Strain Name    : C57BL/6J-Adam17m1Btlr/Mmjax

Stock Number : 034295-JAX

Gene Information

(provided by MGI)
Allele Symbol: Adam17m1Btlr
Name: mutation 1, Bruce Beutler
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Adam17
Name: a disintegrin and metallopeptidase domain 17
Chromosome: 12
Alteration at locus: Chemically Induced
Genetic Alterations:

Genotype Determination:

ES Cell Line: Not applicable

Additional mutations found on this strain:

AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00555:Adam17 APN 12 21328109 missense probably damaging 1.00
IGL01340:Adam17 APN 12 21330057 nonsense probably null 0.00
IGL01973:Adam17 APN 12 21349943 missense possibly damaging 0.92
IGL02223:Adam17 APN 12 21361705 missense probably benign 0.00
R0014:Adam17 UTSW 12 21336644 missense possibly damaging 0.86
R0080:Adam17 UTSW 12 21329048 splice donor site probably benign
R0082:Adam17 UTSW 12 21329048 splice donor site probably benign
R0324:Adam17 UTSW 12 21349938 missense probably benign 0.00
R0511:Adam17 UTSW 12 21340458 splice donor site probably benign
R0745:Adam17 UTSW 12 21332221 splice acceptor site probably benign
R1314:Adam17 UTSW 12 21329071 missense probably damaging 1.00
R1503:Adam17 UTSW 12 21332560 splice donor site
R1547:Adam17 UTSW 12 21353957 missense probably benign 0.07
R1594:Adam17 UTSW 12 21340470 critical splice donor site probably null
R1607:Adam17 UTSW 12 21334138 missense probably damaging 1.00
R1812:Adam17 UTSW 12 21361767 missense possibly damaging 0.73
R2020:Adam17 UTSW 12 21349875 missense probably damaging 1.00
R2249:Adam17 UTSW 12 21346011 splice donor site
R2679:Adam17 UTSW 12 21355714 splice acceptor site
R3408:Adam17 UTSW 12 21329118 missense possibly damaging 0.82
R3735:Adam17 UTSW 12 21325412 missense probably benign 0.05
R3793:Adam17 UTSW 12 21346005 splice donor site
R3886:Adam17 UTSW 12 21325587 missense probably damaging 1.00
R3888:Adam17 UTSW 12 21325587 missense probably damaging 1.00
R4062:Adam17 UTSW 12 21325457 missense probably damaging 1.00
R4415:Adam17 UTSW 12 21345701 missense not run
X0063:Adam17 UTSW 12 21332585 missense probably benign 0.02

Phenotype

Homozygous phenotype: Mice homozygous for the mutation are viable and are characterized by open eyelids and curly vibrissae at birth; adult animals display wavy coats and corneal opacities. These animals are susceptible to dextran sulfate sodium (DSS)-induced colitis and exhibit severe weight loss as a result of DSS administration. The donating investigator indicates that homozygous males are fertile, however homozygous females do not breed. This mutant mouse strain may be useful in studies of inflammatory bowel disease.

For additional information, see The Scripps Research Institute Mutagenix website.


Heterozygous phenotype: Not evaluated, phenotype is presumed to be normal.

Mammalian Phenotype Terms:
Allelic Composition: Adam17m1Btlr/Adam17m1Btlr (Genetic Background: C57BL/6J-Adam17m1Btlr )

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results in an T to A transversion at position 1186 of the Adam17 gene, in exon 9 of 19 total exons using Genbank record NM_009615. The mutation alters the corresponding amino acid from phenylalanine to isoleucine at codon 343. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Suggested Control Mice: Wild-type littermates

  • Immunology and Inflammation
  • Dermatology
  • Developmental Biology

Donor: Bruce Beutler, Ph.D., The Scripps Research Institute

Primary Reference:

  • Brandl K, Sun L, Neppl C, Siggs OM, Le Gall SM, Tomisato W, Li X, Du X, Maennel DN, Blobel CP, Beutler B, Myd88 signaling in nonhematopoietic cells protects mice against induced colitis by regulating specific EGF receptor ligands, PNAS 2010, Nov 16;107(46):19967-72 (Medline PMID: 21041656)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as heterozygote female x homozygote male. Females homozygous for the mutation do not breed.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
034295-JAX-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
034295-JAX-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.