmh
Stock Number:
069959-MU
Citation ID:
RRID:MMRRC_069959-MU
Other Names:
R9712 (G1)
Major Collection:

Strain Information

Slc12a6
Name: solute carrier family 12, member 6
Synonyms: KCC3, gaxp
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 107723
Homologene: 21069
Atp1a1
Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms: Atpa-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11928
HGNC: HGNC:799
Homologene: 564
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: AS3, Aprin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100710
Homologene: 41001
Adsl
Name: adenylosuccinate lyase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11564
HGNC: HGNC:291
Homologene: 12
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to A, chromosome 1 at 5,598,873 bp (GRCm38)
  • T to C, chromosome 1 at 24,328,067 bp (GRCm38)
  • T to C, chromosome 1 at 39,385,232 bp (GRCm38)
  • A to T, chromosome 1 at 53,559,140 bp (GRCm38)
  • T to C, chromosome 1 at 93,686,507 bp (GRCm38)
  • T to C, chromosome 1 at 128,405,462 bp (GRCm38)
  • T to C, chromosome 1 at 165,513,112 bp (GRCm38)
  • T to C, chromosome 1 at 173,749,358 bp (GRCm38)
  • T to A, chromosome 1 at 175,964,006 bp (GRCm38)
  • T to A, chromosome 1 at 183,135,815 bp (GRCm38)
  • G to A, chromosome 2 at 76,734,248 bp (GRCm38)
  • G to A, chromosome 2 at 86,347,239 bp (GRCm38)
  • A to T, chromosome 2 at 87,671,761 bp (GRCm38)
  • C to A, chromosome 2 at 92,376,643 bp (GRCm38)
  • C to A, chromosome 2 at 93,980,973 bp (GRCm38)
  • T to C, chromosome 2 at 112,356,472 bp (GRCm38)
  • C to T, chromosome 2 at 150,254,576 bp (GRCm38)
  • T to A, chromosome 2 at 181,718,419 bp (GRCm38)
  • T to C, chromosome 3 at 31,116,860 bp (GRCm38)
  • C to T, chromosome 3 at 62,423,613 bp (GRCm38)
  • T to C, chromosome 3 at 101,591,441 bp (GRCm38)
  • G to A, chromosome 4 at 11,961,607 bp (GRCm38)
  • T to A, chromosome 4 at 46,144,837 bp (GRCm38)
  • T to G, chromosome 4 at 53,679,972 bp (GRCm38)
  • T to A, chromosome 4 at 106,347,367 bp (GRCm38)
  • C to T, chromosome 4 at 144,528,784 bp (GRCm38)
  • T to G, chromosome 4 at 152,547,064 bp (GRCm38)
  • A to G, chromosome 5 at 107,419,781 bp (GRCm38)
  • T to A, chromosome 5 at 110,756,643 bp (GRCm38)
  • G to T, chromosome 5 at 114,633,150 bp (GRCm38)
  • T to A, chromosome 5 at 116,482,393 bp (GRCm38)
  • T to A, chromosome 5 at 120,814,485 bp (GRCm38)
  • A to T, chromosome 5 at 121,310,681 bp (GRCm38)
  • C to T, chromosome 5 at 136,309,819 bp (GRCm38)
  • T to C, chromosome 5 at 143,914,796 bp (GRCm38)
  • A to T, chromosome 5 at 150,805,663 bp (GRCm38)
  • A to G, chromosome 6 at 29,417,442 bp (GRCm38)
  • A to G, chromosome 6 at 65,456,140 bp (GRCm38)
  • G to A, chromosome 6 at 125,624,573 bp (GRCm38)
  • CTCTTCATGATTTTCTT to CTCTT, chromosome 6 at 131,301,649 bp (GRCm38)
  • T to C, chromosome 7 at 6,393,879 bp (GRCm38)
  • A to G, chromosome 7 at 66,773,630 bp (GRCm38)
  • A to T, chromosome 7 at 108,925,528 bp (GRCm38)
  • G to A, chromosome 8 at 40,795,453 bp (GRCm38)
  • A to T, chromosome 8 at 45,017,380 bp (GRCm38)
  • G to A, chromosome 8 at 91,129,727 bp (GRCm38)
  • A to G, chromosome 9 at 97,079,177 bp (GRCm38)
  • G to A, chromosome 9 at 108,986,503 bp (GRCm38)
  • C to A, chromosome 9 at 109,058,737 bp (GRCm38)
  • A to T, chromosome 9 at 119,790,010 bp (GRCm38)
  • A to T, chromosome 9 at 120,955,829 bp (GRCm38)
  • C to T, chromosome 10 at 18,168,434 bp (GRCm38)
  • A to T, chromosome 10 at 34,001,156 bp (GRCm38)
  • A to T, chromosome 11 at 48,907,389 bp (GRCm38)
  • A to G, chromosome 11 at 50,385,869 bp (GRCm38)
  • G to T, chromosome 11 at 69,100,649 bp (GRCm38)
  • T to A, chromosome 11 at 109,942,337 bp (GRCm38)
  • A to T, chromosome 12 at 11,457,592 bp (GRCm38)
  • T to C, chromosome 13 at 55,246,043 bp (GRCm38)
  • A to T, chromosome 13 at 74,192,908 bp (GRCm38)
  • C to T, chromosome 13 at 91,987,973 bp (GRCm38)
  • T to C, chromosome 13 at 93,065,373 bp (GRCm38)
  • T to A, chromosome 14 at 37,060,127 bp (GRCm38)
  • T to C, chromosome 14 at 51,988,958 bp (GRCm38)
  • T to C, chromosome 14 at 57,481,396 bp (GRCm38)
  • A to T, chromosome 14 at 101,507,410 bp (GRCm38)
  • C to T, chromosome 14 at 101,729,457 bp (GRCm38)
  • T to A, chromosome 15 at 54,459,784 bp (GRCm38)
  • A to T, chromosome 15 at 80,955,639 bp (GRCm38)
  • A to G, chromosome 15 at 83,313,533 bp (GRCm38)
  • G to C, chromosome 15 at 90,985,325 bp (GRCm38)
  • G to T, chromosome 15 at 90,995,512 bp (GRCm38)
  • C to A, chromosome 16 at 45,774,977 bp (GRCm38)
  • A to T, chromosome 16 at 65,529,872 bp (GRCm38)
  • T to C, chromosome 17 at 6,752,995 bp (GRCm38)
  • A to C, chromosome 17 at 20,958,796 bp (GRCm38)
  • A to T, chromosome 17 at 21,261,698 bp (GRCm38)
  • A to C, chromosome 17 at 32,396,562 bp (GRCm38)
  • A to T, chromosome 18 at 11,059,064 bp (GRCm38)
  • A to T, chromosome 19 at 3,715,784 bp (GRCm38)
  • AGATCTC to A, chromosome 19 at 9,007,028 bp (GRCm38)
  • GATCTCTAT to GAT, chromosome 19 at 9,007,029 bp (GRCm38)
  • A to T, chromosome 19 at 22,715,352 bp (GRCm38)
  • A to G, chromosome 19 at 30,056,481 bp (GRCm38)
  • A to G, chromosome 19 at 47,129,302 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9712 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069959-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.