Strain Detail Sheet

Strain Name:
B6.129X1-Abcc8tm1.1Mgn/Mmnc
Stock Number:
011968-UNC
Citation ID:
RRID:MMRRC_011968-UNC

Strain Information

Gene Details

Abcc8tm1.1Mgn
Name: ATP-binding cassette, sub-family C member 8; targeted mutation 1.1, Mark A Magnuson
Synonyms: Sur1neo, SUR1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Targeted Mutation
Abcc8
Name: ATP-binding cassette, sub-family C member 8
Synonyms: SUR1, Sur, D930031B21Rik
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Targeted Mutation
NCBI: 20927
HGNC: HGNC:59
Homologene: 68048
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Genetic Alterations
A gene targeting strategy that involved the use of Cre/loxP was used generate mice that globally lack the proximal promoter and exon 1 of the sulfonylurea receptor type 1 (Sur1) gene. A pgk-neomycin resistance gene remains in the locus.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    RW-4 derived from 129X1/SvJ

    Strain Description

    Phenotype
    Homozygous: Mice that are homozygous null for Sur1 (Abcc8) are viable, fertile and grossly indistinguishable from their wild type littermates except after 16 wks when they become mildly hypoglycemia. Mice lacking Sur1 (Abcc8) lack functional K-ATP channels in pancreatic beta cells but remain euglycemic despite having beta cells that are constantly depolarized due to the lack of this protein.

    Heterozygous:     These mice are also viable and do not differ from wild type.
    Mammalian Phenotype Terms
    Allelic Composition: Abcc8tm1.1Mgn/Abcc8tm1.1Mgn (Genetic Background: involves: 129X1/SvJ * C57BL/6 )
    MeSH Terms
    • ATP-Binding Cassette Transporters
    • Animals
    • Blood Glucose/metabolism
    • Carbachol/pharmacology
    • Cloning, Molecular
    • Eating/physiology
    • Exocytosis
    • Genotype
    • Glucose Clamp Technique
    • Insulin/secretion
    • Islets of Langerhans/drug effects
    • Islets of Langerhans/secretion
    • Mice
    • Mice, Knockout
    • Molecular Sequence Data
    • Perfusion
    • Potassium Channels/deficiency
    • Potassium Channels/genetics
    • Potassium Channels/physiology
    • Potassium Channels, Inwardly Rectifying
    • Receptors, Drug/deficiency
    • Receptors, Drug/genetics
    • Receptors, Drug/physiology
    • Recombinant Proteins/metabolism
    • Sulfonylurea Receptors
    Strain Development
    After achieving germline transmission mice carrying the Sur1neo allele were backcrossed for ten generations.
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Diabetes/Obesity
    • Metabolism
    • Models for Human Disease

    Strain Origin

    Donor
    Mark A. Magnuson, M.D., Vanderbilt University
    Primary Reference
    Shiota C, Larsson O, Shelton KD, Shiota M, Efanov AM, Hoy M, Lindner J, Kooptiwut S, Juntti-Berggren L, Gromada J, Berggren PO, Magnuson MA. Sulfonylurea receptor type 1 knock-out mice have intact feeding-stimulated insulin secretion despite marked impairment in their response to glucose. J Biol Chem. 2002 Oct 4;277(40):37176-83. Epub 2002 Jul 30. (Medline PMID: 12149271)

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    011968-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.