Strain Detail Sheet

Strain Name    :

129S4-Chrna10tm1Bedv/Mmucd

Stock Number :

030509-UCD

Other Names   :

Alpha 10 KO, 129S4-Chrna10tm1Bedv/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Chrna10tm1Jbou
Name: targeted mutation 1, Jim Boulter
Alteration at locus: Knockout
Gene Symbol: Chrna10
Name: cholinergic receptor, nicotinic, alpha polypeptide 10
Chromosome: 7
Alteration at locus: Knockout

Genetic Alterations:
A portion of Chrna10 exon 3 was deleted and replaced with a 'floxed' PGKneo cassette. The PGKneo cassette was subsequently removed by mating Chrna10+/- females to transgenic protamine cre male mice.

Genotype Determination:

ES Cell Line: R1 derived from 129S4

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: In Chrna10 null mutant mice, olivocochlear fiber stimulation fails to suppress distortion product otoacoustic emissions. In addition, Chrna10-/- mice exhibit abnormal olivocochlear morphology and disorganized efferent innervation to inner and outer hair cells.

Heterozygous phenotype: No known phenotype associated with heterozygous Chrna10+/- mice.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Chrna10tm1Jbou/Chrna10tm1Jbou
        Background Not Specified
  • hearing/vestibular/ear phenotype
    • hearing/vestibular/ear phenotype (MGI Ref ID J:130585)
      • no differences in baseline auditory brainstem responses (ABRs), summed activity of auditory neurons evoked by short tone pips or in distortion product autoacoustic emissions (DPOAEs)
      • abnormal cochlear hair cell morphology (MGI Ref ID J:130585)
        • abnormal cochlear IHC efferent innervation pattern (MGI Ref ID J:130585)
          • the inner spiral bundle appears disorganized
          • modiolar-side terminals are larger than controls
          • fewer terminals on the pillar side of the hair cell
        • abnormal cochlear OHC efferent innervation pattern (MGI Ref ID J:130585)
          • efferent terminals contacting OHCs are larger in size but fewer in number than controls
          • 50% of OHCs had more than one synaptic terminal
      • abnormal cochlear hair cell physiology (MGI Ref ID J:130585)
        • abnormal cochlear inner hair cell physiology (MGI Ref ID J:130585)
          • both inward and outward currents elicited by the application of acetycholinesterase are not detectable
          • synaptic currents elicited by the superfusion of KCl to depolarize synaptic terminals are not detectable
        • abnormal cochlear outer hair cell physiology (MGI Ref ID J:130585)
          • inward currents elicited by the application o f acetycholinesterase are either not detectable or reduced
          • outward currents elicited by the application of acetycholinesterase are either not detectable or reduced
          • synaptic currents elicited by the superfusion of KCl to depolarize synaptic terminals are not detectable
          • small inward currents are detected when both KCl and acetylcholinesterase are applied; nicotine did not evoke currents in OHCs that were responsive to ACh
      • abnormal distortion product otoacoustic emission (MGI Ref ID J:130585)
        • a during-shocks enhancement was observed; after shock-train offset, amplitudes and offset time constants were similar to controls
        • efferent-evoked supression of DPOAEs was never observed
  • nervous system phenotype
    • abnormal cochlear hair cell morphology (MGI Ref ID J:130585)
      • abnormal cochlear IHC efferent innervation pattern (MGI Ref ID J:130585)
        • the inner spiral bundle appears disorganized
        • modiolar-side terminals are larger than controls
        • fewer terminals on the pillar side of the hair cell
      • abnormal cochlear OHC efferent innervation pattern (MGI Ref ID J:130585)
        • efferent terminals contacting OHCs are larger in size but fewer in number than controls
        • 50% of OHCs had more than one synaptic terminal
    • abnormal cochlear hair cell physiology (MGI Ref ID J:130585)
      • abnormal cochlear inner hair cell physiology (MGI Ref ID J:130585)
        • both inward and outward currents elicited by the application of acetycholinesterase are not detectable
        • synaptic currents elicited by the superfusion of KCl to depolarize synaptic terminals are not detectable
      • abnormal cochlear outer hair cell physiology (MGI Ref ID J:130585)
        • inward currents elicited by the application of acetychol inesterase are either not detectable or reduced
        • outward currents elicited by the application of acetycholinesterase are either not detectable or reduced
        • synaptic currents elicited by the superfusion of KCl to depolarize synaptic terminals are not detectable
        • small inward currents are detected when both KCl and acetylcholinesterase are applied; nicotine did not evoke currents in OHCs that were responsive to ACh

Strain of Origin: 129X1/SvJ

Strain genetic background: 129S4/SvJae

Strain Development: Founder male mouse mated to female 129S4/SvJae. Continuous backcrossing of heterozygous progeny (both male and female) with wild type 129S4/SvJae mice through N11.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Neurobiology
  • Research Tools
  • Sensorineural
  • Auditory physiology

Strain Origin

Donor: Jim Boulter, Ph.D., UCLA

Primary Reference:

  • Vetter DE, Katz E, Maison SF, Taranda J, Turcan S, Ballestero J, Liberman MC, Elgoyhen AB, Boulter J. The alpha10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system. Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20594-9. Epub 2007 Dec 12. (Medline PMID: 18077337 )
  • Elgoyhen AB, Vetter DE, Katz E, Rothlin CV, Heinemann SF, Boulter J. alpha10: a determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3501-6. Epub 2001 Mar 6. (Medline PMID: 11248107 )

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030509-UCD-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
030509-UCD-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
030509-UCD-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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