Strain Name:
129S4-Chrna10tm1Jbou/Mmucd
Stock Number:
030509-UCD
Citation ID:
RRID:MMRRC_030509-UCD
Other Names:
Alpha 10 KO, 129S4-Chrna10tm1Bedv/Mmcd

Strain Information

Chrna10tm1Jbou
Name: cholinergic receptor, nicotinic, alpha polypeptide 10; targeted mutation 1, Jim Boulter
Synonyms: alpha10-, Chrna10tm1Bedv
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Chrna10
Name: cholinergic receptor, nicotinic, alpha polypeptide 10
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 504186
Homologene: 56886
Genetic Alterations
A portion of Chrna10 exon 3 was deleted and replaced with a 'floxed' PGKneo cassette. The PGKneo cassette was subsequently removed by mating Chrna10+/- females to transgenic protamine cre male mice.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: In Chrna10 null mutant mice, olivocochlear fiber stimulation fails to suppress distortion product otoacoustic emissions. In addition, Chrna10-/- mice exhibit abnormal olivocochlear morphology and disorganized efferent innervation to inner and outer hair cells.

Heterozygous: No known phenotype associated with heterozygous Chrna10+/- mice.
Mammalian Phenotype Terms
Allelic Composition: Chrna10tm1Jbou/Chrna10tm1Jbou (Genetic Background: Not Specified )

MeSH Terms
  • Animals
  • Cochlea/cytology
  • Cochlea/physiology
  • Electric Stimulation
  • Electrophysiology
  • Hair Cells, Auditory/physiology
  • Mice
  • Mice, Knockout
  • Olivary Nucleus/physiology
  • Receptors, Nicotinic/genetics
  • Receptors, Nicotinic/physiology
  • Synapses/physiology
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Female
  • Gene Expression
  • Hair Cells, Auditory/metabolism
  • Hair Cells, Vestibular/metabolism
  • Hair Cells, Vestibular/physiology
  • Humans
  • Molecular Sequence Data
  • Rats
  • Vestibule, Labyrinth/cytology
  • Xenopus laevis
Strain Development
Founder male mouse mated to female 129S4/SvJae. Continuous backcrossing of heterozygous progeny (both male and female) with wild type 129S4/SvJae mice through N11.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Neurobiology
  • Research Tools
  • Sensorineural
  • Auditory physiology
Donor
Jim Boulter, Ph.D., University of California, Los Angeles
Primary Reference
  • Vetter DE, Katz E, Maison SF, Taranda J, Turcan S, Ballestero J, Liberman MC, Elgoyhen AB, Boulter J. The alpha10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system. Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20594-9. Epub 2007 Dec 12. (Medline PMID: 18077337)
  • Elgoyhen AB, Vetter DE, Katz E, Rothlin CV, Heinemann SF, Boulter J. alpha10: a determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3501-6. Epub 2001 Mar 6. (Medline PMID: 11248107)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030509-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
030509-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
030509-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.