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Strain Name:
C57BL/6J-MtgxR5979Btlr/Mmmh
Stock Number:
044161-MU
Citation ID:
RRID:MMRRC_044161-MU
Other Names:
R5979 (G1)
Major Collection:

Strain Information

Sall4
Name: spalt like transcription factor 4
Synonyms: 5730441M18Rik, Tex20, C330011P20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99377
Homologene: 10716
Ttr
Name: transthyretin
Synonyms: prealbumin
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 22139
VEGA: 18
Homologene: 317
Casp8
Name: caspase 8
Synonyms: Mch5, MACH, FLICE, Caspase-8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12370
HGNC: HGNC:1509
Homologene: 7657
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Lgi3
Name: leucine-rich repeat LGI family, member 3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 213469
VEGA: 14
Homologene: 16406
Dhx35
Name: DEAH-box helicase 35
Synonyms: Ddx35, 1200009D07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71715
Homologene: 6406
Swt1
Name: SWT1 RNA endoribonuclease homolog (S. cerevisiae)
Synonyms: 1200016B10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66875
Homologene: 32355
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 11,132,372 bp
  • G to T, chromosome 1 at 30,805,746 bp
  • T to A, chromosome 1 at 34,160,372 bp
  • T to C, chromosome 1 at 55,052,918 bp
  • A to T, chromosome 1 at 58,828,912 bp
  • A to T, chromosome 1 at 59,702,831 bp
  • A to T, chromosome 1 at 86,090,053 bp
  • G to T, chromosome 1 at 105,589,274 bp
  • A to G, chromosome 1 at 123,384,283 bp
  • A to G, chromosome 1 at 128,211,223 bp
  • C to A, chromosome 1 at 140,118,671 bp
  • A to T, chromosome 1 at 151,407,588 bp
  • A to G, chromosome 1 at 152,557,493 bp
  • A to G, chromosome 1 at 190,800,435 bp
  • T to C, chromosome 2 at 119,069,360 bp
  • C to T, chromosome 2 at 120,946,382 bp
  • G to A, chromosome 2 at 131,516,414 bp
  • A to G, chromosome 2 at 155,522,109 bp
  • A to C, chromosome 2 at 156,097,759 bp
  • G to T, chromosome 2 at 158,842,869 bp
  • A to G, chromosome 2 at 165,397,547 bp
  • A to T, chromosome 2 at 168,750,343 bp
  • C to T, chromosome 3 at 59,336,473 bp
  • A to G, chromosome 3 at 123,117,411 bp
  • T to C, chromosome 4 at 41,715,393 bp
  • C to T, chromosome 4 at 46,507,239 bp
  • T to C, chromosome 4 at 106,720,926 bp
  • A to G, chromosome 4 at 107,105,281 bp
  • A to G, chromosome 4 at 132,579,305 bp
  • T to A, chromosome 5 at 21,582,091 bp
  • A to G, chromosome 5 at 89,861,669 bp
  • A to T, chromosome 6 at 3,476,149 bp
  • T to C, chromosome 6 at 42,321,971 bp
  • C to T, chromosome 6 at 48,463,693 bp
  • T to A, chromosome 6 at 113,293,749 bp
  • A to C, chromosome 7 at 7,312,335 bp
  • T to A, chromosome 7 at 20,102,065 bp
  • G to A, chromosome 7 at 107,036,336 bp
  • G to A, chromosome 7 at 107,794,388 bp
  • A to G, chromosome 7 at 131,554,550 bp
  • G to A, chromosome 7 at 139,939,827 bp
  • A to G, chromosome 7 at 141,697,250 bp
  • G to A, chromosome 7 at 141,751,406 bp
  • T to C, chromosome 8 at 24,677,367 bp
  • C to T, chromosome 8 at 27,197,923 bp
  • T to C, chromosome 8 at 70,302,543 bp
  • T to G, chromosome 8 at 122,892,400 bp
  • ATTGCTGTTT to ATTGCTGTTTGCTGTTT, chromosome 9 at 38,026,540 bp
  • A to G, chromosome 9 at 48,396,562 bp
  • G to T, chromosome 9 at 123,479,414 bp
  • T to C, chromosome 10 at 27,235,732 bp
  • T to C, chromosome 10 at 103,022,259 bp
  • C to A, chromosome 11 at 22,151,887 bp
  • A to G, chromosome 11 at 58,646,083 bp
  • A to G, chromosome 11 at 58,841,840 bp
  • T to C, chromosome 11 at 59,548,971 bp
  • T to A, chromosome 11 at 60,538,691 bp
  • A to G, chromosome 11 at 65,834,481 bp
  • T to C, chromosome 13 at 33,255,053 bp
  • A to T, chromosome 13 at 48,491,040 bp
  • G to A, chromosome 13 at 54,739,978 bp
  • A to T, chromosome 13 at 59,534,046 bp
  • G to A, chromosome 13 at 81,665,274 bp
  • T to C, chromosome 13 at 92,661,136 bp
  • G to T, chromosome 13 at 119,776,446 bp
  • G to A, chromosome 14 at 70,536,460 bp
  • A to G, chromosome 15 at 91,772,945 bp
  • G to A, chromosome 16 at 8,636,160 bp
  • A to C, chromosome 16 at 13,606,171 bp
  • A to T, chromosome 16 at 38,575,744 bp
  • G to A, chromosome 16 at 44,832,932 bp
  • T to G, chromosome 16 at 59,434,568 bp
  • G to A, chromosome 16 at 97,875,218 bp
  • C to A, chromosome 17 at 23,557,911 bp
  • G to T, chromosome 17 at 30,815,664 bp
  • T to C, chromosome 17 at 32,198,726 bp
  • A to T, chromosome 17 at 34,841,463 bp
  • T to C, chromosome 17 at 38,305,192 bp
  • T to A, chromosome 17 at 49,459,204 bp
  • C to T, chromosome 17 at 91,088,203 bp
  • T to C, chromosome 18 at 20,670,002 bp
  • T to A, chromosome 18 at 37,437,991 bp
  • G to T, chromosome 18 at 67,421,259 bp
  • T to G, chromosome 19 at 3,884,513 bp
  • T to A, chromosome 19 at 5,381,223 bp
  • G to T, chromosome 19 at 10,443,479 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5979 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044161-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.