Strain Name:
C.129S4-Ctla4tm1Shr Cd28tm1Shr/Mmnc
Stock Number:
Citation ID:

Gene Information

Name: cytotoxic T-lymphocyte-associated protein 4; targeted mutation 1, Arlene H Sharpe
Synonyms: Ctla4-, CTLA-4-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Name: CD28 antigen; targeted mutation 1, Arlene H Sharpe
Synonyms: Cd28-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Name: CD28 antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
NCBI: 12487
Homologene: 4473
Name: cytotoxic T-lymphocyte-associated protein 4
Synonyms: Cd152, Ctla-4, Ly-56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
NCBI: 12477
Homologene: 3820
Genetic Alterations
Neo cassette inserted into exon 2 of ctla-4 and hygro cassette inserted into exon 2 of cd28.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    J1 derived from 129S4/SvJae
    Homozygous: 1) Defective T cell activation 2) Can also be used to study B7-1: PD-L1 interactions

    Heterozygous: Same as wild-type.
    Mammalian Phenotype Terms
    Allelic Composition: Ctla4tm1Shr/Ctla4tm1Shr (Genetic Background: B6.129S4-Ctla4tm1Shr )

    Allelic Composition: Ctla4tm1Shr/Ctla4tm1Shr (Genetic Background: C.129S4-Ctla4tm1Shr )

    Allelic Composition: Ctla4tm1Shr/Ctla4tm1Shr (Genetic Background: either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6) )

    Allelic Composition: Cd28tm1Shr/Cd28tm1Shr (Genetic Background: involves: 129S4/SvJae * BALB/c * C57BL/6 )

    MeSH Terms
    • Abatacept
    • Animals
    • Antigens, CD/genetics
    • Antigens, CD/immunology
    • Antigens, CD/physiology
    • Antigens, Differentiation/genetics
    • Antigens, Differentiation/immunology
    • B7-1 Antigen/genetics
    • B7-1 Antigen/immunology
    • B7-1 Antigen/physiology
    • B7-2 Antigen
    • CD28 Antigens/genetics
    • CD28 Antigens/immunology
    • CHO Cells
    • CTLA-4 Antigen
    • Cell Division
    • Cricetinae
    • Graft Rejection/immunology
    • Graft Survival/immunology
    • Heart Transplantation
    • Immunoconjugates
    • Immunophenotyping
    • Interferon-gamma/biosynthesis
    • Membrane Glycoproteins/genetics
    • Membrane Glycoproteins/immunology
    • Membrane Glycoproteins/physiology
    • Mice
    • Mice, Inbred BALB C
    • Mice, Inbred C57BL
    • Mice, Knockout
    • T-Lymphocytes/cytology
    • T-Lymphocytes/immunology
    • Th1 Cells/immunology
    • Transplantation, Homologous/immunology
    • B7-1 Antigen/metabolism
    • B7-H1 Antigen
    • COS Cells
    • Cell Line
    • Cell Proliferation
    • Cells, Cultured
    • Cercopithecus aethiops
    • Cytokines/antagonists & inhibitors
    • Cytokines/biosynthesis
    • Down-Regulation/genetics
    • Down-Regulation/immunology
    • Humans
    • Ligands
    • Membrane Glycoproteins/deficiency
    • Membrane Glycoproteins/metabolism
    • Peptides/deficiency
    • Peptides/metabolism
    • Peptides/physiology
    • Protein Binding/genetics
    • Protein Binding/immunology
    • T-Lymphocytes/metabolism
    Strain Development
    129 CD28.CTLA-4 deficient strain crossed with WT BALB/c mice, then heterozygotes crosseed with WT BALB/c mice for 9 generations and subsequently intercrosseed and bred to homozygosites.
    Suggested Control Mice

    Wildtype littermates

    • Immunology and Inflammation
    Arlene H. Sharpe, M.D., Ph.D., Harvard Medical School
    Primary Reference
    • B7-dependent T-cell costimulation in mice lacking CD28 and CTLA4. Mandelbrot DA, Oosterwegel MA, Shimizu K, Yamada A, Freeman GJ, Mitchell RN, Sayegh MH, Sharpe AH. J Clin Invest. 2001 Apr;107(7):881-7. (Medline PMID: 11285307)
    • Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses. Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ. Immunity. 2007 Jul;27(1):111-22. Epub 2007 Jul 12. (Medline PMID: 17629517)

    Colony and Husbandry Information

    Immunodeficient strain, so may want to use acidified water or antibiotics.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    030493-UNC-SPERM Cryo-preserved spermatozoa $548.00 / Non-Profit Aliquot Approximate quantity2
    030493-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to for more information.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.