Strain Name    : JU.Cg-Ay/a/Mmmh

Stock Number : 000221-MU

Gene Information

(provided by MGI)
Allele Symbol: Ay
Name: agouti yellow
Chromosome: 2
Alteration at locus: Spontaneous Mutation
Genetic Alterations:
The mutation appears to be a DNA structural alteration that disrupts a gene, hnRNP associated with lethal yellow (Raly), 5' to the agouti locus and places the agouti locus under the control of the Raly promotor.

Genotype Determination:

ES Cell Line:

Phenotype

The mutant allele is characterized by yellow color, obesity, and diabetes in heterozygotes. It is prenatal lethal in the homozygous state. Therefore, black offspring will be produced every generation. In mice mutant at the agouti locus, these phenotypes are all reduced if the phenotype of the mouse is more eumelanic than pheomelanic,as is the case when the same mutant allele is backcrossed onto the JU/CtLm (MMRRC:000136) inbred background. The mice are yellow until the first molt, then become umbrous (darker dorsally). After about 6 months of age, this umbrous phenotype is gradually lost.

Mammalian Phenotype Terms:
Allelic Composition: Ay/Ay (Genetic Background: 101-Ay )

  • mortality/aging
  • other phenotype
Allelic Composition: Ay/Aw (Genetic Background: 129S1.C3-Ay/Aw Chr 19MOLF/Ei )

Allelic Composition: Ay/a (Genetic Background: B6.Cg-Ay/J )

Allelic Composition: Ay/A (Genetic Background: C3HeB/FeJ )

Allelic Composition: Ay/A (Genetic Background: involves: C57BL/6 )

Allelic Composition: Ay/a (Genetic Background: involves: KK )

Strain of Origin: old mutant of the mouse fancy

Strain genetic background: JU/CtLm (MMRRC:000136)

Strain Development: Spontaneous mutation maintained by backcross to wildtype JU/CtLm (MMRRC:000136) mice.

Suggested Control Mice:

  • Pigmentation
  • Diabetes/Obesity
  • Reproduction

Donor: Dr. M. L. Lamoreux, Texas A&M University

Primary Reference:

  • Michaud EJ, Bultman SJ, Klebig ML, van Vugt MJ, Stubbs LJ, Russell LB, Woychik RP. A molecular model for the genetic and phenotypic characteristics of the mouse lethal yellow (Ay) mutation. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2562-6. (Medline: 8146154)
  • Lamoreux ML, Wakamatsu K, Ito S. Interaction of major coat color gene functions in mice as studied by chemical analysis of eumelanin and pheomelanin. Pigment Cell Res. 2001 Feb;14(1):23-31. (Medline: 11277491)
  • Ollmann MM, Lamoreux ML, Wilson BD, Barsh GS. Interaction of Agouti protein with the melanocortin 1 receptor in vitro and in vivo. Genes Dev. 1998 Feb 1;12(3):316-30. (Medline: 9450927)

Colony and Husbandry Information

Feed mouse chow that contains 4 percent fat.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Coat color: Yellow

Other:

MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • JU/CtLm Wildtype Female x Heterozygous Yellow Male
  • Heterozygous Yellow Female x JU/CtLm Wildtype Male
  • Heterozygous Yellow Female x Heterozygous Yellow Male

Generation: N10+

Overall Breeding Performance: Good for young mice but quickly drops off as mice age. Both males and females quickly become sterile if they become obese.

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 20 weeks 20 weeks

Average litter size: Unknown

Recommended wean age: 4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
000221-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
000221-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
000221-MU-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.