Most MMRRC strains are maintained as cryo-archives, in one or more formats (embryos, spermatazoa, ovaries or ES cell line); the MMRRC can distribute cryopreserved materials for reanimation at your facility (or by a FIMRe member for shipment to your facility). Researchers requesting materials for reanimation at their facility should confirm that they have sufficient expertise in the recovery technologies.
The standard quantities of cryopreserved materials shipped are sufficient for at least two embryo transfer recipients when performed following the MMRRC's recovery protocols. If you are not sufficiently experienced in recovering mice from cryopreserved materials, we recommend that you either:
While the MMRRC facilities can supply technical support, we cannot guarantee successful reanimation when recovery is performed outside our own facilities. (If there is a failure, the MMRRC will only refund the distribution fee if we verify that the cryopreservation of the materials was faulty.)