Strain Name:
B6.129P2(129S4)-Hprttm12(Ple177-EGFP/cre)Ems/Mmjax
Stock Number:
032924-JAX
Citation ID:
RRID:MMRRC_032924-JAX
Major Collection:

Gene Information

Hprttm12(Ple177-EGFP/cre)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 12, Elizabeth M Simpson
Synonyms: RGS16-C-EGFP/cre, Hprt1tm12(Ple177-EGFPcre;mEMS762)Ems, Hprt1tm12(mEMS762)Ems
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Targeted Mutation
Hprt
Name: hypoxanthine guanine phosphoribosyl transferase
Synonyms: Hprt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Targeted Mutation
NCBI: 15452
HGNC: HGNC:5157
Homologene: 56590
RGS16
Name: regulator of G-protein signaling 16
Type: Gene
Species: Homo sapiens (human)
Chromosome: 1
Alteration at locus: Targeted Mutation
Genetic Alterations
The Ple177-EGFPCre transgene (pEMS1089) was designed with the 1302 bp Ple177 MiniPromoter (derived from an upstream candidate regulatory regions and a subsection of the human regulator of G-protein signaling 16 (RGS16) promoter) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome via electroporation into mEMS21TG2A embryonic stem cells (derived from 129P2/OlaHsd-derived E14TG2a ES cells; a karyotypically male ES cell line harboring the Hprtb-m3 mutation on the X chromosome).
ES Cell Line
E14TG2a derived from 129P2/OlaHsd
Phenotype

Homozygous: Not evaluated

Heterozygous: These Ple177-EGFPCre;mEMS762 mice have the Ple177-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene directing expression of an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFPCre) to hippocampus, cortex, and cerebellum. These Ple177-EGFPCre;mEMS762 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain.

The donating investigator reports: When these Ple177-EGFPCre;mEMS762 mice are bred to also harbor the Gt(ROSA)26Sortm1Sor mutant allele, strong Cre recombinase activity (βGal staining) is detected widely throughout the brain and eye, with notable expression in the hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum as well as in the retina. In each case, the staining appears mosaic, thus not all cells are βGal-positive, but a significant subpopulation of cells across many regions are labeled. This mosaicism is emphasized in the labeling of radial columns of cells in the cortex and the retina. A majority of the βGal-positive cells of the grey matter appear to be neuronal, as they co-label with the neuronal marker NeuN. However, it is apparent that the reporter is not restricted to neurons, but is also detected in cells within the white matter (notably in the corpus callosum, hippocampal fornix and the cerebellar white matter), suggestive of glial labeling as well. EGFP expression was not detected in any region of adult brains, indicating the βGal expression is an historical marker of expression of the EGFP/cre reporter protein. The pattern of Cre recombinase activity (βGal expression) is very similar to that seen for the Ple176 and the Ple178 strains.

MeSH Terms
  • Animals
  • Brain/metabolism
  • Cell Differentiation/genetics
  • Computational Biology
  • Databases, Genetic
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Gene Expression
  • Gene Expression Profiling/statistics & numerical data
  • Gene Knock-In Techniques
  • Genes, Reporter
  • Genomics
  • Humans
  • Mice
  • Mice, Transgenic
  • Neurons/cytology
  • Neurons/metabolism
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid
Strain Development
Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric mice (founder line mEMS762) were bred to B6.129S4-Gt(ROSA)26Sortm1Sor/J mice to establish the mutant colony. These Ple177-EGFPCre;mEMS762 mutant mice were bred with B6.129S4-Gt(ROSA)26Sortm1Sor/J mice for multiple generations. After this, hemizygous sperm was sent to the MMRRC. Upon arrival, Ple177-EGFPCre;mEMS762 mutant mice were backcrossed to C57BL/6J inbred mice (selecting away from the Gt(ROSA)26Sortm1Sor mutation) to establish this congenic strain.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
  • Research Tools
Donor
Elizabeth Simpson, University of British Columbia
Primary Reference
Portales-Casamar E; Swanson DJ; Liu L; de Leeuw CN; Banks KG; Ho Sui SJ; Fulton DL; Ali J; Amirabbasi M; Arenillas DJ; Babyak N; Black SF;onaguro RJ; Brauer E; Candido TR; Castellarin M; Chen J; Chen Y; Cheng JC; Chopra V; Docking TR; Dreolini L; D'Souza CA; Flynn EK; Glenn R; Hatakka K; Hearty TG; Imanian B; Jiang S; Khorasan-zadeh S; Komljenovic I; Laprise S; Liao NY; Lim JS; Lithwick S; Liu F; Liu J; Lu M; McConechy M; McLeod AJ; Milisavljevic M; Mis J; O'Connor K; Palma B; Palmquist DL; Schmouth JF; Swanson MI; Tam B; Ticoll A; Turner JL; Varhol R; Vermeulen J; Watkins RF; Wilson G; Wong BK; Wong SH; Wong TY; Yang GS; Ypsilanti AR; Jones SJ; Holt RA; Goldowitz D; Wasserman WW; Simpson EM. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A 2010 Sep 21;107(38):16589-94 (Medline PMID: 20807748)

Colony and Husbandry Information

The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032924-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032924-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.