Strain Name:
FVB.129S4(B6)-Ptpn1tm1Bbk/Mmjax
Stock Number:
032242-JAX
Citation ID:
RRID:MMRRC_032242-JAX

Gene Information

Ptpn1tm1Bbk
Name: protein tyrosine phosphatase, non-receptor type 1; targeted mutation 1, Barabra B Kahn
Synonyms: PTP-1B-, PTP1B-, Ex1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knockout
Ptpn1
Name: protein tyrosine phosphatase, non-receptor type 1
Synonyms: PTP-1B, PTP1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knockout
NCBI: 19246
HGNC: HGNC:9642
Homologene: 2119
Genetic Alterations
A neomycin cassette was inserted into exon 1.
Genotype Determination
ES Cell Line
J1 derived from 129S4/SvJae
Phenotype
Homozygous: Decreased adiposity and insulin sensitivity; resistant to high fat diet-induced obesity.

Heterozygous: Normal
Mammalian Phenotype Terms
Allelic Composition: Ptpn1tm1Bbk/Ptpn1tm1Bbk (Genetic Background: involves: 129/Sv * C57BL/6 )

Allelic Composition: Ptpn1tm1Bbk/Ptpn1tm1Bbk (Genetic Background: involves: 129S4/SvJae * C57BL/6J )

Allelic Composition: Ptpn1tm1Bbk/Ptpn1+ (Genetic Background: involves: 129S4/SvJae * C57BL/6J )

MeSH Terms
  • Adipose Tissue/physiology
  • Animals
  • Body Weight/genetics
  • Carrier Proteins/genetics
  • Energy Metabolism
  • Female
  • Glucose/metabolism
  • Glucose Tolerance Test
  • Homeostasis
  • Hyperinsulinism/metabolism
  • Insulin Resistance/genetics
  • Ion Channels
  • Leptin/genetics
  • Male
  • Membrane Proteins/genetics
  • Membrane Transport Proteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Mitochondrial Proteins
  • Muscle, Skeletal/metabolism
  • Protein Tyrosine Phosphatase, Non-Receptor Type 1
  • Protein Tyrosine Phosphatases/deficiency
  • Protein Tyrosine Phosphatases/genetics
  • Proteins/genetics
  • RNA, Messenger
  • Uncoupling Protein 1
  • Uncoupling Protein 2
  • Uncoupling Protein 3
Strain Development
The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J. The rusulting hybrids was then backcrossed to FVB/N for 20 generations.
Suggested Control Mice
Wild-type littermates or FVB/N mice
  • Diabetes/Obesity
  • Metabolism
Donor
Benjamin G. Neel, M.D., Ph.D., Ontario Cancer Institute.
Primary Reference
Klaman LD, Boss O, Peroni OD, Kim JK, Martino JL, Zabolotny JM, Moghal N, Lubkin M, Kim YB, Sharpe AH, Stricker-Krongrad A, Shulman GI, Neel BG, Kahn BB. Increased energy expenditure, decreased adiposity, and tissue-specific insulin sensitivity in protein-tyrosine phosphatase 1B-deficient mice. Mol Cell Biol. 2000 Aug;20(15):5479-89. (Medline PMID: 10891488)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
White (albino)
MMRRC Breeding System
Backcross or Sib-mating
While maintaining a live colony, these mice are bred as homozygotes.
Generation
N20+
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Variable 12 months
Average litter size
10
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032242-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032242-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.