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Strain Detail Sheet

Strain Name:
hACE2/hTMPRSS2/Slc6a20_5UTR.G>T
Stock Number:
071888-UCD
Citation ID:
RRID:MMRRC_071888-UCD
Other Names:
C57BL/6N-Ace2em1(ACE2)MbpTmprss2em1(TMPRSS2)MbpSlc6a20bem1Mbp/Mmucd
Major Collection:
Precision Genetically-Humanized Models

Strain Information

Gene Details

Ace2em1(ACE2)Mbp
Name: angiotensin converting enzyme 2; endonuclease-mediated mutation 1, Mouse Biology Program, UC Davis
Type: Allele
Species: Multi-species
Chromosome: X
Alteration at locus: CRISPR
Tmprss2em1(TMPRSS2)Mbp
Name: transmembrane protease, serine 2; endonuclease-mediated mutation 1, Mouse Biology Program, UC Davis
Type: Allele
Species: Multi-species
Chromosome: 16
Alteration at locus: CRISPR
Ace2
Name: angiotensin converting enzyme 2
Synonyms: 2010305L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: CRISPR
NCBI: 70008
Homologene: 41448

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tmprss2
Name: transmembrane protease, serine 2
Synonyms: epitheliasin, D16Ertd61e
Type: Gene
Species: Mouse
Chromosome: 16
Alteration at locus: CRISPR
NCBI: 50528
VEGA: 16
Homologene: 4136

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Slc6a20b
Name: solute carrier family 6 (neurotransmitter transporter), member 20B
Synonyms: XT3, Xtrp3, Sit1, Slc6a20
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: CRISPR
NCBI: 22599
Homologene: 130652

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Slc6a20b_5UTRG>T was created in the mouse to model the human 5’UTR variant caused by rs2271616. CRISPR RNP using guide CCTCGCTGCCTCAACCTCAC was used to assist with homology directed repair (HDR) using an ssODN repair template with the following single G>T SNP edit: AACCTCACAG(G/T)TGGCCAGTTT in the mouse 5’UTR and electroporated in mouse C57BL/6N-Ace2em1(ACE2)Mbp Tmprss2em1(TMPRSS2)Mbp zygotes. Founder animals were screened by QPCR MGB probe with positives subsequently PCR’d and bidirectionally sequenced at the loci.
Genotype Determination
Genotyping Protocol
ES Cell Line
Not applicable

Strain Description

Phenotype
Unknown
Strain Development
Slc6a20b_5UTRG>T was created in the mouse to model the human 5’UTR variant caused by rs2271616. CRISPR RNP using guide CCTCGCTGCCTCAACCTCAC was used to assist with homology directed repair (HDR) using an ssODN repair template with the following single G>T SNP edit: AACCTCACAG(G/T)TGGCCAGTTT in the mouse 5’UTR and electroporated in mouse C57BL/6N-Ace2em1(ACE2)Mbp Tmprss2em1(TMPRSS2)Mbp zygotes. Founder animals were screened by QPCR MGB probe with positives subsequently PCR’d and bidirectionally sequenced at the loci. Sequence confirmed founder animals were then backcrossed to C57BL/6N-Ace2em1(ACE2)Mbp Tmprss2em1(TMPRSS2)Mbp and subsequence N1 Slc6a20b_5UTRG>T heterozygous animals were confirmed for copy number as well as sequence analysis. Potential off targets with an MIT score greater than 1.5 were not found.
Suggested Control Mice
C57BL/6N or wild-type siblings

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Immunology and Inflammation
  • Models for Human Disease

Strain Origin

Donor
Kent Lloyd, D.V.M., University of California, Davis.
Primary Reference
Not ready to publish

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Sib-mating
Generation
Unknown
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes X-linked
Homozygotes are fertile: Yes X-linked
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4 to 6
Recommended wean age
4 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.