Strain Name    : FVB.129S4(B6)-Ptpn1tm1Bbk/Mmjax

Stock Number : 032242-JAX

Gene Information

(provided by MGI)
Allele Symbol: Ptpn1tm1Bbk
Name: targeted mutation 1, Barabra B Kahn
Chromosome: 2
Alteration at locus: Knockout
Gene Symbol: Ptpn1
Name: protein tyrosine phosphatase, non-receptor type 1
Chromosome: 2
Alteration at locus: Knockout
Genetic Alterations:
A neomycin cassette was inserted into exon 1.

Genotype Determination:

ES Cell Line: J1 derived from 129S4/SvJae

Phenotype

Homozygous: Decreased adiposity and insulin sensitivity; resistant to high fat diet-induced obesity.

Heterozygous: normal

Mammalian Phenotype Terms:
Allelic Composition: Ptpn1tm1Bbk/Ptpn1tm1Bbk (Genetic Background: involves: 129/Sv * C57BL/6 )

Allelic Composition: Ptpn1tm1Bbk/Ptpn1tm1Bbk (Genetic Background: involves: 129S4/SvJae * C57BL/6J )

Allelic Composition: Ptpn1tm1Bbk/Ptpn1+ (Genetic Background: involves: 129S4/SvJae * C57BL/6J )

Strain of Origin: 129S4/SvJae

Strain genetic background: FVB.129S4

Strain Development: The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J.  The rusulting hybrids was then backcrossed to FVB/N for 20 generations.

Suggested Control Mice: Wild-type littermates or FVB/N mice

  • Diabetes/Obesity
  • Metabolism

Donor: Benjamin G. Neel, M.D., Ph.D., Ontario Cancer Institute.

Primary Reference:

Klaman LD, Boss O, Peroni OD, Kim JK, Martino JL, Zabolotny JM, Moghal N, Lubkin M, Kim YB, Sharpe AH, Stricker-Krongrad A, Shulman GI, Neel BG, Kahn BB. Increased energy expenditure, decreased adiposity, and tissue-specific insulin sensitivity in protein-tyrosine phosphatase 1B-deficient mice. Mol Cell Biol. 2000 Aug;20(15):5479-89. (Medline PMID: 10891488)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Coat color: White (albino)

Other:

MMRRC Breeding System: Backcross or Sib-mating
While maintaining a live colony, these mice are bred as homozygotes.

Breeding Scheme(s):

    Generation: N20+

    Overall Breeding Performance: Excellent

    Viability and Fertility:FemaleMaleComments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Variable 12 months

    Average litter size: 10

    Recommended wean age: 3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    032242-JAX-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    032242-JAX-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.