Strain Detail Sheet

Strain Name    :

JU.Cg-Ay/a/Mmmh

Stock Number :

000221-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Ay
Name: agouti yellow
Chromosome: 2
Alteration at locus: Spontaneous Mutation

Genetic Alterations:
The mutation appears to be a DNA structural alteration that disrupts a gene, hnRNP associated with lethal yellow (Raly), 5' to the agouti locus and places the agouti locus under the control of the Raly promotor.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

The mutant allele is characterized by yellow color, obesity, and diabetes in heterozygotes. It is prenatal lethal in the homozygous state. Therefore, black offspring will be produced every generation. In mice mutant at the agouti locus, these phenotypes are all reduced if the phenotype of the mouse is more eumelanic than pheomelanic,as is the case when the same mutant allele is backcrossed onto the JU/CtLm (MMRRC:000136) inbred background. The mice are yellow until the first molt, then become umbrous (darker dorsally). After about 6 months of age, this umbrous phenotype is gradually lost.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Ay/A
        involves: C57BL/6
  • adipose tissue phenotype
    • increased brown adipose tissue amount (MGI Ref ID J:131039)
      • female mice have a 6-fold gain in weight of BAT compared to littermate controls
    • increased gonadal fat pad weight (MGI Ref ID J:131039)
      • female mice have almost a 7-fold gain in weight of fat pad
    • increased inguinal fat pad weight (MGI Ref ID J:131039)
      • female mice have a greater than 6-fold gain in weight of fat pad
    • increased percent body fat (MGI Ref ID J:131039)
      • female mice have almost a 4-fold gain in percentage of body fat compared to littermate controls
      • male mice have almost a 1.8-fold gain in percentage of body fat compared to littermate controls
    • increased retroperitoneal fat pad weight (MGI Ref ID J:131039)
      • female mice have almost a 6-fold gain in weight of fat pad
  • behavior/neurological phenotype
    • hypoactivity (MGI Ref ID J:131039)
      • the locomotor activity of mice is about half that of wild-type mice
    • increased eating behavior (MGI Ref ID J:131039)
      • 18 week old mice eat about 1.4 times more food during a 7 day period compared to wild-type controls
  • growth/size phenotype
    • increased body length (MGI Ref ID J:131039)
      • female but not male mice have a significant 5% increase in their body length
    • increased susceptibility to weight gain (MGI Ref ID J:131039)
      • mice gain weight at a greater rate than littermate controls starting at six weeks of age
    • obese (MGI Ref ID J:131039)
      • by 23 weeks of age, female mice have almost double the weight compared to their wild-type littermate controls
  • homeostasis/metabolism phenotype
    • increased circulating leptin level (MGI Ref ID J:131039)
      • circulating levels of leptin are over 12-fold higher compared to littermate controls
  • liver/biliary system phenotype
    • increased liver weight (MGI Ref ID J:131039)
      • female mice have almost a 2-fold gain in weight of the liver
  • pigmentation phenotype
    • yellow coat color (MGI Ref ID J:131039)
      • mice have a yellow coat color
  • integument phenotype
    • yellow coat color (MGI Ref ID J:131039)
      • mice have a yellow coat color
Ay/a
        B6.Cg-A<y>/J
  • behavior/neurological phenotype
    • abnormal food intake (MGI Ref ID J:102986)
      • the stress of isolation, restraint, or ip injection inhibits feeding
  • pigmentation phenotype
    • abnormal hair follicle melanogenesis (MGI Ref ID J:1295)
      • tyrosinase levels in hairbulb melanocytes ,as determined by 35S methionine incorporation and immunotitration, are reduced in comparison to a/a controls
      • decreased tyrosinase suggests the production of predominantly phaeomelanin (yellow)
  • integument phenotype
    • abnormal hair follicle melanogenesis (MGI Ref ID J:1295)
      • tyrosinase levels in hairbulb melanocytes ,as determined by 35S methionine incorporation and immunotitration, are reduced in comparison to a/a controls
      • decreased tyrosinase suggests the production of predominantly phaeomelanin (yellow)
Ay/a
        involves: KK
  • homeostasis/metabolism phenotype
    • abnormal lipid homeostasis (MGI Ref ID J:26460)
      • lipogenesis from acetate is elevated at 5 weeks of age, the enhanced activity is maintained until 16 weeks of age
      • the acetate/glucose ratio is higher than control in young mice
    • hyperglycemia (MGI Ref ID J:26460)
      • blood glucose levels increase with age in both sexes
      • marked hyperglycemia (400-500 mg/dl) develops by 16 weeks of age
    • impaired glucose tolerance (MGI Ref ID J:26460)
    • increased circulating insulin level (MGI Ref ID J:26460)
      • markedly elevated plasma immunoreactive insulin (IRI) level increases with age
    • increased urine glucose level (MGI Ref ID J:26460)
      • present at all ages tested (5, 10, 16 weeks) and in both sexes
    • insulin resistance (MGI Ref ID J:26460)
      • insulin sensitivity is impaired at 10 weeks an d lost by 16 weeks
  • adipose tissue phenotype
    • increased total body fat amount (MGI Ref ID J:26460)
      • adipose tissue weight increases with age, reaching a maximum at 10 weeks of age
  • growth/size phenotype
    • increased susceptibility to weight gain (MGI Ref ID J:26460)
      • greater body weight gain occurs in females as compared to black KK controls
  • endocrine/exocrine gland phenotype
    • abnormal pancreatic islet morphology (MGI Ref ID J:26460)
      • islets are hypertrophic in 10-16 week old mice
      • central cavity formation with occasional red blood cells is observed in islets
      • degranulated pancreatic beta cells (MGI Ref ID J:26460)
        • beta cells are degranulated
        • degranulated islets are infiltrated with fine glycogen granules
  • renal/urinary system phenotype
    • abnormal renal glomerular capsule (MGI Ref ID J:26460)
      • basement membrane of Bowman

Strain of Origin: old mutant of the mouse fancy

Strain genetic background: JU/CtLm (MMRRC:000136)

Strain Development: Spontaneous mutation maintained by backcross to wildtype JU/CtLm (MMRRC:000136) mice.

Suggested Control Mice:

Research Applications

  • Pigmentation
  • Diabetes/Obesity
  • Reproduction

Strain Origin

Donor: Dr. M. L. Lamoreux, Texas A&M University

Primary Reference:

  • Michaud EJ, Bultman SJ, Klebig ML, van Vugt MJ, Stubbs LJ, Russell LB, Woychik RP. A molecular model for the genetic and phenotypic characteristics of the mouse lethal yellow (Ay) mutation. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2562-6. (Medline: 8146154)
  • Lamoreux ML, Wakamatsu K, Ito S. Interaction of major coat color gene functions in mice as studied by chemical analysis of eumelanin and pheomelanin. Pigment Cell Res. 2001 Feb;14(1):23-31. (Medline: 11277491)
  • Ollmann MM, Lamoreux ML, Wilson BD, Barsh GS. Interaction of Agouti protein with the melanocortin 1 receptor in vitro and in vivo. Genes Dev. 1998 Feb 1;12(3):316-30. (Medline: 9450927)

Colony and Husbandry Information

Special Considerations

Feed mouse chow that contains 4 percent fat.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color: Yellow

Other:

Breeding

MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • JU/CtLm Wildtype Female x Heterozygous Yellow Male
  • Heterozygous Yellow Female x JU/CtLm Wildtype Male
  • Heterozygous Yellow Female x Heterozygous Yellow Male

Generation: N10+

Overall Breeding Performance: Good for young mice but quickly drops off as mice age. Both males and females quickly become sterile if they become obese.

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 20 weeks 20 weeks

Average litter size: Unknown

Recommended wean age: 4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000221-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
000221-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
000221-MU-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

Generated: 04/23/2014