Strain Name    : 129S4-Bcl2l2Gt(ROSA)41Sor/Mmmh

Stock Number : 009984-MU

Gene Information

(provided by MGI)
Allele Symbol: Bcl2l2Gt(ROSA)41Sor
Name: gene trap ROSA 41, Philippe Soriano
Chromosome: 14
Alteration at locus: Gene Trap
Gene Symbol: Bcl2l2
Name: BCL2-like 2
Chromosome: 14
Alteration at locus: Gene Trap
Genetic Alterations:
A retroviral gene trap (ROSA b-gal) inserted within intron 2 of the Bcl2l2 (Bclw) locus.

Genotype Determination:

ES Cell Line: AK7

Phenotype

  • On 129S4 background, males and females have problems with eyes - microphthalmia and lacrimal gland problems. 
  • On a B6 (cogenic) background, MMRRC:009983, homozygous mutant animals are not recovered with Mendelian ratios, suggesting loss during embryogenesis, early post-natal. Homozygous mutants are often runted, females display loss of oocytes from ovary and preneoplastic transformation of ovarian surface epithelium.
  • On B6;129S4 hybrid background, males are sterile due to loss of germ cells and Sertoli cells from testis.   

Mammalian Phenotype Terms:
Allelic Composition: Bcl2l2Gt(ROSA)41Sor/Bcl2l2Gt(ROSA)41Sor (Genetic Background: involves: 129S5/SvEvBrd )

Allelic Composition: Bcl2l2Gt(ROSA)41Sor/Bcl2l2Gt(ROSA)41Sor (Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J )

Strain of Origin: 129S4 ES cells

Strain genetic background: 129S4JaeSorMgr

Suggested Control Mice: Wild-type littermates or 129S4

  • Apoptosis
  • Cancer
  • Cell Biology
  • Models for Human Disease
  • Neurobiology
  • Reproduction
  • Research Tools

Donor: Grant MacGregor, Ph.D., University of California, Irvine.

Primary Reference:

Ross AJ, Waymire KG, Moss JE, Parlow AF, Skinner MK, Russell LD, MacGregor GR. Testicular degeneration in Bclw-deficient mice. Nat Genet. 1998 Mar;18(3):251-6. (Medline PMID: 9500547).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Coat color: Black or white bellied agouti

Other:

MMRRC Breeding System: Backcross or Sib-mating

Breeding Scheme(s):

  • 129S4 female x Heterozygous male or reciprocal mating
  • Heterozygous female x Heterozygous sibling

Generation: N12F20 (when donated)

Overall Breeding Performance: Excellent

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 months 12 months

Average litter size: 6

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
009984-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.