Strain Detail Sheet

Strain Name    :

B6.129X1-Ntrk2tm1Lfr/Mmucd

Stock Number :

000187-UCD

Other Names   :

fBZ, B6.129X1-Ntrk2tm1Lfr/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Target: Ntrk2tm1Lfr
Name: targeted mutation 1, Louis F Reichardt
Alteration at locus: Knock-In
Target: Ntrk2
Name: neurotrophic tyrosine kinase, receptor, type 2
Chromosome: 13
Alteration at locus: Knock-In

Genetic Alterations:
Insertion of trkB kinase cDNA plus polyAloxPtaulacZ into first coding exon of trkB locus. Deletion using cre recombinase results in elimination of trkB expression and turnon of taulacZ expression

Genotype Determination:

ES Cell Line: JM-1

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Floxing results in loss of different populations of neurons depending on pattern of cre expression. Allele results in hypomorphic expression, so mice have severe behavioral abnormalities, including a strong obesity phenotype without floxing.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Ntrk2tm1Lfr/Ntrk2+
        involves: 129X1/SvJ
  • vision/eye phenotype
    • abnormal retinal ganglion cell morphology (MGI Ref ID J:122929)
      • at P28, mice have a greater percent of ON-OFF retinal ganglion cells (RGCs) than in wild-type mice but fewer than in Ntrk2tm1Lfr heterozygotes
  • nervous system phenotype
    • abnormal retinal ganglion cell morphology (MGI Ref ID J:122929)
      • at P28, mice have a greater percent of ON-OFF retinal ganglion cells (RGCs) than in wild-type mice but fewer than in Ntrk2tm1Lfr heterozygotes
Ntrk2tm1Lfr/Ntrk2tm1Lfr
        involves: 129X1/SvJ
  • vision/eye phenotype
    • abnormal retinal ganglion cell morphology (MGI Ref IDs J:119332, J:122929)
      • at P28, mice have a greater percent of ON-OFF retinal ganglion cells (RGCs) than in Ntrk2tm1Lfr heterozygotes and wild-type mice (MGI Ref ID J:122929)
      • at P28, mice have a greater percent of ON-OFF RGCs than wild-type at P13 (MGI Ref ID J:122929)
      • at P28, mice have fewer ON RGCs than wild-type mice (MGI Ref ID J:122929)
      • by P90, the number of retinal ganglion cell axons is reduced by 16% with the greatest effect on myelinated axons (MGI Ref ID J:119332)
      • decreased retinal ganglion cell number (MGI Ref ID J:119332)
        • cells in the retinal ganglion layer are reduced by 20% at 3 months of age
  • nervous system phenotype
    • abnormal retinal ganglion cell morphology (MGI Ref IDs J:119332, J:122929)
      • at P28, mice have a greater percent of ON-OFF retinal ganglion cells (RGCs) than in Ntrk2tm1Lfr heterozygotes and wild-type mice (MGI Ref ID J:122929)
      • at P28, mice have a greater percent of ON-OFF RGCs than wild-type at P13 (MGI Ref ID J:122929)
      • at P28, mice have fewer ON RGCs than wild-type mice (MGI Ref ID J:122929)
      • by P90, the number of retinal ganglion cell axons is reduced by 16% with the greatest effect on myelinated axons (MGI Ref ID J:119332)
      • decreased retinal ganglion cell number (MGI Ref ID J:119332)
        • cells in the retinal ganglion layer are reduced by 20% at 3 months of age

Strain of Origin: mixed 129 x C57BL/6

Strain genetic background: C57BL/6 Charles River

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Neurobiology
  • Research Tools

Strain Origin

Donor: Louis F. Reichardt, Ph.D., University of California - San Francisco

Primary Reference:

  • Xu B, Zang K, Ruff NL, Zhang YA, McConnell SK, Stryker MP, Reichardt LF. Cortical degeneration in the absence of neurotrophin signaling: dendritic retraction and neuronal loss after removal of the receptor TrkB. Neuron. 2000 Apr;26(1):233-45. (Medline PMID: 10798407)
  • Xu B, Gottschalk W, Chow A, Wilson RI, Schnell E, Zang K, Wang D, Nicoll RA, Lu B, Reichardt LF. The role of brain-derived neurotrophic factor receptors in the mature hippocampus: modulation of long-term potentiation through a presynaptic mechanism involving TrkB. J Neurosci. 2000 Sep 15;20(18):6888-97. (Medline PMID: 10995833)
  • Rico B, Xu B, Reichardt LF. TrkB receptor signaling is required for establishment of GABAergic synapses in the cerebellum. Nat Neurosci. 2002 Mar;5(3):225-33. (Medline PMID: 11836532)

Colony and Husbandry Information

Special Considerations

Mice should be bred before 4 months of age. Homozygous mice develop early onset obesity and may need teeth trimming.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • Heterozygous female x C57BL/6 male
  • C57BL/6 female x Heterozygous male

Generation: ~N10

Overall Breeding Performance: Poor

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 16 weeks 16 weeks

Average litter size: 6

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000187-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
000187-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
000187-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/17/2014