Strain Name:
B6NCrl.129-Akap5tm2Gsm/Mmucd
Stock Number:
034250-UCD
Citation ID:
RRID:MMRRC_034250-UCD
Other Names:
AKAP5, B6NCrl.129-Akap5tm2Gsm/Mmcd

Strain Information

Akap5tm2Gsm
Name: A kinase anchor protein 5; targeted mutation 2, G Stanley Mcknight
Synonyms: AKAP150 -
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Akap5
Name: A kinase anchor protein 5
Synonyms: LOC238276, AKAP150, 3526401B18Rik
Type: Gene
Species: Mouse
Chromosome: 12
Alteration at locus: Knockout
NCBI: 238276
HGNC: HGNC:375
Homologene: 15854
Genetic Alterations
Most of the coding sequence for the AKAP5 gene was replaced with a Neo cassette using traditional gene targeting techniques.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: AKAP5 mice appear viable, healthy and fertile. They do present electrophysiological deficits in the hippocampus (LTD and LTP) and show a dramatic delocalization of PKA regulatoy and catalytic subunits in hippocampus and striatum. Mutations in AKAP5 disrupt dendritic signaling complexes and lead to electrophysiological and behavioral phenotypes in mice PLoS One. They also show a delocalization of PKA and adenylate cyclase 5/6 in cardiomyocytes and these myocytes do not respond to isoproterenol challenge with increased calcium transients (Nichols et al. Sympathetic Stimulation of Adult Cardiomyocytes Requires Association of AKAP5 With a Subpopulation of L- Type Calcium Channels and a delocalization of Protein Kinase C in vascular Smooth muscle. AKAP150 is required for stuttering persistent Ca2+ sparklets and angiotensin II- induced hypertension.

Heterozygous: No observable phenotype in heterozygotes.
Mammalian Phenotype Terms
Allelic Composition: Akap5tm2Gsm/Akap5tm2Gsm (Genetic Background: involves: 129S1/SvImJ * C57BL/6 )

MeSH Terms
  • A Kinase Anchor Proteins/deficiency
  • A Kinase Anchor Proteins/genetics
  • A Kinase Anchor Proteins/physiology
  • Animals
  • Binding Sites
  • Corpus Striatum
  • Cyclic AMP-Dependent Protein Kinases/metabolism
  • Dendrites/metabolism
  • Dendrites/pathology
  • Electrophysiological Phenomena/genetics
  • Hippocampus
  • Learning Disorders/etiology
  • Learning Disorders/genetics
  • Memory Disorders/etiology
  • Memory Disorders/genetics
  • Mice
  • Mice, Knockout
  • Microtubule-Associated Proteins/metabolism
  • Neuronal Plasticity
  • Protein Binding
  • Signal Transduction
  • A Kinase Anchor Proteins/metabolism
  • Age Factors
  • Calcium Channels, L-Type/metabolism
  • Calcium Signaling/drug effects
  • Calcium Signaling/physiology
  • Cells, Cultured
  • Cyclic AMP/physiology
  • Cyclic AMP-Dependent Protein Kinases/physiology
  • Isoproterenol/pharmacology
  • Mice, Inbred C57BL
  • Myocytes, Cardiac/drug effects
  • Myocytes, Cardiac/metabolism
  • Myocytes, Cardiac/physiology
  • Receptors, Adrenergic, beta/physiology
  • Signal Transduction/drug effects
  • Signal Transduction/physiology
  • Sympathetic Nervous System/drug effects
  • Sympathetic Nervous System/metabolism
  • Sympathetic Nervous System/physiology
  • Angiotensin II
  • Calcium Signaling
  • Hypertension/etiology
  • Protein Kinase C-alpha/metabolism
  • Vascular Resistance
Strain Development
After generating male chimeras containing the AKAP5 Knockout allele, chimeras were mated to female C57BL/6Crl to obtain AKAP5 heterozygotes which were then backcrossed for over twenty generations to C57BL/6Crl females.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Hematology
  • Immunology and Inflammation
  • Internal/Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Reproduction
  • Research Tools
  • Sensorineural
  • oral biology
Donor
G. Stanley McKnight, Ph.D., University of Washington
Primary Reference
  • Weisenhaus et al.(2010) Mutations in AKAP5 disrupt dendritic signaling complexes and lead to electrophysiological and behavioral phenotypes in mice PLoS One. 2010 Apr 23;5(4) (Medline PMID: 20428246)
  • Nichols et al.(2010)Sympathetic Stimulation of Adult Cardiomyocytes Requires Association of AKAP5 With a Subpopulation of L-Type Calcium Channels. Circ Res. 2010 Jul 29. (Medline PMID: 20671242)
  • Navedo et al.(2009)AKAP150 is required for stuttering persistent Ca2+ sparklets and angiotensin II-induced hypertension.Circ Res. 2008 Feb 1;102(2) (Medline PMID: 18174462)

Colony and Husbandry Information

Mice can be maintained as Heterozygotes or homozygotes and AKAP5 heterozygotes are backcrossed on a regular basis to C57BL/6Crl.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034250-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
034250-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
034250-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.