Register New MMRRC Account
Reset Password
Register for New Mouse Models
Click Here for Additional Contact Information
Availability & Fees Order this Strain
Genotyping Protocol
Homozygous: Newborns present one or more gross defects with incomplete penetrance. About 30% of the litter are smaller in stature (runt) following a cross between mice that is homozygous and heterozygous (tuft/tuft x tuft/+) for the mutation. But some grow to normal weight following one month in age. They are otherwise viable. Mice can also present one or more gross craniofacial malformations such as midface cleft (nasal), anterior cranial cephalocele, ocular hypertelorism, sloping nasal ridge. The occurrence rate is about 20%. About a third die perinatally of unknown cause or from earlier defects to neural tube closure resulting in the occurrence of exencephaly/anencephaly and possibly during gastrulation/implantation. Milder forms present incomplete closure of the anterior neuropore, but their fate is unclear (PMID:24931720 and 26989192).
Hetero/Hemizygous: Mice heterozygous for the mutation appear normal. However, we have found a small percentage (about 5%) of mice with one of the typical traits for mice homozygous for the mutation to be heterozygous. Therefore, we believe the mutation can have a dominant negative effect. Neomorphic effects have not yet been ruled out. This is described in PMID:26989192.
To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.
The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.
Fong KS, Cooper TB, Drumhiller WC, Somponpun SJ, Yang S, Ernst T, Chang L,Lozanoff S. Craniofacial features resembling frontonasal dysplasia with atubulonodular interhemispheric lipoma in the adult 3H1 tuft mouse. Birth Defects Res A Clin Mol Teratol. 2012 Feb;94(2):102-13. doi: 10.1002/bdra.22878. Epub 2012Jan 13. (Medline PMID: 22246904)
Fong KS, Adachi DA, Chang SB, Lozanoff S. Midline craniofacial malformations with a lipomatous cephalocele are associated with insufficient closure of the neural tube in the tuft mouse. Birth Defects Res A Clin Mol Teratol. 2014 Aug;100(8):598-607. doi: 10.1002/bdra.23264. Epub 2014 Jun 13. (Medline PMID: 24931720)
Fong KS, Hufnagel RB, Khadka VS, Corley MJ, Maunakea AK, Fogelgren B, AhmedZM, Lozanoff S. A mutation in the tuft mouse disrupts TET1 activity and altersthe expression of genes that are crucial for neural tube closure. Dis Model Mech.2016 May 1;9(5):585-96. doi: 10.1242/dmm.024109. Epub 2016 Mar 17. (Medline PMID: 26989192)
Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The submitter or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
We use cookies to improve your experience, personalize content, and analyze traffic. By using this site, you agree to our use of cookies. For more details, please visit our Privacy Policy.