Strain Detail Sheet

Strain Name    :

B6;CBA-Tg(ATP6V1B1-EGFP)1Rnel/Mmjax

Stock Number :

032047-JAX

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(ATP6V1B1-EGFP)1Rnel
Name: transgene insertion 1, Raoul Nelson
Alteration at locus: Transgenic
Reporter: EGFP (Jelly Fish)
Name: Enhanced Green Fluorescent Protein
Alteration at locus: Transgenic
Transgene: ATP6V1B1 (Human)
Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Alteration at locus: Transgenic

Genetic Alterations:

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Not described.

Hemizygous phenotype: Mice hemizygous for this B1-EGFP transgene are viable and fertile, with expression of enhanced green fluorescent protein (EGFP) directed to epithelial cells of the kidney, epididymis, and lung by the human V-ATPase B1 subunit (ATP6V1B1) promoter region. Specifically, EGFP is expressed at high levels in renal type A and type B intercalated cells, all epididymal narrow and clear cells, and some nonciliated airway epithelial cells. While no EGFP expression is detected in collecting duct principal cells or epididymal principal cells, about half of the renal connecting tubule principal cells show EGFP expression.

Strain of Origin: (C57BL/6xCBA)F1

Strain genetic background: C57BL/6 x CBA

Strain Development: The B1-EGFP transgene was designed with ~7 kb sequence from the 5' flanking region of the human V-ATPase B1 subunit (ATP6V1B1) gene (extending to but excluding the endogenous translational start codon) upstream of an enhanced green fluorescent protein (EGFP) coding region, and all followed by an SV40 early region polyadenylation signal. This transgene was injected into the pronuclei of single-cell (C57BL/6xCBA)F1 embryos. The resulting transgenic offspring, founder mouse F1 and F16, were found to have at least 100 copies per cell equivalent of the transgene. Mouse F1 and F16 were bred to (C57BL/6xCBA)F1 mice to establish founder line 1 and line 3, respectively. These B1-EGFP transgenic mice were then maintained on a mixed C57BL/6;CBA genetic background by breeding transgenic mice with wildtype mice for many generations prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred with B6CBAF1/J hybrid mice for at least one generation to establish the colony.

Suggested Control Mice: Wild-type littermates

Research Applications

    Strain Origin

    Donor: Raoul Nelson, M.D., University of Utah School of Medicine

    Primary Reference:

    Miller RL; Zhang P; Smith M; Beaulieu V; Paunescu TG; Brown D; Breton S; Nelson RD, V-ATPase B1-subunit promoter drives expression of EGFP in intercalated cells of kidney, clear cells of epididymis and airway cells of lung in transgenic mice., Am J Physiol Cell Physiol 2005 May;288(5):C1134-44 (Medline PMID: 15634743)

    Colony and Husbandry Information

    Special Considerations

    When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to B6CBAF1/J hybrid mice.

    Health Status Report

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    The donor or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    - Products for this strain are Not Yet Available for Ordering -
    If you register interest in this strain, you will be notified when it becomes available for ordering.
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    032047-JAX-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    032047-JAX-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    This strain is not currently available. If you register interest in this strain, you will be notified when it becomes available for ordering (click Register Interest button above).



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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    Generated: 04/24/2014