Strain Detail Sheet

Strain Name    :

B6.129S4-Bcl2l2Gt(ROSA)41Sor/Mmmh

Stock Number :

009983-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Bcl2l2Gt(ROSA)41Sor
Name: gene trap ROSA 41, Philippe Soriano
Alteration at locus: Gene Trap
Gene Symbol: Bcl2l2
Name: BCL2-like 2
Chromosome: 14
Alteration at locus: Gene Trap

Genetic Alterations:
A retroviral gene trap (ROSA b-gal) inserted within intron 2 of the Bcl2l2 (Bclw) locus.

Genotype Determination:

ES Cell Line: AK7 derived from 129S4/SvJae

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

  • On a B6 background, homozygous mutant animals are not recovered with Mendelian ratios, suggesting loss during embryogenesis, early post-natal. Homozygous mutants are often runted, females display loss of oocytes from ovary and preneoplastic transformation of ovarian surface epithelium.  
  • On 129S4 background, MMRRC:009984, males and females have problems with eyes - microphthalmia and lacrimal gland problems. 
  • On B6;129S4 hybrid background, males are sterile due to loss of germ cells and Sertoli cells from testis. 


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Bcl2l2Gt(ROSA)41Sor/Bcl2l2Gt(ROSA)41Sor
        involves: 129S5/SvEvBrd
  • endocrine/exocrine gland phenotype
    • abnormal seminiferous tubule morphology (MGI Ref ID J:46386)
      • tubules are devoid of germ cells by 6 months of age
      • by 8 months of age most sertoli cells had sloughed off and there was reduction in the number of leydig cells
    • decreased seminal vesicle weight (MGI Ref ID J:46386)
      • progressive weight reduction after 5 months of age, resulting in weights 10% those of normal at 14 months of age
    • small testis (MGI Ref ID J:46386)
  • nervous system phenotype
    • abnormal CNS synapse formation (MGI Ref ID J:137190)
      • mismatched connections between pre- and postsynaptic active zones of Purkinje cell/parallel fiber synapses are frequently seen
      • other synaptic defects such as shortened active zones and thickened postsynaptic densities
    • abnormal Purkinje cell dendrite morphology (MGI Ref ID J:137190)
      • large numbers of naked dendritic spines are present
  • behavior/neurological phenotype
    • abnormal motor learning (MGI Ref ID J:137190)
      • mice fail to improve performance in rotating rod trials in contrast to wild-type animals
  • reproductive system phenotype
    • abnormal seminiferous tubule morphology (MGI Ref ID J:46386)
      • tubules are devoid of germ cells by 6 months of age
      • by 8 months of age most sertoli cells had sloughed off and there was reduction in the number of leydig cells
    • decreased male germ cell number (MGI Ref ID J:46386)
      • degenerating spermatocytes are first observed at 19 days of age
      • 10-fold decrease in the number of spermatids by 24 days of age
      • all stages of developing germ cells were observed at 2.5 months of age, though most were lost by 5 months of age
    • decreased seminal vesicle weight (MGI Ref ID J:46386)
      • progressive weight reduction after 5 months of age, resulting in weights 10% those of normal at 14 months of age
    • male infertility (MGI Ref ID J:46386)
    • small testis (MGI Ref ID J:46386)
  • cellular phenotype
    • abnormal mitochondrial morphology (MGI Ref ID J:137190)
      • mitochondria lengths in Purkinje cells are 1.4-1.5 um, compared to lengths of 0.8-1.0 um in wild-type animals
      • often, points where mitochondria become constricted are observed in mutants but not in wild-type mitochondria, suggestive of impaired fission
  • homeostasis/metabolism phenotype
    • increased circulating follicle stimulating hormone level (MGI Ref ID J:46386)
      • increased levels observed between 2.5 and 15 months of age
Bcl2l2Gt(ROSA)41Sor/Bcl2l2Gt(ROSA)41Sor
        involves: 129S5/SvEvBrd * C57BL/6J
  • endocrine/exocrine gland phenotype
    • Leydig cell hyperplasia (MGI Ref ID J:70212)
      • increased number of leydig cells relative to wild-type between 3 and 8 months of age
    • Sertoli cell hypoplasia (MGI Ref ID J:70212)
      • progressive reduction in the number of cells beginning at 23 days of age
      • 60% reduction relative to wild-type at 90 days of age and reaching 0% in some cases by 12 months of age
      • an intact blood-testes barrier was observed at 90 days of age, but not at 5.5 months of age
    • absent Leydig cells (MGI Ref ID J:70212)
      • following hyperplasia, a gradual loss of cells was observed from 8 to 12 months, at which point few to no Leydig cells were observed
    • decreased seminal vesicle weight (MGI Ref ID J:70212)
      • progressive weight reduction after 7.5 months of age, resulting in weights 10% those of normal at 12 months of age
    • decreased testis weight (MGI Ref ID J:70212)
      • progressive weight reduction first evident at 34 days of age
      • testicular weight was 12 to 20% that of wild-type at 9 months of age
    • small seminiferous tubules (MGI Ref ID J:70212)
      • reduction of luminal volume
  • reproductive system phenotype
    • Leydig cell hyperplasia (MGI Ref ID J:70212)
      • increased number of leydig cells relative to wild-type between 3 and 8 months of age
    • Sertoli cell hypoplasia (MGI Ref ID J:70212)
      • progressive reduction in the number of cells beginning at 23 days of age
      • 60% reduction relative to wild-type at 90 days of age and reaching 0% in some cases by 12 months of age
      • an intact blood-testes barrier was observed at 90 days of age, but not at 5.5 months of age
    • absent Leydig cells (MGI Ref ID J:70212)
      • following hyperplasia, a gradual loss of cells was observed from 8 to 12 months, at which point few to no Leydig cells were observed
    • decreased germ cell number (MGI Ref ID J:70212)
    • decreased seminal vesicle weight (MGI Ref ID J:70212)
      • progressive weight reduction after 7.5 months of age, resulting in weights 10% those of normal at 12 months of age
    • decreased testis weight (MGI Ref ID J:70212)
      • progressive weight reduction first evident at 34 days of age
      • testicular weight was 12 to 20% that of wild-type at 9 months of age
    • small seminiferous tubules (MGI Ref ID J:70212)
      • reduction of luminal volume

Strain of Origin: 129S4 ES cells

Strain genetic background: C57BL/6JSorMgr

Suggested Control Mice:

  • Wild-type littermates or C57BL/6

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Models for Human Disease
  • Neurobiology
  • Reproduction
  • Research Tools

Strain Origin

Donor: Grant MacGregor, Ph.D., University of California, Irvine.

Primary Reference:

Ross AJ, Waymire KG, Moss JE, Parlow AF, Skinner MK, Russell LD, MacGregor GR. Testicular degeneration in Bclw-deficient mice. Nat Genet. 1998 Mar;18(3):251-6. (Medline PMID: 9500547).

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • C57BL/6 female x Heterozygous male or reciprocal mating

Generation: N12 (when donated)

Overall Breeding Performance: Excellent

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: 8 months 10 months

Average litter size: 8-10

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
009983-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
009983-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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