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Strain Detail Sheet

Strain Name:
B6J.129(Cg)-Rr27tm1Kpfe/Mmjax
Stock Number:
071834-JAX
Citation ID:
RRID:MMRRC_071834-JAX
Other Names:
H19-ICRFlox

Strain Information

Gene Details

Rr27tm1Kpfe
Name: regulatory region 27; targeted mutation 1, Karl Pfeifer
Synonyms: H19tm1Kpfe, DMRflox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Conditional
Rr27
Name: regulatory region 27
Synonyms: IC1, ICR, DMD
Type: Regulatory Region
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Conditional
Genetic Alterations
The differentially methylated region (DMR), located upstream of the H19 transcription initiation site, was left flanked by single loxP sites at -6.5 to -0.8 kb upstream of H19 major start within the regulatory region 27 (Rr27) and a neomycin-resistance gene flanked by loxP sites inserted at the -7.0kb upstream of H19. The neo-cassette was removed in ES cells by transiently transfecting with a Cre recombinase-expressing plasmid.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+

Strain Description

Phenotype
In this strain, flox sites flank the H19/Igf2 Imprinting Control Region. There is no phenotype associated with these loxP insertions, but upon cre recombination on the maternal chromosome there is a phenotype that mimics Beckwith Wiedemann syndrome. Maternal Igf2 expression is activated so that overall there is 2X expression of Igf2 and maternal H19 expression is reduced by 4-50-fold (tissue dependent manner). Together, these expression changes result in a series of phenotypes including fetal overgrowth, cancer susceptibility, and cardiac insufficiency in aged mice.

Conditional phenotype: No

MeSH Terms
  • Alleles
  • Animals
  • Attachment Sites, Microbiological/genetics
  • Chromosomes/genetics
  • CpG Islands/genetics
  • DNA Methylation
  • Female
  • Gametogenesis/genetics
  • Gene Expression Regulation, Developmental/genetics
  • Gene Silencing
  • Genomic Imprinting/genetics
  • Insulin-Like Growth Factor II/genetics
  • Integrases/genetics
  • Integrases/metabolism
  • Male
  • Mice
  • Models, Genetic
  • Muscle Proteins/genetics
  • Mutagenesis, Insertional/genetics
  • Promoter Regions, Genetic/genetics
  • RNA, Long Noncoding
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • RNA, Untranslated
  • Regulatory Sequences, Nucleic Acid/genetics
  • Sequence Deletion/genetics
  • Time Factors
  • Transcription, Genetic/genetics
  • Viral Proteins
Strain Development
The targeting vector was constructed by use of DNA derived from 129/SvJ mice. The vector contained sequences extending from −11-kb EcoRI to +6.5-kb XbaI relative to the H19 transcriptional start site. Additionally, it carried a neomycin-resistance gene flanked by loxP sites inserted at the −7.0-kb HindIII site, another loxP site inserted at the −0.8-kb XbaI site, and a diptheria toxin gene as a negative marker. The targeting vector was linearized and electroporated into mouse R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl+) embryonic stem cells. Correctly targeted clones were confirmed by Southern hybridization and positive clones were electroporated with plasmid pBS185 directly expressing cre recombinase to excise the Neor gene. Clones carrying the DMRflox allele were introduced into C57BL/6J blastocysts to generate chimeric founder mice. The neo-cassette was removed in ES cells by transiently transfecting with a Cre recombinase-expressing plasmid. This mouse (without NeoR sequences) was then backcrossed 8 times to C57BL/6J.
Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cardiovascular
  • Developmental Biology
  • Models for Human Disease

Strain Origin

Donor
Karl Pfeifer, National Instititutes of Health (NIH), Eunice Kennedy Shriver National Institute of Child Health and.
Primary Reference

Srivastava M, Hsieh S, Grinberg A, Williams-Simons L, Huang SP, Pfeifer K. H19 and Igf2 monoallelic expression is regulated in two distinct ways by a shared cis acting regulatory region upstream of H19. Genes Dev. 2000 May 15;14(10):1186-95. (Medline PMID: 10817754)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
8
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Undetermined
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Nathan Whitman.

Conditions of Distribution

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Nathan Whitman

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
071834-JAX-SPERM Cryo-preserved spermatozoa $459.00 / $459.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
071834-JAX-RESUS Litter recovered from cryo-archive $2,123.00 / $2,123.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.