Loading Mouse GIF
Loading...

Strain Name:
B6J;129S1-Rorbtm1.1Df/Mmucd
Stock Number:
071442-UCD
Citation ID:
RRID:MMRRC_071442-UCD
Other Names:
RORb1-GFP, Rorb1g, RorbGFP

Strain Information

Rorbtm1.1Df
Name: RAR-related orphan receptor beta; targeted mutation 1.1, Douglas Forrest
Synonyms: RorbGFP, Rorb1g
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knock-In
Rorb
Name: RAR-related orphan receptor beta
Synonyms: RZR-beta, Nr1f2, Rorbeta, hstp
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Knock-In
NCBI: 225998
Homologene: 38250
Genetic Alterations
An enhanced, targeted GFP cDNA replaced the coding sequence in RORβ1-specific exon 1. The final allele lacks the self-excising neomycin resistance cassette inserted downstream of the GFP gene.
ES Cell Line
W9.5/W95
Phenotype
Homozygotes show characteristic gait phenotype referred to as “duck gait”. In the retina, there was a severe loss of horizontal and amacrine cells coupled with increased ganglion cell numbers. This suggests that RORβ1, like Ptf1a, may influence a pathway with alternative differentiation outcomes of ganglion cell versus amacrine and horizontal cells
Mice do not express isoform beta 1, but do express isoform beta 2.

This strain may be useful for studying interneuron differentiation.
MeSH Terms
  • Alleles
  • Amacrine Cells/cytology
  • Amacrine Cells/metabolism
  • Animals
  • Base Sequence
  • Cell Differentiation/genetics
  • Enhancer Elements, Genetic/genetics
  • Gene Deletion
  • Gene Expression Regulation
  • Mice
  • Mice, Inbred C57BL
  • Models, Biological
  • Molecular Sequence Data
  • Nuclear Receptor Subfamily 1, Group F, Member 2/genetics
  • Nuclear Receptor Subfamily 1, Group F, Member 2/metabolism
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Retinal Ganglion Cells/cytology
  • Retinal Ganglion Cells/metabolism
  • Retinal Horizontal Cells/cytology
  • Retinal Horizontal Cells/metabolism
  • Transcription Factors/genetics
Strain Development
Genomic Rorb 5′ and 3′ homology arms isolated from ES cell (derived from 129/Sv-+p+Tyr-cMgfSl-J/+ mice) DNA were inserted into a pACN vector carrying a self-excisable neomycin-resistance gene (tACE-Cre-Neo). An enhanced gfp cDNA replaced the RORβ1-specific exon. Targeted W9.5 ES cell clones were determined by Southern blot and used to generate chimeric mice. Chimeras were crossed with C57BL/6J mice, the ACN cassette self-excised during germline transmission from male chimeras to yield the Rorb1g allele. Tail-DNA PCR to confirm genotype. After backcrossing for 2 generations onto C57BL/6J, the Rorb+/1g mice intercrossed to generate +/+, +/1g and 1g/1g progeny for analysis.
Suggested Control Mice
wild-type animal of C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Neurobiology
  • Sensorineural
Donor
Douglas Forrest, Ph.D., NIDDK, NIH.
Hong Liu, Ph.D., NIDDK, NIH.
Primary Reference

Liu H, Kim SY, Fu Y, Wu X, Ng L, Swaroop A, Forrest D. An isoform of retinoid-related orphan receptor β directs differentiation of retinal amacrine and horizontal interneurons. Nat Commun. 2013;4:1813. doi: 10.1038/ncomms2793. (Medline PMID: 23652001)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Reduced
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 to 12 months Variable
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
071442-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.