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Strain Name:
C57BL/6J-MtgxR9748Btlr/Mmmh
Stock Number:
069994-MU
Citation ID:
RRID:MMRRC_069994-MU
Other Names:
R9748 (G1)
Major Collection:

Strain Information

Tyr
Name: tyrosinase
Synonyms: skc35, Oca1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22173
Homologene: 30969
Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Pak1
Name: p21 (RAC1) activated kinase 1
Synonyms: PAK-1, Paka
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18479
HGNC: HGNC:8590
Homologene: 1936
Camkk2
Name: calcium/calmodulin-dependent protein kinase kinase 2, beta
Synonyms: 6330570N16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 207565
HGNC: HGNC:1470
Homologene: 32756
Prkcd
Name: protein kinase C, delta
Synonyms: PKCdelta, PKC[d], Pkcd, D14Ertd420e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18753
HGNC: HGNC:9399
Homologene: 55963
Wee1
Name: WEE 1 homolog 1 (S. pombe)
Synonyms: Wee1A
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22390
Homologene: 31152
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to G, chromosome 1 at 38,051,160 bp (GRCm38)
  • A to G, chromosome 1 at 40,310,336 bp (GRCm38)
  • G to T, chromosome 1 at 44,056,664 bp (GRCm38)
  • G to A, chromosome 1 at 126,026,202 bp (GRCm38)
  • A to G, chromosome 1 at 135,998,598 bp (GRCm38)
  • A to G, chromosome 1 at 140,162,949 bp (GRCm38)
  • A to G, chromosome 1 at 162,707,866 bp (GRCm38)
  • T to C, chromosome 1 at 166,269,320 bp (GRCm38)
  • A to T, chromosome 1 at 171,601,154 bp (GRCm38)
  • C to T, chromosome 1 at 172,584,233 bp (GRCm38)
  • C to A, chromosome 2 at 28,469,582 bp (GRCm38)
  • C to T, chromosome 2 at 49,694,847 bp (GRCm38)
  • A to G, chromosome 2 at 75,676,323 bp (GRCm38)
  • C to T, chromosome 2 at 91,045,478 bp (GRCm38)
  • C to T, chromosome 2 at 91,485,771 bp (GRCm38)
  • T to A, chromosome 2 at 110,658,295 bp (GRCm38)
  • T to A, chromosome 2 at 118,417,249 bp (GRCm38)
  • T to G, chromosome 2 at 178,383,511 bp (GRCm38)
  • T to G, chromosome 3 at 19,989,171 bp (GRCm38)
  • C to T, chromosome 3 at 67,592,577 bp (GRCm38)
  • T to A, chromosome 3 at 84,666,386 bp (GRCm38)
  • T to G, chromosome 3 at 87,457,162 bp (GRCm38)
  • A to G, chromosome 3 at 93,397,454 bp (GRCm38)
  • G to A, chromosome 3 at 94,175,848 bp (GRCm38)
  • T to A, chromosome 3 at 123,627,982 bp (GRCm38)
  • A to T, chromosome 3 at 130,620,353 bp (GRCm38)
  • T to A, chromosome 4 at 6,269,216 bp (GRCm38)
  • T to C, chromosome 4 at 41,123,538 bp (GRCm38)
  • T to C, chromosome 4 at 123,924,865 bp (GRCm38)
  • C to T, chromosome 4 at 126,323,651 bp (GRCm38)
  • A to G, chromosome 4 at 143,853,322 bp (GRCm38)
  • T to C, chromosome 5 at 30,383,654 bp (GRCm38)
  • A to G, chromosome 5 at 106,975,539 bp (GRCm38)
  • A to T, chromosome 5 at 120,867,025 bp (GRCm38)
  • T to C, chromosome 5 at 121,817,811 bp (GRCm38)
  • G to C, chromosome 5 at 122,734,119 bp (GRCm38)
  • A to T, chromosome 5 at 143,727,778 bp (GRCm38)
  • A to G, chromosome 5 at 143,882,213 bp (GRCm38)
  • A to G, chromosome 6 at 24,619,534 bp (GRCm38)
  • A to T, chromosome 6 at 54,689,004 bp (GRCm38)
  • T to C, chromosome 7 at 29,935,326 bp (GRCm38)
  • T to A, chromosome 7 at 30,254,041 bp (GRCm38)
  • T to A, chromosome 7 at 45,280,517 bp (GRCm38)
  • A to G, chromosome 7 at 87,492,864 bp (GRCm38)
  • GGCG to GGCGACGGCCGCG, chromosome 7 at 97,579,906 bp (GRCm38)
  • C to A, chromosome 7 at 97,898,635 bp (GRCm38)
  • G to T, chromosome 7 at 110,122,515 bp (GRCm38)
  • A to G, chromosome 7 at 114,117,394 bp (GRCm38)
  • A to G, chromosome 8 at 12,649,758 bp (GRCm38)
  • T to C, chromosome 8 at 24,811,052 bp (GRCm38)
  • G to A, chromosome 8 at 105,331,691 bp (GRCm38)
  • T to C, chromosome 8 at 109,646,923 bp (GRCm38)
  • T to A, chromosome 9 at 38,085,057 bp (GRCm38)
  • C to T, chromosome 9 at 52,143,931 bp (GRCm38)
  • C to T, chromosome 9 at 58,823,713 bp (GRCm38)
  • T to G, chromosome 9 at 59,393,298 bp (GRCm38)
  • T to C, chromosome 9 at 75,184,683 bp (GRCm38)
  • A to T, chromosome 10 at 29,148,402 bp (GRCm38)
  • G to A, chromosome 10 at 80,528,573 bp (GRCm38)
  • A to C, chromosome 10 at 89,640,932 bp (GRCm38)
  • T to C, chromosome 11 at 48,945,952 bp (GRCm38)
  • A to G, chromosome 11 at 59,765,522 bp (GRCm38)
  • C to T, chromosome 11 at 59,802,154 bp (GRCm38)
  • T to A, chromosome 11 at 66,085,464 bp (GRCm38)
  • T to C, chromosome 11 at 70,969,671 bp (GRCm38)
  • A to T, chromosome 11 at 73,283,673 bp (GRCm38)
  • A to T, chromosome 11 at 77,116,196 bp (GRCm38)
  • C to A, chromosome 11 at 78,217,941 bp (GRCm38)
  • A to G, chromosome 11 at 109,490,626 bp (GRCm38)
  • C to T, chromosome 12 at 21,274,875 bp (GRCm38)
  • G to T, chromosome 13 at 22,008,334 bp (GRCm38)
  • T to A, chromosome 13 at 120,026,664 bp (GRCm38)
  • G to T, chromosome 14 at 4,110,527 bp (GRCm38)
  • A to T, chromosome 14 at 30,598,843 bp (GRCm38)
  • G to A, chromosome 14 at 30,987,157 bp (GRCm38)
  • G to A, chromosome 15 at 11,310,542 bp (GRCm38)
  • A to G, chromosome 15 at 66,847,159 bp (GRCm38)
  • A to G, chromosome 15 at 74,707,376 bp (GRCm38)
  • G to T, chromosome 15 at 83,114,396 bp (GRCm38)
  • G to T, chromosome 15 at 85,820,670 bp (GRCm38)
  • C to T, chromosome 17 at 3,511,165 bp (GRCm38)
  • T to A, chromosome 17 at 5,899,395 bp (GRCm38)
  • T to C, chromosome 17 at 6,241,205 bp (GRCm38)
  • T to A, chromosome 17 at 7,773,097 bp (GRCm38)
  • A to G, chromosome 17 at 18,243,727 bp (GRCm38)
  • T to G, chromosome 17 at 37,445,704 bp (GRCm38)
  • T to C, chromosome 18 at 20,539,704 bp (GRCm38)
  • T to A, chromosome 18 at 82,618,883 bp (GRCm38)
  • G to T, chromosome 19 at 6,854,093 bp (GRCm38)
  • A to G, chromosome 19 at 37,174,526 bp (GRCm38)
  • T to C, chromosome 19 at 47,268,654 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9748 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069994-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.