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Strain Name:
C57BL/6J-MtgxR0200Btlr/Mmmh
Stock Number:
038457-MU
Citation ID:
RRID:MMRRC_038457-MU
Other Names:
R0200 (G1), C57BL/6J-MtgxR0200Btlr
Major Collection:

Strain Information

Hps5
Name: HPS5, biogenesis of lysosomal organelles complex 2 subunit 2
Synonyms: ru-2, ruby eye 2, ru2, Hermansky-Pudlak syndrome 5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 246694
Homologene: 35333
Aatf
Name: apoptosis antagonizing transcription factor
Synonyms: 4933415H02Rik, Trb, 5830465M17Rik, Che-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56321
Homologene: 40811
Lyz2
Name: lysozyme 2
Synonyms: Lzp, Lzm, Lys, Lzm-s1, Lyzs, Lysm
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17105
VEGA: 10
HGNC: HGNC:6740
Homologene: 121490
Dhx35
Name: DEAH-box helicase 35
Synonyms: Ddx35, 1200009D07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71715
Homologene: 6406
Slc7a7
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 7
Synonyms: my+lat1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 20540
Homologene: 88701
Plxdc1
Name: plexin domain containing 1
Synonyms: Tem7, 2410003I07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72324
Homologene: 10700
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 3,670,663 bp
  • T to A, chromosome 1 at 40,441,303 bp
  • T to A, chromosome 1 at 44,013,654 bp
  • A to T, chromosome 1 at 80,281,561 bp
  • C to T, chromosome 1 at 97,894,401 bp
  • T to A, chromosome 1 at 106,004,123 bp
  • T to C, chromosome 1 at 174,117,512 bp
  • A to C, chromosome 2 at 105,297,917 bp
  • A to G, chromosome 2 at 109,970,690 bp
  • A to G, chromosome 2 at 112,480,720 bp
  • T to A, chromosome 2 at 122,527,447 bp
  • T to A, chromosome 2 at 130,152,945 bp
  • C to A, chromosome 2 at 152,622,412 bp
  • A to T, chromosome 2 at 158,829,623 bp
  • T to G, chromosome 2 at 166,795,683 bp
  • C to A, chromosome 2 at 168,223,155 bp
  • C to T, chromosome 2 at 170,115,462 bp
  • T to C, chromosome 2 at 181,000,438 bp
  • A to T, chromosome 3 at 67,180,861 bp
  • T to A, chromosome 3 at 107,105,160 bp
  • A to G, chromosome 3 at 115,928,703 bp
  • A to G, chromosome 3 at 129,706,233 bp
  • A to G, chromosome 3 at 129,737,549 bp
  • T to C, chromosome 4 at 14,621,317 bp
  • A to G, chromosome 4 at 120,483,831 bp
  • T to A, chromosome 4 at 147,862,263 bp
  • A to G, chromosome 4 at 149,898,216 bp
  • T to A, chromosome 5 at 43,261,776 bp
  • T to C, chromosome 5 at 75,163,777 bp
  • T to C, chromosome 5 at 88,493,027 bp
  • G to A, chromosome 5 at 101,814,433 bp
  • T to A, chromosome 5 at 104,977,610 bp
  • T to C, chromosome 5 at 124,384,541 bp
  • A to G, chromosome 5 at 131,487,688 bp
  • C to T, chromosome 6 at 32,197,088 bp
  • T to G, chromosome 6 at 48,486,415 bp
  • T to C, chromosome 6 at 55,897,956 bp
  • T to C, chromosome 6 at 57,432,099 bp
  • T to A, chromosome 6 at 73,069,420 bp
  • T to C, chromosome 6 at 83,967,354 bp
  • T to C, chromosome 6 at 89,323,593 bp
  • T to G, chromosome 6 at 120,761,797 bp
  • T to A, chromosome 6 at 131,168,123 bp
  • C to T, chromosome 6 at 139,990,733 bp
  • T to A, chromosome 7 at 5,544,380 bp
  • A to G, chromosome 7 at 5,883,818 bp
  • T to C, chromosome 7 at 24,432,006 bp
  • T to A, chromosome 7 at 24,640,231 bp
  • T to A, chromosome 7 at 43,885,361 bp
  • T to C, chromosome 7 at 46,778,011 bp
  • T to A, chromosome 7 at 122,671,785 bp
  • C to T, chromosome 7 at 132,610,677 bp
  • T to C, chromosome 7 at 133,974,416 bp
  • T to C, chromosome 7 at 140,400,875 bp
  • T to G, chromosome 8 at 23,096,812 bp
  • T to C, chromosome 8 at 105,951,617 bp
  • A to G, chromosome 8 at 121,784,833 bp
  • C to A, chromosome 9 at 76,951,935 bp
  • C to T, chromosome 9 at 110,561,262 bp
  • A to G, chromosome 10 at 40,040,616 bp
  • A to G, chromosome 10 at 54,074,498 bp
  • T to C, chromosome 10 at 76,607,714 bp
  • C to T, chromosome 10 at 98,979,814 bp
  • T to C, chromosome 10 at 107,685,157 bp
  • T to A, chromosome 10 at 117,280,773 bp
  • C to A, chromosome 10 at 128,634,676 bp
  • T to A, chromosome 11 at 49,135,047 bp
  • C to A, chromosome 11 at 75,672,182 bp
  • T to C, chromosome 11 at 78,361,040 bp
  • T to C, chromosome 11 at 84,445,676 bp
  • T to C, chromosome 11 at 89,441,966 bp
  • T to C, chromosome 11 at 94,355,074 bp
  • T to C, chromosome 11 at 97,934,012 bp
  • T to C, chromosome 11 at 99,985,297 bp
  • A to G, chromosome 11 at 120,413,681 bp
  • T to A, chromosome 12 at 98,663,169 bp
  • A to T, chromosome 13 at 49,726,202 bp
  • T to A, chromosome 13 at 96,496,822 bp
  • A to T, chromosome 14 at 20,448,563 bp
  • A to G, chromosome 14 at 30,102,339 bp
  • A to C, chromosome 14 at 51,996,974 bp
  • A to G, chromosome 14 at 54,377,802 bp
  • A to G, chromosome 14 at 78,510,753 bp
  • C to A, chromosome 15 at 89,826,941 bp
  • G to A, chromosome 16 at 15,629,639 bp
  • A to G, chromosome 16 at 29,614,129 bp
  • T to C, chromosome 16 at 36,323,024 bp
  • A to G, chromosome 16 at 56,123,873 bp
  • C to T, chromosome 17 at 27,145,025 bp
  • T to G, chromosome 17 at 29,526,310 bp
  • A to T, chromosome 17 at 37,012,419 bp
  • A to T, chromosome 17 at 80,251,473 bp
  • T to C, chromosome 18 at 12,219,204 bp
  • T to C, chromosome 18 at 12,524,493 bp
  • A to T, chromosome 18 at 20,340,938 bp
  • A to G, chromosome 18 at 59,062,459 bp
  • T to C, chromosome 19 at 53,635,836 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0200 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038457-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.