Strain Name:
C57BL/6J-MtgxR0089Btlr/Mmmh
Stock Number:
038376-MU
Citation ID:
RRID:MMRRC_038376-MU
Other Names:
R0089 (G1), C57BL/6J-MtgxR0089Btlr
Major Collection:

Gene Information

Gene Symbol: Slc26a5 [MGI:1933154] (Mus musculus (mouse))
Name: solute carrier family 26, member 5
Synonyms: Pres, prestin
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Sez6 [MGI:104745] (Mus musculus (mouse))
Name: seizure related gene 6
Synonyms: D11Bhm177e, sez-6
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Zfand5 [MGI:1278334] (Mus musculus (mouse))
Name: zinc finger, AN1-type domain 5
Synonyms: Za20d2, 2310057A04Rik, 5830475F03Rik, Zfp216
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Trim13 [MGI:1913847] (Mus musculus (mouse))
Name: tripartite motif-containing 13
Synonyms: RNF77, Rfp2, 3110001L12Rik, LEU5
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Gmps [MGI:2448526] (Mus musculus (mouse))
Name: guanine monophosphate synthetase
Synonyms: Gm9479
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Birc6 [MGI:1276108] (Mus musculus (mouse))
Name: baculoviral IAP repeat-containing 6
Synonyms: apollon, D630005A10Rik, A430032G04Rik, A430040A19Rik, Bruce
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Fry [MGI:2443895] (Mus musculus (mouse))
Name: FRY microtubule binding protein
Synonyms: cg003, 9330186A19Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 6,848,948 bp
  • T to C, chromosome 1 at 166,667,309 bp
  • C to T, chromosome 1 at 178,046,989 bp
  • G to A, chromosome 2 at 36,453,095 bp
  • C to A, chromosome 2 at 76,729,200 bp
  • A to C, chromosome 2 at 86,159,574 bp
  • T to C, chromosome 2 at 88,005,987 bp
  • A to G, chromosome 2 at 157,199,414 bp
  • T to C, chromosome 3 at 63,998,698 bp
  • A to G, chromosome 3 at 122,275,462 bp
  • T to G, chromosome 4 at 3,748,768 bp
  • A to G, chromosome 4 at 116,035,773 bp
  • T to C, chromosome 4 at 134,081,194 bp
  • T to C, chromosome 4 at 142,031,925 bp
  • T to A, chromosome 4 at 144,555,733 bp
  • A to C, chromosome 5 at 21,811,344 bp
  • C to T, chromosome 5 at 35,094,888 bp
  • T to C, chromosome 5 at 38,155,630 bp
  • T to A, chromosome 5 at 87,420,558 bp
  • T to A, chromosome 5 at 118,008,143 bp
  • A to T, chromosome 5 at 150,340,427 bp
  • T to C, chromosome 5 at 151,584,804 bp
  • A to G, chromosome 6 at 3,487,094 bp
  • A to C, chromosome 6 at 13,220,005 bp
  • G to T, chromosome 6 at 29,206,326 bp
  • T to A, chromosome 6 at 57,900,528 bp
  • C to T, chromosome 6 at 146,350,022 bp
  • A to G, chromosome 7 at 5,056,221 bp
  • G to A, chromosome 7 at 30,318,919 bp
  • T to A, chromosome 7 at 66,712,048 bp
  • G to T, chromosome 7 at 89,893,116 bp
  • T to A, chromosome 7 at 105,057,090 bp
  • T to C, chromosome 7 at 110,050,765 bp
  • T to C, chromosome 7 at 140,652,311 bp
  • T to C, chromosome 8 at 64,982,928 bp
  • A to T, chromosome 8 at 80,615,878 bp
  • C to T, chromosome 8 at 84,176,134 bp
  • A to T, chromosome 8 at 89,030,268 bp
  • T to C, chromosome 8 at 105,846,368 bp
  • T to C, chromosome 9 at 15,938,205 bp
  • T to A, chromosome 9 at 21,591,966 bp
  • A to G, chromosome 9 at 24,695,793 bp
  • C to T, chromosome 9 at 31,316,230 bp
  • A to G, chromosome 9 at 66,798,330 bp
  • G to A, chromosome 9 at 78,473,872 bp
  • T to C, chromosome 9 at 110,372,222 bp
  • A to T, chromosome 9 at 111,392,910 bp
  • G to T, chromosome 10 at 17,908,100 bp
  • C to T, chromosome 10 at 130,386,719 bp
  • T to A, chromosome 11 at 9,292,886 bp
  • T to C, chromosome 11 at 11,934,192 bp
  • G to A, chromosome 11 at 49,524,206 bp
  • G to A, chromosome 11 at 50,859,965 bp
  • A to G, chromosome 11 at 59,000,062 bp
  • T to C, chromosome 11 at 69,104,043 bp
  • T to C, chromosome 11 at 77,974,344 bp
  • A to T, chromosome 11 at 99,075,198 bp
  • T to C, chromosome 11 at 103,103,993 bp
  • G to A, chromosome 11 at 106,009,022 bp
  • T to C, chromosome 12 at 75,963,876 bp
  • T to C, chromosome 12 at 84,016,934 bp
  • A to T, chromosome 12 at 87,773,513 bp
  • T to C, chromosome 12 at 112,177,403 bp
  • A to T, chromosome 13 at 6,555,639 bp
  • T to C, chromosome 14 at 50,392,864 bp
  • A to G, chromosome 14 at 52,149,384 bp
  • G to A, chromosome 14 at 56,514,106 bp
  • C to T, chromosome 14 at 61,604,717 bp
  • A to G, chromosome 14 at 63,528,247 bp
  • T to C, chromosome 15 at 88,701,198 bp
  • C to T, chromosome 16 at 33,763,615 bp
  • A to T, chromosome 17 at 25,885,148 bp
  • T to A, chromosome 17 at 74,638,376 bp
  • A to G, chromosome 18 at 7,439,555 bp
  • T to A, chromosome 18 at 30,303,078 bp
  • A to G, chromosome 18 at 36,640,356 bp
  • A to T, chromosome 19 at 8,370,197 bp
  • C to A, chromosome 19 at 21,279,758 bp
  • G to T, chromosome 19 at 57,043,031 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R0089 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    038376-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.