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Strain Name:
C57BL/6J-MtgxR9795Btlr/Mmmh
Stock Number:
070040-MU
Citation ID:
RRID:MMRRC_070040-MU
Other Names:
R9795 (G1)
Major Collection:

Strain Information

Mitf
Name: melanogenesis associated transcription factor
Synonyms: mi, wh, BCC2, bHLHe32, Gsfbcc2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17342
HGNC: HGNC:7105
Homologene: 4892
Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Ano1
Name: anoctamin 1, calcium activated chloride channel
Synonyms: Tmem16a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101772
Homologene: 75079
Adgrl3
Name: adhesion G protein-coupled receptor L3
Synonyms: lectomedin 3, LEC3, D130075K09Rik, 5430402I23Rik, Lphn3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319387
Homologene: 22878
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, XAP8, Hbxap, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233532
Homologene: 41142
Qrich1
Name: glutamine-rich 1
Synonyms: 2610028H07Rik, b2b2404Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69232
Homologene: 9803
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 97,744,097 bp (GRCm38)
  • G to A, chromosome 1 at 150,732,938 bp (GRCm38)
  • A to G, chromosome 1 at 181,209,247 bp (GRCm38)
  • T to A, chromosome 2 at 86,958,775 bp (GRCm38)
  • T to C, chromosome 2 at 89,667,467 bp (GRCm38)
  • C to A, chromosome 2 at 91,263,815 bp (GRCm38)
  • T to C, chromosome 2 at 112,110,985 bp (GRCm38)
  • T to C, chromosome 2 at 117,287,948 bp (GRCm38)
  • T to C, chromosome 2 at 118,757,303 bp (GRCm38)
  • T to C, chromosome 2 at 121,290,823 bp (GRCm38)
  • T to A, chromosome 2 at 134,530,632 bp (GRCm38)
  • C to T, chromosome 3 at 37,320,075 bp (GRCm38)
  • C to T, chromosome 3 at 90,389,568 bp (GRCm38)
  • T to C, chromosome 3 at 155,187,676 bp (GRCm38)
  • A to G, chromosome 5 at 33,846,145 bp (GRCm38)
  • C to A, chromosome 5 at 53,172,582 bp (GRCm38)
  • A to G, chromosome 5 at 63,806,889 bp (GRCm38)
  • T to C, chromosome 5 at 81,689,574 bp (GRCm38)
  • T to A, chromosome 5 at 87,564,796 bp (GRCm38)
  • A to T, chromosome 6 at 34,746,136 bp (GRCm38)
  • A to G, chromosome 6 at 69,043,185 bp (GRCm38)
  • A to G, chromosome 6 at 97,993,182 bp (GRCm38)
  • ATGGCG to ATGGCGACGGTGGCG, chromosome 7 at 97,579,904 bp (GRCm38)
  • A to G, chromosome 7 at 108,622,662 bp (GRCm38)
  • A to G, chromosome 7 at 121,648,276 bp (GRCm38)
  • A to T, chromosome 7 at 144,621,697 bp (GRCm38)
  • T to C, chromosome 8 at 112,881,725 bp (GRCm38)
  • T to C, chromosome 9 at 19,497,116 bp (GRCm38)
  • T to A, chromosome 9 at 19,884,051 bp (GRCm38)
  • CGGG to CGG, chromosome 9 at 35,210,856 bp (GRCm38)
  • C to T, chromosome 9 at 58,853,479 bp (GRCm38)
  • T to A, chromosome 9 at 108,533,890 bp (GRCm38)
  • T to C, chromosome 10 at 24,181,577 bp (GRCm38)
  • T to C, chromosome 10 at 34,126,548 bp (GRCm38)
  • T to C, chromosome 10 at 75,798,557 bp (GRCm38)
  • T to C, chromosome 10 at 88,921,729 bp (GRCm38)
  • T to G, chromosome 10 at 89,478,789 bp (GRCm38)
  • A to T, chromosome 11 at 49,495,055 bp (GRCm38)
  • T to A, chromosome 11 at 98,211,225 bp (GRCm38)
  • A to T, chromosome 12 at 84,829,354 bp (GRCm38)
  • T to C, chromosome 14 at 65,609,896 bp (GRCm38)
  • C to T, chromosome 14 at 84,532,910 bp (GRCm38)
  • T to A, chromosome 15 at 10,537,645 bp (GRCm38)
  • T to C, chromosome 15 at 44,543,587 bp (GRCm38)
  • G to T, chromosome 15 at 74,958,541 bp (GRCm38)
  • C to T, chromosome 15 at 94,403,299 bp (GRCm38)
  • C to A, chromosome 15 at 101,802,290 bp (GRCm38)
  • T to A, chromosome 16 at 59,216,575 bp (GRCm38)
  • T to C, chromosome 16 at 63,552,547 bp (GRCm38)
  • T to A, chromosome 17 at 18,947,299 bp (GRCm38)
  • T to G, chromosome 17 at 21,461,789 bp (GRCm38)
  • T to C, chromosome 17 at 55,592,496 bp (GRCm38)
  • A to T, chromosome 17 at 58,102,197 bp (GRCm38)
  • T to A, chromosome 18 at 34,314,575 bp (GRCm38)
  • C to A, chromosome 18 at 61,005,794 bp (GRCm38)
  • T to C, chromosome 18 at 61,741,751 bp (GRCm38)
  • A to G, chromosome 18 at 74,305,670 bp (GRCm38)
  • C to A, chromosome 19 at 53,864,120 bp (GRCm38)
  • T to C, chromosome Y at 1,364,679 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9795 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
070040-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.