Strain Name:
B6N.129X1-Ntrk2tm1Lfr/Mmucd
Stock Number:
000187-UCD
Citation ID:
RRID:MMRRC_000187-UCD
Other Names:
fBZ

Gene Information

Ntrk2tm1Lfr
Name: neurotrophic tyrosine kinase, receptor, type 2; targeted mutation 1, Louis F Reichardt
Synonyms: trkBfbz, fBZ, TrkBlox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knock-In
Ntrk2
Name: neurotrophic tyrosine kinase, receptor, type 2
Synonyms: Tkrb, C030027L06Rik, trkB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knock-In
NCBI: 18212
VEGA: 13
HGNC: HGNC:8032
Homologene: 4504
Genetic Alterations
Insertion of Ntrk2 (TrkB) kinase cDNA plus polyA-loxP-tau-lacZ into first coding exon of the TrkB locus. Deletion using Cre recombinase results in the elimination of trkB expression and turn on of tau-lacZ expression
Genotype Determination
ES Cell Line
JM-1 derived from 129X1/SvJ
Phenotype
Floxing results in loss of different populations of neurons depending on the pattern of Cre expression. Allele results in hypomorphic expression, so mice have severe behavioral abnormalities, including a strong obesity phenotype without floxing.
Mammalian Phenotype Terms
Allelic Composition: Ntrk2tm1Lfr/Ntrk2tm1Lfr (Genetic Background: involves: 129X1/SvJ )

Allelic Composition: Ntrk2tm1Lfr/Ntrk2+ (Genetic Background: involves: 129X1/SvJ )

MeSH Terms
  • Animals
  • Cell Count
  • DNA-Binding Proteins/metabolism
  • Dendrites/metabolism
  • Dendrites/pathology
  • Mice
  • Mice, Transgenic
  • Mutation/genetics
  • Neocortex/metabolism
  • Neocortex/pathology
  • Nerve Growth Factors/metabolism
  • Neurodegenerative Diseases/metabolism
  • Neurodegenerative Diseases/pathology
  • Neurons/metabolism
  • Neurons/pathology
  • Pyramidal Cells/metabolism
  • Receptor, trkB/genetics
  • Receptor, trkB/metabolism
  • Transcription Factors/metabolism
  • beta-Galactosidase/metabolism
  • Antigens, Differentiation/metabolism
  • Axons/metabolism
  • Brain-Derived Neurotrophic Factor/metabolism
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Calcium-Calmodulin-Dependent Protein Kinases/genetics
  • Hippocampus/cytology
  • Hippocampus/metabolism
  • In Vitro Techniques
  • Long-Term Potentiation/genetics
  • Mice, Inbred C57BL
  • Neuronal Plasticity/genetics
  • Patch-Clamp Techniques
  • Presynaptic Terminals/metabolism
  • RNA, Messenger/biosynthesis
  • Receptor, Nerve Growth Factor/antagonists & inhibitors
  • Receptor, Nerve Growth Factor/metabolism
  • Receptor, trkB/deficiency
  • Receptors, Glutamate/metabolism
  • Signal Transduction/genetics
  • Stem Cells
  • Axons/physiology
  • Carrier Proteins/metabolism
  • Cerebellum/cytology
  • Cerebellum/metabolism
  • GABA Plasma Membrane Transport Proteins
  • Genes, Reporter
  • Glutamate Decarboxylase/metabolism
  • In Situ Hybridization
  • Integrases/genetics
  • Integrases/metabolism
  • Interneurons/cytology
  • Interneurons/metabolism
  • Isoenzymes/metabolism
  • Membrane Proteins/metabolism
  • Membrane Transport Proteins
  • Neurons/ultrastructure
  • Organic Anion Transporters
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/metabolism
  • Purkinje Cells/cytology
  • Purkinje Cells/metabolism
  • Recombination, Genetic
  • Signal Transduction
  • Synapses/physiology
  • Viral Proteins/genetics
  • Viral Proteins/metabolism
  • Wnt Proteins
  • Wnt1 Protein
  • Zebrafish Proteins
  • gamma-Aminobutyric Acid/metabolism
Strain Development
Targeting in JM-1 embryonic stem cells (129X1/SvJ) followed by 10 backcrosses to C57BL/6NCrl.
Suggested Control Mice
Wild-type littermates
  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Neurobiology
  • Research Tools
Donor
Louis F. Reichardt, Ph.D.,  University of California, San Francisco
Primary Reference

Xu B, Zang K, Ruff NL, Zhang YA, McConnell SK, Stryker MP, Reichardt LF. Cortical degeneration in the absence of neurotrophin signaling: dendritic retraction and neuronal loss after removal of the receptor TrkB. Neuron. 2000 Apr;26(1):233-45. (Medline PMID: 10798407)

Xu B, Gottschalk W, Chow A, Wilson RI, Schnell E, Zang K, Wang D, Nicoll RA, Lu B, Reichardt LF. The role of brain-derived neurotrophic factor receptors in the mature hippocampus: modulation of long-term potentiation through a presynaptic mechanism involving TrkB. J Neurosci. 2000 Sep 15;20(18):6888-97. (Medline PMID: 10995833)

Rico B, Xu B, Reichardt LF. TrkB receptor signaling is required for establishment of GABAergic synapses in the cerebellum. Nat Neurosci. 2002 Mar;5(3):225-33. (Medline PMID: 11836532)

Colony and Husbandry Information

Mice should be bred before 4 months of age. Homozygous mice develop early onset obesity and may need teeth trimming.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • Heterozygous female x C57BL/6 male
  • C57BL/6 female x Heterozygous male
Generation
N10 (C57BL/6NCrl)
Overall Breeding Performance
Poor
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
6
Recommended wean age
3 weeks
Average Pups Weaned
8

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
000187-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
000187-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2
000187-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.