Strain Name:
STOCK Tsg101tm1Kuw/Mmmh
Stock Number:
037407-MU
Citation ID:
RRID:MMRRC_037407-MU
Other Names:
Tsg101 floxed

Gene Information

Tsg101tm1Kuw
Name: tumor susceptibility gene 101; targeted mutation 1, Kay-Uwe Wagner
Synonyms: Tsg101fl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Conditional
Tsg101
Name: tumor susceptibility gene 101
Synonyms: CC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Conditional
NCBI: 22088
Homologene: 4584
Genetic Alterations
Exon 1 was flanked by a floxed neo cassette inserted approximately 3 kb upstream and a single loxP site inserted 230 bp into intron 1. 
Genotype Determination
ES Cell Line
RW-4 derived from 129X1/SvJ
Phenotype

Homozygous: No observable phenotype.

Heterozygous: No observable phenotype.

Expected Phenotype After Cre-Excision: Cell death, cell cycle arrest.

Mammalian Phenotype Terms
Allelic Composition: Tsg101tm1Kuw/Tsg101tm1Kuw (Genetic Background: Not Specified )

MeSH Terms
  • Animals
  • Cell Differentiation/genetics
  • Cell Division/genetics
  • Cell Survival/genetics
  • DNA-Binding Proteins/physiology
  • Embryo, Mammalian/cytology
  • Embryo, Mammalian/physiology
  • Endosomal Sorting Complexes Required for Transport
  • Epithelial Cells/physiology
  • Female
  • Fetal Death/genetics
  • Gene Expression Regulation, Developmental
  • Lactation Disorders/genetics
  • Mammary Glands, Animal/abnormalities
  • Mammary Glands, Animal/cytology
  • Mammary Glands, Animal/physiology
  • Mammary Neoplasms, Experimental/genetics
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Transcription Factors/physiology
  • Cell Cycle/physiology
  • Cell Death/physiology
  • Cell Division
  • Cell Survival
  • Cloning, Molecular
  • DNA-Binding Proteins/genetics
  • Embryo, Mammalian
  • Fibroblasts/cytology
  • G1 Phase/physiology
  • Leucine Zippers
  • Mutagenesis
  • Recombinant Proteins/metabolism
  • S Phase/physiology
  • Sequence Deletion
  • Transcription Factors/genetics
  • Tumor Suppressor Protein p53/metabolism
Strain Development
After targeted insertion of loxP sites and electroporation of correctly targeted RW-4 ES cells derived from 129X1/SvJ, chimeras were generated. The chimeras were bred with C57BL/6 and the line was maintained by sib-mating for 30 generations. Then offspring were backcrossed onto FVB for 3 generations to increase the number of pups and for use in mammary cancer studies.
Suggested Control Mice
Wild-type littermates
  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Internal Organ
  • Models for Human Disease
    • Mammary cancers
Donor
Kay-Uwe Wagner, Ph.D., University of Nebraska Medical Center.
Primary Reference
  • Wagner KU, Krempler A, Qi Y, Park K, Henry MD, Triplett AA, Riedlinger G, Rucker III EB, Hennighausen L. Tsg101 is essential for cell growth, proliferation, and cell survival of embryonic and adult tissues. Mol Cell Biol. 2003 Jan;23(1):150-62. (Medline PMID: 12482969)
  • Krempler A, Henry MD, Triplett AA, Wagner KU. Targeted deletion of the Tsg101 gene results in cell cycle arrest at G1/S and p53-independent cell death. J Biol Chem. 2002 Nov 8;277(45):43216-23. (Medline PMID: 12205095)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
  • Agouti
  • Grey
  • White
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Backcross:
    • Wild-type (FVB) female x Heterozygous male or reciprocal mating
  • Sib-mating:
    • Wild-type female x Heterozygous male littermate or reciprocal mating
    • Heterozygous female x Heterozygous male littermate
Generation
N3
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
037407-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037407-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.