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Strain Name:
C57BL/6J-MtgxR9747Btlr/Mmmh
Stock Number:
069993-MU
Citation ID:
RRID:MMRRC_069993-MU
Other Names:
R9747 (G1)
Major Collection:

Strain Information

Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Kif5a
Name: kinesin family member 5A
Synonyms: Kns, Kif5, D10Bwg0738e, Khc
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16572
VEGA: 10
HGNC: HGNC:6323
Homologene: 55861
Itga6
Name: integrin alpha 6
Synonyms: Cd49f, 5033401O05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16403
HGNC: HGNC:6142
Homologene: 20091
Gabrg1
Name: gamma-aminobutyric acid type A receptor subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Cd36
Name: CD36 molecule
Synonyms: FAT, fatty acid translocase, Scarb3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12491
HGNC: HGNC:1663
Homologene: 73871
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 20,978,704 bp (GRCm38)
  • A to T, chromosome 1 at 34,438,920 bp (GRCm38)
  • A to T, chromosome 1 at 54,259,948 bp (GRCm38)
  • A to T, chromosome 1 at 172,478,215 bp (GRCm38)
  • A to T, chromosome 2 at 24,091,092 bp (GRCm38)
  • G to T, chromosome 2 at 29,174,365 bp (GRCm38)
  • C to A, chromosome 2 at 57,999,465 bp (GRCm38)
  • A to G, chromosome 2 at 60,306,328 bp (GRCm38)
  • C to T, chromosome 2 at 71,826,527 bp (GRCm38)
  • C to T, chromosome 2 at 76,763,949 bp (GRCm38)
  • T to A, chromosome 2 at 87,150,554 bp (GRCm38)
  • C to T, chromosome 2 at 101,696,684 bp (GRCm38)
  • G to T, chromosome 2 at 157,192,046 bp (GRCm38)
  • A to G, chromosome 2 at 164,765,180 bp (GRCm38)
  • A to T, chromosome 3 at 63,954,003 bp (GRCm38)
  • A to C, chromosome 3 at 87,367,797 bp (GRCm38)
  • C to T, chromosome 3 at 90,270,521 bp (GRCm38)
  • C to T, chromosome 3 at 92,850,521 bp (GRCm38)
  • T to C, chromosome 3 at 94,937,337 bp (GRCm38)
  • A to G, chromosome 3 at 123,546,812 bp (GRCm38)
  • T to C, chromosome 3 at 126,959,018 bp (GRCm38)
  • T to A, chromosome 4 at 47,312,208 bp (GRCm38)
  • T to C, chromosome 4 at 101,158,890 bp (GRCm38)
  • C to T, chromosome 4 at 118,869,020 bp (GRCm38)
  • A to G, chromosome 4 at 131,922,048 bp (GRCm38)
  • T to A, chromosome 5 at 15,711,654 bp (GRCm38)
  • T to G, chromosome 5 at 17,814,734 bp (GRCm38)
  • T to G, chromosome 5 at 67,268,579 bp (GRCm38)
  • T to C, chromosome 5 at 70,780,686 bp (GRCm38)
  • T to C, chromosome 5 at 140,760,847 bp (GRCm38)
  • C to A, chromosome 6 at 18,285,637 bp (GRCm38)
  • T to C, chromosome 6 at 30,439,860 bp (GRCm38)
  • C to T, chromosome 6 at 56,753,840 bp (GRCm38)
  • G to T, chromosome 6 at 67,766,231 bp (GRCm38)
  • A to G, chromosome 6 at 90,644,156 bp (GRCm38)
  • A to G, chromosome 6 at 95,121,857 bp (GRCm38)
  • A to G, chromosome 6 at 126,962,014 bp (GRCm38)
  • A to T, chromosome 6 at 142,449,455 bp (GRCm38)
  • T to C, chromosome 7 at 19,941,914 bp (GRCm38)
  • C to T, chromosome 7 at 46,731,653 bp (GRCm38)
  • C to T, chromosome 7 at 47,426,710 bp (GRCm38)
  • T to A, chromosome 7 at 65,983,722 bp (GRCm38)
  • A to T, chromosome 7 at 105,763,475 bp (GRCm38)
  • T to A, chromosome 7 at 139,611,991 bp (GRCm38)
  • A to G, chromosome 8 at 23,086,977 bp (GRCm38)
  • A to C, chromosome 8 at 41,304,098 bp (GRCm38)
  • A to G, chromosome 8 at 46,508,360 bp (GRCm38)
  • A to G, chromosome 8 at 85,055,359 bp (GRCm38)
  • T to A, chromosome 9 at 5,299,322 bp (GRCm38)
  • T to C, chromosome 9 at 6,337,310 bp (GRCm38)
  • A to G, chromosome 9 at 50,898,758 bp (GRCm38)
  • T to A, chromosome 9 at 89,994,994 bp (GRCm38)
  • T to C, chromosome 9 at 105,784,040 bp (GRCm38)
  • A to G, chromosome 10 at 20,332,146 bp (GRCm38)
  • A to C, chromosome 10 at 30,833,828 bp (GRCm38)
  • G to A, chromosome 10 at 115,408,916 bp (GRCm38)
  • A to G, chromosome 10 at 121,792,674 bp (GRCm38)
  • T to A, chromosome 10 at 121,925,866 bp (GRCm38)
  • T to C, chromosome 10 at 127,238,753 bp (GRCm38)
  • T to A, chromosome 10 at 128,401,741 bp (GRCm38)
  • T to C, chromosome 11 at 3,213,267 bp (GRCm38)
  • T to A, chromosome 11 at 31,058,946 bp (GRCm38)
  • C to T, chromosome 11 at 43,197,512 bp (GRCm38)
  • T to C, chromosome 11 at 49,096,540 bp (GRCm38)
  • C to T, chromosome 11 at 78,931,646 bp (GRCm38)
  • A to G, chromosome 11 at 102,772,669 bp (GRCm38)
  • A to G, chromosome 12 at 36,214,372 bp (GRCm38)
  • T to A, chromosome 12 at 44,598,409 bp (GRCm38)
  • A to T, chromosome 12 at 76,564,593 bp (GRCm38)
  • A to T, chromosome 12 at 84,868,741 bp (GRCm38)
  • A to G, chromosome 12 at 115,648,020 bp (GRCm38)
  • T to A, chromosome 13 at 9,689,208 bp (GRCm38)
  • C to T, chromosome 13 at 93,688,646 bp (GRCm38)
  • T to A, chromosome 13 at 95,684,997 bp (GRCm38)
  • G to T, chromosome 14 at 4,110,527 bp (GRCm38)
  • A to G, chromosome 14 at 55,606,588 bp (GRCm38)
  • C to T, chromosome 14 at 60,691,791 bp (GRCm38)
  • G to A, chromosome 15 at 80,362,117 bp (GRCm38)
  • A to G, chromosome 15 at 83,800,104 bp (GRCm38)
  • C to T, chromosome 15 at 94,283,062 bp (GRCm38)
  • A to G, chromosome 15 at 99,720,123 bp (GRCm38)
  • T to C, chromosome 16 at 4,850,847 bp (GRCm38)
  • T to C, chromosome 16 at 21,766,388 bp (GRCm38)
  • T to A, chromosome 16 at 32,754,698 bp (GRCm38)
  • C to T, chromosome 16 at 36,893,407 bp (GRCm38)
  • T to C, chromosome 16 at 79,087,512 bp (GRCm38)
  • A to G, chromosome 16 at 89,419,888 bp (GRCm38)
  • T to C, chromosome 17 at 18,184,939 bp (GRCm38)
  • T to A, chromosome 17 at 19,677,867 bp (GRCm38)
  • T to A, chromosome 17 at 32,977,297 bp (GRCm38)
  • T to C, chromosome 17 at 63,907,381 bp (GRCm38)
  • A to G, chromosome 18 at 37,817,973 bp (GRCm38)
  • G to A, chromosome 18 at 58,890,415 bp (GRCm38)
  • A to G, chromosome 18 at 65,747,891 bp (GRCm38)
  • A to T, chromosome 19 at 9,010,177 bp (GRCm38)
  • G to A, chromosome 19 at 25,622,639 bp (GRCm38)
  • T to A, chromosome 19 at 29,128,082 bp (GRCm38)
  • C to A, chromosome 19 at 29,754,511 bp (GRCm38)
  • T to G, chromosome 19 at 40,611,299 bp (GRCm38)
  • A to G, chromosome 19 at 47,619,907 bp (GRCm38)
  • T to A, chromosome 19 at 56,858,669 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9747 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069993-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.