Allele Symbol: Chrna10tm1Jbou
Name: targeted mutation 1, Jim Boulter
Alteration at locus: Knockout
Gene Symbol: Chrna10
Name: cholinergic receptor, nicotinic, alpha polypeptide 10
Alteration at locus: Knockout
ES Cell Line: R1 derived from 129S4
Homozygous phenotype: In Chrna10 null mutant mice, olivocochlear fiber stimulation fails to suppress distortion product otoacoustic emissions. In addition, Chrna10-/- mice exhibit abnormal olivocochlear morphology and disorganized efferent innervation to inner and outer hair cells.
Heterozygous phenotype: No known phenotype associated with heterozygous Chrna10+/- mice.
Strain of Origin: 129X1/SvJ
Strain genetic background: 129S4/SvJae
Strain Development: Founder male mouse mated to female 129S4/SvJae. Continuous backcrossing of heterozygous progeny (both male and female) with wild type 129S4/SvJae mice through N11.
Suggested Control Mice: Wild-type littermates
Donor: Jim Boulter, Ph.D., UCLA
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.