Name: targeted mutation 1.1, J Larry Jameson
Alteration at locus: Knock-In
Name: estrogen receptor 1 (alpha)
Alteration at locus: Knock-In
ES Cell Line: HM-1
Homozygous phenotype: N/A -heterozygous females are
Heterozygous phenotype: Heterozygous females display inhibited differentiation of mammary gland, decreased serum progesterone, enlarged uteri with cystic endometrial hyperplasia, increased luminal uterine epithelium height, and are anovulatory and infertile.There are consequences for both sexes in bone devlopment, neuroendocrine reproductive axis, and in reproductive behaviour due to the role that estrogen plays in these systems. Heterozygous males are fertile.
Strain of Origin: 129X1/SvJ
Strain genetic background: 129X1/SvJ
Strain Development: Male chimeras were bred to WT 129/SvJ females to achieve germline transmission. Resulting progeny contained the neomycin cassette flanked by loxP sites. Heterozygous males were then bred to transgenic 129S1/SvImJ females expressing cre recombinase under the control of cytomegalovirus promoter. After the neomycin cassette was excised the CMV-cre transgene was bred out of the sucessive generations by selecting for animals with the mutation and without cre. The females with tht mutation are infertile, so all breeding was done through het males and mated to 129S1/SvImJ females. In 2005, we began backcrossing males from the 129S1/SvImJ strain onto a C57B/L6 backround. We maintain this mutation congenic on 129/SvImJ and on C57B/L6 in two separate lines.
Suggested Control Mice:
Donor: J. Larry Jameson, MD. PhD, Northwestern University
Females with mutation are infertile, although they can be superoved to generate ooctyes for IVF or for natural mating with collection of 2-cell or blastocysts. The hormonal feedback does not appear to be sufficient to sustain a pregnancy in mutant females (het).
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.