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Strain Name:
C57BL/6J-MtgxR5571Btlr/Mmmh
Stock Number:
044395-MU
Citation ID:
RRID:MMRRC_044395-MU
Other Names:
R5571 (G1)
Major Collection:

Strain Information

Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Dhrs3
Name: dehydrogenase/reductase 3
Synonyms: retSDR1, Rsdr1, dehydrogenase/reductase (SDR family) member 3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20148
Homologene: 20994
Hmgcr
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Synonyms: HMG-CoAR, 3-hydroxy-3-methylglutaryl-CoA reductase, Red
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15357
HGNC: HGNC:5006
Homologene: 30994
Ppp4r1
Name: protein phosphatase 4, regulatory subunit 1
Synonyms: Pp4r1, 3110001J10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 70351
HGNC: HGNC:9320
Homologene: 81737
Wdr59
Name: WD repeat domain 59
Synonyms: 5430401O09Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 319481
Homologene: 38685
Atad5
Name: ATPase family, AAA domain containing 5
Synonyms: LOC237877, C130052G03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237877
Homologene: 32611
Zfhx3
Name: zinc finger homeobox 3
Synonyms: WBP9, A230102L03Rik, Atbf1, Sci
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 90,788,216 bp
  • T to C, chromosome 1 at 106,023,672 bp
  • T to C, chromosome 1 at 175,647,194 bp
  • A to G, chromosome 2 at 34,715,253 bp
  • A to G, chromosome 2 at 36,377,117 bp
  • T to C, chromosome 2 at 41,408,342 bp
  • C to G, chromosome 2 at 85,044,325 bp
  • A to T, chromosome 3 at 39,010,274 bp
  • A to T, chromosome 3 at 59,877,219 bp
  • C to T, chromosome 3 at 92,777,681 bp
  • T to C, chromosome 3 at 108,856,408 bp
  • T to C, chromosome 4 at 131,937,406 bp
  • T to G, chromosome 4 at 144,893,564 bp
  • A to G, chromosome 5 at 5,675,912 bp
  • T to C, chromosome 5 at 108,802,240 bp
  • G to T, chromosome 7 at 4,368,900 bp
  • T to C, chromosome 7 at 6,570,825 bp
  • T to G, chromosome 7 at 13,450,915 bp
  • A to G, chromosome 7 at 28,156,569 bp
  • A to G, chromosome 7 at 45,461,891 bp
  • A to G, chromosome 7 at 65,699,124 bp
  • T to C, chromosome 7 at 86,734,120 bp
  • C to T, chromosome 7 at 118,489,869 bp
  • T to A, chromosome 7 at 140,249,123 bp
  • C to T, chromosome 8 at 85,640,544 bp
  • A to T, chromosome 8 at 108,955,991 bp
  • A to T, chromosome 8 at 111,465,831 bp
  • G to A, chromosome 9 at 37,616,923 bp
  • C to T, chromosome 9 at 67,334,320 bp
  • T to G, chromosome 9 at 109,403,088 bp
  • T to C, chromosome 9 at 109,606,998 bp
  • T to C, chromosome 10 at 118,159,683 bp
  • T to A, chromosome 11 at 23,457,975 bp
  • T to A, chromosome 11 at 58,702,051 bp
  • T to C, chromosome 11 at 80,111,556 bp
  • T to C, chromosome 11 at 97,148,729 bp
  • A to G, chromosome 11 at 116,063,654 bp
  • T to A, chromosome 13 at 11,555,448 bp
  • A to G, chromosome 13 at 22,354,998 bp
  • A to T, chromosome 13 at 31,917,721 bp
  • G to A, chromosome 13 at 64,903,758 bp
  • A to T, chromosome 13 at 96,666,663 bp
  • T to C, chromosome 15 at 79,271,583 bp
  • T to C, chromosome 15 at 81,643,217 bp
  • A to T, chromosome 16 at 59,000,206 bp
  • A to T, chromosome 16 at 97,590,871 bp
  • C to T, chromosome 17 at 65,803,861 bp
  • A to G, chromosome 18 at 35,770,478 bp
  • T to C, chromosome 18 at 78,209,067 bp
  • A to G, chromosome 18 at 86,713,148 bp
  • G to A, chromosome 19 at 8,783,930 bp
  • T to A, chromosome 19 at 16,872,767 bp
  • T to C, chromosome 19 at 45,777,006 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5571 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044395-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.