Strain Detail Sheet

Strain Name    :

129S6-Nrcamtm1Gmt/Mmucd

Stock Number :

000370-UCD

Other Names   :

NrCAM KO, 129S6-Nrcamtm1Gmt/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Nrcamtm1Gmt
Name: targeted mutation 1, Martin Grumet
Alteration at locus: Knockout
Gene Symbol: Nrcam
Name: neuron-glia-CAM-related cell adhesion molecule
Chromosome: 12
Alteration at locus: Knockout

Genetic Alterations:
a neo cassette was inserted into exon 4.

Genotype Determination:

ES Cell Line: W4 derived from 129S6/SvEvTac

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Mild cerebellar defect which is exacerbated in L1 null background. Delayed development of node of Ranvier. Deficits in co-ordination and learning. Old mice develop cataracts.

Heterozygous phenotype: Little or none.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Nrcamtm1Gmt/Nrcamtm1Gmt
        either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Swiss Webster)
  • nervous system phenotype
    • abnormal neurite morphology (MGI Ref ID J:71828)
      • isolated cerebellar cells fail to extend neurites in response to contactin, however neurites still extend in response to RPTPbeta
    • small cerebellum (MGI Ref ID J:71828)
      • reduced size of lobes IV and V; these lobes contained 11% less gray matter than control littermates; lobes I, II and X are also slightly reduced in size
Nrcamtm1Gmt/Nrcamtm1Gmt
        involves: 129S6/SvEvTac
  • nervous system phenotype
    • nervous system phenotype (MGI Ref ID J:136656)
      • Purkinje dendrites appear normal


The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Nrcamtm1Gmt/Nrcamtm1Gmt
        involves: 129S6/SvEvTac * ICR
  • nervous system phenotype
    • abnormal axon morphology (MGI Ref ID J:166099)
      • sodium ion channels are absent from heminodes unlike in wild-type mice
      • heminodes fail to form unlike in wild-type mice
      • however, nodes form normally
Nrcamtm1Gmt/Nrcamtm1Gmt
        involves: 129S6/SvEvTac * Swiss Webster
  • behavior/neurological phenotype
    • abnormal learning/ memory (MGI Ref ID J:153165)
      • males and females display a longer latency to reach the escape platform in the reversal phase
      • abnormal motor learning (MGI Ref ID J:153165)
        • in a rotarod test females, but not males, show a shorter latency to fall in retests suggesting an impairment in the improvement normally seen across trials
      • abnormal spatial learning (MGI Ref ID J:153165)
        • females but not males display a longer latency to find a hidden platform
    • abnormal locomotor activation (MGI Ref ID J:153165)
      • in a 1 h open field test females display decreased activity during the beginning of the test and increased activity towards the end of the test relative to control females
      • decreased vertical activity (MGI Ref ID J:153165)
        • decrease in rearing frequency in an open field in females
    • abnormal social investigation (MGI Ref ID J:153165)
      • males fail to show a preference for a stranger mouse over an empty cage
  • nervous system phenotype
    • decreased prepulse inhibition (MGI Ref ID J:153165)
      • small but significant decrease in males

Strain of Origin: 129X1/SvJ

Strain genetic background: 129S6/SvEvTac

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Research Tools
  • Sensorineural

Strain Origin

Donor: Martin Grumet, Ph.D., Rutgers University

Primary Reference:

  • Sakurai T, Lustig M, Babiarz J, Furley AJ, Tait S, Brophy PJ, Brown SA, Brown LY, Mason CA, Grumet M. Overlapping functions of the cell adhesion molecules Nr-CAM and L1 in cerebellar granule cell development. J Cell Biol. 2001 Sep 17;154(6):1259-73. Erratum in: J Cell Biol 2001 Oct 15;155(2):311. (Medline PMID: 11564762)
  • Custer AW, Kazarinova-Noyes K, Sakurai T, Xu X, Simon W, Grumet M, Shrager P. The role of the ankyrin-binding protein NrCAM in node of Ranvier formation. J Neurosci. 2003 Nov 5;23(31):10032-9. (Medline PMID: 14602817)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000370-UCD-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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