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Strain Name:
C57BL/6J-MtgxR0025Btlr/Mmmh
Stock Number:
038320-MU
Citation ID:
RRID:MMRRC_038320-MU
Other Names:
R0025 (G1), C57BL/6J-MtgxR0025Btlr
Major Collection:

Strain Information

Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Pmp22
Name: peripheral myelin protein 22
Synonyms: Gas-3, TRE002
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18858
HGNC: HGNC:9118
Homologene: 7482
Mov10
Name: Mov10 RISC complex RNA helicase
Synonyms: Mov-10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17454
HGNC: HGNC:7200
Homologene: 10365
Mbd4
Name: methyl-CpG binding domain protein 4
Synonyms: Med1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17193
HGNC: HGNC:6919
Homologene: 2916
Wdr47
Name: WD repeat domain 47
Synonyms: 1810073M12Rik, nemitin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99512
Homologene: 8984
Rps6kb1
Name: ribosomal protein S6 kinase, polypeptide 1
Synonyms: p70/85s6k, p70s6k, S6K1, 2610318I15Rik, p70S6K1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72508
Homologene: 81703
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 93,042,358 bp
  • T to C, chromosome 1 at 174,791,314 bp
  • T to C, chromosome 1 at 181,913,373 bp
  • T to C, chromosome 2 at 26,470,931 bp
  • A to G, chromosome 2 at 34,986,292 bp
  • A to T, chromosome 2 at 52,222,774 bp
  • T to C, chromosome 2 at 57,108,615 bp
  • C to A, chromosome 2 at 98,666,580 bp
  • T to A, chromosome 2 at 112,009,020 bp
  • G to T, chromosome 2 at 112,459,485 bp
  • T to C, chromosome 2 at 136,100,784 bp
  • T to C, chromosome 2 at 145,878,832 bp
  • T to C, chromosome 3 at 45,380,499 bp
  • A to T, chromosome 3 at 69,748,321 bp
  • G to A, chromosome 3 at 95,140,028 bp
  • A to G, chromosome 3 at 95,550,137 bp
  • T to C, chromosome 3 at 95,585,125 bp
  • A to C, chromosome 3 at 104,804,603 bp
  • A to G, chromosome 3 at 107,748,644 bp
  • G to T, chromosome 3 at 108,637,991 bp
  • C to T, chromosome 3 at 116,534,783 bp
  • A to G, chromosome 4 at 63,275,977 bp
  • C to A, chromosome 4 at 88,408,959 bp
  • T to C, chromosome 4 at 106,748,174 bp
  • G to T, chromosome 4 at 117,165,517 bp
  • A to T, chromosome 4 at 124,921,254 bp
  • G to T, chromosome 4 at 154,160,345 bp
  • C to T, chromosome 4 at 155,426,115 bp
  • T to C, chromosome 5 at 6,771,665 bp
  • T to C, chromosome 5 at 34,643,953 bp
  • T to A, chromosome 5 at 38,092,564 bp
  • T to C, chromosome 5 at 90,250,405 bp
  • T to C, chromosome 5 at 101,845,046 bp
  • A to G, chromosome 5 at 123,927,235 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • C to T, chromosome 6 at 58,874,308 bp
  • A to G, chromosome 6 at 115,844,568 bp
  • C to T, chromosome 6 at 123,331,547 bp
  • T to A, chromosome 6 at 125,682,812 bp
  • T to C, chromosome 7 at 15,987,511 bp
  • A to T, chromosome 7 at 26,200,813 bp
  • G to A, chromosome 7 at 28,564,283 bp
  • A to T, chromosome 7 at 43,351,925 bp
  • A to G, chromosome 7 at 98,467,752 bp
  • A to G, chromosome 7 at 106,823,756 bp
  • A to T, chromosome 7 at 107,181,258 bp
  • G to A, chromosome 7 at 110,793,669 bp
  • G to A, chromosome 7 at 118,212,443 bp
  • A to T, chromosome 7 at 119,658,315 bp
  • A to G, chromosome 7 at 130,503,793 bp
  • T to C, chromosome 7 at 140,813,272 bp
  • A to G, chromosome 8 at 43,185,432 bp
  • A to G, chromosome 8 at 47,066,000 bp
  • T to G, chromosome 8 at 72,381,497 bp
  • T to C, chromosome 8 at 93,271,885 bp
  • A to G, chromosome 8 at 104,965,996 bp
  • T to G, chromosome 8 at 112,803,164 bp
  • C to A, chromosome 8 at 127,161,308 bp
  • A to G, chromosome 9 at 40,092,253 bp
  • A to G, chromosome 9 at 57,682,061 bp
  • G to T, chromosome 9 at 106,913,268 bp
  • T to A, chromosome 9 at 110,840,894 bp
  • C to A, chromosome 9 at 119,670,484 bp
  • A to G, chromosome 9 at 123,157,025 bp
  • A to G, chromosome 9 at 123,829,009 bp
  • A to T, chromosome 10 at 9,762,748 bp
  • A to G, chromosome 10 at 59,788,877 bp
  • A to T, chromosome 10 at 100,537,831 bp
  • A to C, chromosome 11 at 16,985,594 bp
  • A to T, chromosome 11 at 31,365,559 bp
  • T to G, chromosome 11 at 53,979,688 bp
  • A to G, chromosome 11 at 55,162,795 bp
  • T to C, chromosome 11 at 60,230,134 bp
  • A to T, chromosome 11 at 63,158,250 bp
  • G to A, chromosome 11 at 65,969,955 bp
  • T to C, chromosome 11 at 68,836,173 bp
  • A to G, chromosome 11 at 69,394,966 bp
  • C to A, chromosome 11 at 70,526,345 bp
  • T to C, chromosome 11 at 70,613,042 bp
  • AGAGGAGGAGGAGGAGG to AGAGGAGGAGGAGG, chromosome 11 at 76,000,115 bp
  • A to T, chromosome 11 at 86,511,587 bp
  • C to T, chromosome 11 at 106,324,560 bp
  • T to C, chromosome 12 at 30,100,390 bp
  • T to A, chromosome 12 at 112,785,534 bp
  • A to G, chromosome 13 at 46,786,511 bp
  • G to A, chromosome 13 at 59,500,200 bp
  • A to T, chromosome 13 at 59,805,328 bp
  • T to A, chromosome 13 at 67,257,898 bp
  • A to T, chromosome 13 at 100,779,781 bp
  • T to A, chromosome 13 at 104,036,891 bp
  • A to T, chromosome 13 at 106,546,867 bp
  • A to G, chromosome 13 at 111,756,129 bp
  • G to A, chromosome 13 at 114,870,496 bp
  • T to C, chromosome 15 at 89,186,183 bp
  • C to T, chromosome 15 at 98,142,671 bp
  • A to G, chromosome 16 at 64,784,818 bp
  • A to G, chromosome 17 at 24,631,004 bp
  • A to G, chromosome 17 at 31,847,275 bp
  • A to T, chromosome 17 at 34,514,299 bp
  • A to G, chromosome 17 at 36,641,755 bp
  • G to T, chromosome 17 at 37,067,210 bp
  • A to C, chromosome 17 at 46,919,771 bp
  • G to A, chromosome 17 at 78,599,835 bp
  • T to A, chromosome 18 at 32,859,307 bp
  • G to T, chromosome 18 at 36,531,666 bp
  • T to C, chromosome 18 at 48,047,739 bp
  • T to A, chromosome 18 at 60,390,787 bp
  • T to G, chromosome 19 at 5,707,014 bp
  • T to C, chromosome 19 at 7,285,922 bp
  • T to C, chromosome 19 at 11,983,958 bp
  • A to T, chromosome 19 at 42,266,839 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0025 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038320-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.