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Strain Name:
C57BL/6J-MtgxR5766Btlr/Mmmh
Stock Number:
044424-MU
Citation ID:
RRID:MMRRC_044424-MU
Other Names:
R5766 (G1)
Major Collection:

Strain Information

Fgb
Name: fibrinogen beta chain
Synonyms: 2510049G14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 110135
HGNC: HGNC:3662
Homologene: 3772
Lamb1
Name: laminin B1
Synonyms: Lamb-1, C81607, C80098, D130003D08Rik, Lamb1-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Ints1
Name: integrator complex subunit 1
Synonyms: 1110015K06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68510
Homologene: 53111
Ube3a
Name: ubiquitin protein ligase E3A
Synonyms: Hpve6a, E6-AP ubiquitin protein ligase, A130086L21Rik, 5830462N02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22215
Homologene: 7988
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, Aspm, D330028K02Rik, MCPH5, Calmbp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12316
Homologene: 7650
Arid2
Name: AT-rich interaction domain 2
Synonyms: 4432409D24Rik, zipzap/p200, 1700124K17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 77044
Homologene: 14601
Hunk
Name: hormonally upregulated Neu-associated kinase
Synonyms: Mak-v, Bstk1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 26559
VEGA: 16
Homologene: 8742
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 87,880,449 bp
  • T to C, chromosome 1 at 139,479,002 bp
  • T to A, chromosome 1 at 150,333,500 bp
  • G to A, chromosome 2 at 24,926,405 bp
  • T to C, chromosome 2 at 53,101,454 bp
  • T to A, chromosome 2 at 102,737,909 bp
  • T to A, chromosome 2 at 144,824,037 bp
  • A to G, chromosome 2 at 153,780,992 bp
  • G to A, chromosome 3 at 38,889,468 bp
  • A to T, chromosome 3 at 83,046,176 bp
  • T to C, chromosome 3 at 135,248,413 bp
  • A to G, chromosome 3 at 153,867,840 bp
  • A to G, chromosome 4 at 144,455,869 bp
  • A to T, chromosome 5 at 74,462,651 bp
  • A to T, chromosome 5 at 96,731,689 bp
  • A to T, chromosome 5 at 109,427,273 bp
  • A to G, chromosome 5 at 139,772,145 bp
  • A to G, chromosome 6 at 18,381,033 bp
  • A to G, chromosome 6 at 52,208,414 bp
  • G to A, chromosome 7 at 28,137,329 bp
  • A to T, chromosome 7 at 59,276,059 bp
  • A to T, chromosome 7 at 119,978,222 bp
  • T to C, chromosome 7 at 140,070,397 bp
  • T to A, chromosome 7 at 140,667,353 bp
  • T to C, chromosome 7 at 140,777,981 bp
  • TCCCTGATGAAC to TC, chromosome 8 at 36,994,229 bp
  • G to T, chromosome 10 at 23,993,362 bp
  • A to T, chromosome 10 at 42,801,681 bp
  • A to G, chromosome 10 at 52,027,563 bp
  • A to C, chromosome 10 at 87,567,347 bp
  • G to T, chromosome 10 at 127,742,688 bp
  • G to C, chromosome 11 at 49,626,686 bp
  • A to G, chromosome 11 at 52,113,187 bp
  • T to C, chromosome 11 at 60,393,821 bp
  • C to T, chromosome 11 at 74,843,078 bp
  • A to G, chromosome 12 at 31,299,931 bp
  • A to T, chromosome 12 at 55,820,766 bp
  • A to T, chromosome 12 at 80,722,366 bp
  • A to C, chromosome 12 at 114,682,907 bp
  • G to T, chromosome 14 at 61,403,014 bp
  • A to G, chromosome 15 at 96,372,205 bp
  • T to C, chromosome 16 at 19,701,317 bp
  • T to G, chromosome 16 at 56,259,820 bp
  • T to A, chromosome 16 at 90,453,739 bp
  • T to A, chromosome 16 at 91,664,987 bp
  • T to C, chromosome 17 at 21,584,816 bp
  • T to A, chromosome 17 at 21,820,002 bp
  • A to G, chromosome 17 at 30,690,261 bp
  • T to A, chromosome 17 at 56,803,587 bp
  • T to C, chromosome 17 at 81,648,961 bp
  • C to A, chromosome 18 at 33,464,305 bp
  • C to T, chromosome 19 at 47,143,703 bp
  • A to T, chromosome 19 at 59,300,540 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5766 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044424-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.