Strain Detail Sheet

Strain Name    :

C57BL/6J-Adam17m1Btlr/Mmjax

Stock Number :

034295-JAX

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Adam17m1Btlr
Name: mutation 1, Bruce Beutler
Alteration at locus: Chemically Induced
Gene Symbol: Adam17
Name: a disintegrin and metallopeptidase domain 17
Chromosome: 12
Alteration at locus: Chemically Induced

Genetic Alterations:

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Mice homozygous for the mutation are viable and are characterized by open eyelids and curly vibrissae at birth; adult animals display wavy coats and corneal opacities. These animals are susceptible to dextran sulfate sodium (DSS)-induced colitis and exhibit severe weight loss as a result of DSS administration. The donating investigator indicates that homozygous males are fertile, however homozygous females do not breed. This mutant mouse strain may be useful in studies of inflammatory bowel disease.

For additional information, see The Scripps Research Institute Mutagenix website.


Heterozygous phenotype: Not evaluated, phenotype is presumed to be normal.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Adam17m1Btlr/Adam17m1Btlr
        C57BL/6J-Adam17<m1Btlr>
  • mortality/aging
    • decreased survivor rate (MGI Ref ID J:166602)
      • survival rate of homozygotes (16.07%) is slightly lower than the expected Mendelian ratio (25%)
  • immune system phenotype
    • decreased circulating tumor necrosis factor level (MGI Ref ID J:166602)
      • LPS-stimulated peritoneal macrophages exhibit increased surface expression of TNF, but decreased quantities of soluble TNF
      • PMA-stimulated embryonic fibroblasts secrete less TNF than heterozygous controls
    • increased susceptibility to induced colitis (MGI Ref ID J:166602)
  • homeostasis/metabolism phenotype
    • decreased circulating tumor necrosis factor level (MGI Ref ID J:166602)
      • LPS-stimulated peritoneal macrophages exhibit increased surface expression of TNF, but decreased quantities of soluble TNF
      • PMA-stimulated embryonic fibroblasts secrete less TNF than heterozygous controls
    • decreased transforming growth factor level (MGI Ref ID J:166602)
      • PMA-stimulated embryonic fibroblasts secrete less TGFalpha than controls
  • growth/size phenotype
    • weight loss (MGI Ref ID J:166602)
      • significant weight loss is observed with addition of 1% dextran sodium sulfate (DSS) to water
  • digestive/alimentary phenotype
    • increased susceptibility to induced colitis (MGI Ref ID J:166602)
  • integument phenotype
    • waved hair (MGI Ref ID J:166602)
      • wavy coat

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results in an T to A transversion at position 1186 of the Adam17 gene, in exon 9 of 19 total exons using Genbank record NM_009615. The mutation alters the corresponding amino acid from phenylalanine to isoleucine at codon 343. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Immunology and Inflammation
  • Dermatology
  • Developmental Biology

Strain Origin

Donor: Bruce Beutler, Ph.D., The Scripps Research Institute

Primary Reference:

  • Brandl K, Sun L, Neppl C, Siggs OM, Le Gall SM, Tomisato W, Li X, Du X, Maennel DN, Blobel CP, Beutler B, Myd88 signaling in nonhematopoietic cells protects mice against induced colitis by regulating specific EGF receptor ligands, PNAS 2010, Nov 16;107(46):19967-72 (Medline PMID: 21041656)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

Special Considerations

While maintaining a live colony, these mice are bred as heterozygote female x homozygote male. Females homozygous for the mutation do not breed.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034295-JAX-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
034295-JAX-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/20/2014