Strain Name:
C57BL/6J-MtgxR0225Btlr/Mmmh
Stock Number:
038470-MU
Citation ID:
RRID:MMRRC_038470-MU
Other Names:
R0225 (G1), C57BL/6J-MtgxR0225Btlr
Major Collection:

Gene Information

Kat2b
Name: K(lysine) acetyltransferase 2B
Synonyms: A930006P13Rik, Pcaf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 18519
HGNC: HGNC:8638
Homologene: 20834
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Lhx9
Name: LIM homeobox protein 9
Synonyms: LH2B, 3110009O07Rik, Lhx9 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16876
Homologene: 7816
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: Pdzgef1, nRapGEP, RA-GEF-1, 5830453M24Rik, CNRasGEF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 76089
Homologene: 35477
Lgi3
Name: leucine-rich repeat LGI family, member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 213469
VEGA: 14
Homologene: 16406
Hira
Name: histone cell cycle regulator
Synonyms: Tuple1, Gm15797, D16Ertd95e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 15260
HGNC: HGNC:4916
Homologene: 48172
Phf3
Name: PHD finger protein 3
Synonyms: 2310061N19Rik, AU020177
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 213109
HGNC: HGNC:8921
Homologene: 9040
Esyt2
Name: extended synaptotagmin-like protein 2
Synonyms: Fam62b, D12Ertd551e, 2410017M09Rik, 4921504I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 52635
VEGA: 12
Homologene: 32699
Bicd1
Name: BICD cargo adaptor 1
Synonyms: B830009D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 12121
HGNC: HGNC:1049
Homologene: 37518
Il3
Name: interleukin 3
Synonyms: BPA, HCGF, MCGF, PSF, Csfmu, Il-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 16187
HGNC: HGNC:6011
Homologene: 49243
Hdac2
Name: histone deacetylase 2
Synonyms: Yy1bp, D10Wsu179e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 15182
HGNC: HGNC:4853
Homologene: 68187
Zfyve1
Name: zinc finger, FYVE domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217695
VEGA: 12
Homologene: 10945
Kif23
Name: kinesin family member 23
Synonyms: CHO1, MKLP-1, MKLP1, 3110001D19Rik, Knsl5, C87313
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 71819
VEGA: 9
HGNC: HGNC:6392
Homologene: 11491
Arhgap23
Name: Rho GTPase activating protein 23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 58996
Homologene: 104145
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 2310042E16Rik, Coh1, 1810042B05Rik, C330002D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Chn2
Name: chimerin 2
Synonyms: 4930557O16Rik, 1700026N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 69993
HGNC: HGNC:1944
Homologene: 31213
Tpd52l1
Name: tumor protein D52-like 1
Synonyms: D53
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 21987
VEGA: 10
Homologene: 2468
St14
Name: suppression of tumorigenicity 14 (colon carcinoma)
Synonyms: Tmprss14, Prss14, Epithin, matriptase, MT-SP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 19143
Homologene: 7906
Lrch3
Name: leucine-rich repeats and calponin homology (CH) domain containing 3
Synonyms: 2210409B11Rik, LOC385628
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 70144
Homologene: 13111
Garnl3
Name: GTPase activating RANGAP domain-like 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 99326
Homologene: 13003
Siglecg
Name: sialic acid binding Ig-like lectin G
Synonyms: mSiglec-G, A630096C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 243958
Homologene: 13228
Abca16
Name: ATP-binding cassette, sub-family A (ABC1), member 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233810
Homologene: 132942
Slc9a1
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 1
Synonyms: Apnh, Nhe1, antiporter, Nhe-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 20544
Homologene: 20660
Skor2
Name: SKI family transcriptional corepressor 2
Synonyms: Corl2, Gm7348, Fussel18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 664805
VEGA: 18
Homologene: 64797
Prickle1
Name: prickle planar cell polarity protein 1
Synonyms: 1110058P22Rik, b2b019Clo, mpk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 106042
Homologene: 17686
Itgae
Name: integrin alpha E, epithelial-associated
Synonyms: alpha-E1, CD103
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 16407
HGNC: HGNC:6147
Homologene: 113560
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Miox
Name: myo-inositol oxygenase
Synonyms: RSOR, C85427, Aldrl6, 0610009I10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 56727
Homologene: 9732
AW549877
Name: expressed sequence AW549877
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 106064
VEGA: 15
Homologene: 27085
Col5a2
Name: collagen, type V, alpha 2
Synonyms: 1110014L14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12832
HGNC: HGNC:2210
Homologene: 20119
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Vmn1r58
Name: vomeronasal 1 receptor 58
Synonyms: V1rd4, V3R4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 81014
Homologene: 41799
Gata3
Name: GATA binding protein 3
Synonyms: jal, Gata-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 14462
HGNC: HGNC:4172
Homologene: 1550
Mndal
Name: myeloid nuclear differentiation antigen like
Synonyms: Ifi212
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 100040462
HGNC: HGNC:5395
Homologene: 115929
Fam234b
Name: family with sequence similarity 234, member B
Synonyms: 8430419L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 74525
Homologene: 12572
Olfr729
Name: olfactory receptor 729
Synonyms: MOR246-6, GA_x6K02T2PMLR-5839874-5838903
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 258275
Homologene: 17167
4931417E11Rik
Name: RIKEN cDNA 4931417E11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 66740
Homologene: 75225
Gm10647
Name: predicted gene 10647
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
Zyg11a
Name: zyg-11 family member A, cell cycle regulator
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230590
Homologene: 66294
Nepn
Name: nephrocan
Synonyms: Npn, 5730521E12Rik, periolin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 66650
Homologene: 23549
Tas2r120
Name: taste receptor, type 2, member 120
Synonyms: mt2r47, T2R20, Tas2r20, mGR20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 387348
Homologene: 135705
Mug2
Name: murinoglobulin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 17837
Homologene: 136663
Dnlz
Name: DNL-type zinc finger
Synonyms: D2Bwg1335e, 1110034G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 52838
Homologene: 36864
Olfr1055
Name: olfactory receptor 1055
Synonyms: MOR186-1, GA_x6K02T2Q125-47819205-47818258
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 259023
Homologene: 131140
Cd59b
Name: CD59b antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 333883
HGNC: HGNC:1689
Homologene: 56386
Plekhn1
Name: pleckstrin homology domain containing, family N member 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 231002
VEGA: 4
Homologene: 12949
Vmn2r13
Name: vomeronasal 2, receptor 13
Synonyms: Gm4867
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231589
Homologene: 129606
Vmn1r45
Name: vomeronasal 1 receptor 45
Synonyms: V1r2, V1ra2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 22297
Homologene: 130651
Olfr307
Name: olfactory receptor 307
Synonyms: MOR219-1, GA_x6K02T2NHDJ-9772012-9772953
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258610
Kctd21
Name: potassium channel tetramerisation domain containing 21
Synonyms: EG622320
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 622320
Homologene: 54754
Abraxas2
Name: BRISC complex subunit
Synonyms: Fam175b, C430003P19Rik, KIAA0157
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 109359
Homologene: 12970
Vmn2r92
Name: vomeronasal 2, receptor 92
Synonyms: EG627111
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 627111
Homologene: 115024
Map9
Name: microtubule-associated protein 9
Synonyms: ASAP, 5033421J10Rik, 5330427D05Rik, Mtap9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 213582
Homologene: 41591
Ighv15-2
Name: immunoglobulin heavy variable V15-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 780864
Dele1
Name: DAP3 binding cell death enhancer 1
Synonyms: 0610009O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 66839
VEGA: 18
Homologene: 32232
Ptar1
Name: protein prenyltransferase alpha subunit repeat containing 1
Synonyms: 2010013L17Rik, 4930428J16Rik, 1700084D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 72351
VEGA: 19
Homologene: 34254
Lipo4
Name: lipase, member O4
Synonyms: Gm6857
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 628236
Homologene: 103863
Zfp948
Name: zinc finger protein 948
Synonyms: BC049807
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 381066
VEGA: 17
Wfdc8
Name: WAP four-disulfide core domain 8
Synonyms: LOC277343
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 277343
Homologene: 33799
Tyms
Name: thymidylate synthase
Synonyms: TS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 22171
Homologene: 834
F11
Name: coagulation factor XI
Synonyms: plasma thromboplastin antecedent, Cf11, 1600027G01Rik, FXI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 109821
HGNC: HGNC:3529
Homologene: 86654
Olfr933
Name: olfactory receptor 933
Synonyms: GA_x6K02T2PVTD-32671531-32672457, MOR171-22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258433
VEGA: 9
HGNC: HGNC:8481
Homologene: 79676
Gadd45b
Name: growth arrest and DNA-damage-inducible 45 beta
Synonyms: Myd118
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 17873
VEGA: 10
HGNC: HGNC:4096
Homologene: 7433
Tbxa2r
Name: thromboxane A2 receptor
Synonyms: Tp receptor, TP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 21390
VEGA: 10
Homologene: 825
Gm10936
Name: predicted gene 10936
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
Gzmd
Name: granzyme D
Synonyms: Ctla5, CCP5, Ctla-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 14941
HGNC: HGNC:4710
Homologene: 133275
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 30,805,065 bp
  • A to G, chromosome 1 at 45,407,035 bp
  • A to T, chromosome 1 at 138,838,679 bp
  • A to T, chromosome 1 at 173,857,513 bp
  • T to C, chromosome 2 at 9,874,809 bp
  • T to C, chromosome 2 at 26,351,368 bp
  • T to C, chromosome 2 at 33,006,804 bp
  • T to C, chromosome 2 at 40,596,983 bp
  • T to A, chromosome 2 at 76,710,124 bp
  • C to A, chromosome 2 at 76,793,130 bp
  • T to C, chromosome 2 at 86,347,728 bp
  • G to A, chromosome 2 at 104,078,941 bp
  • A to G, chromosome 2 at 164,597,185 bp
  • A to T, chromosome 3 at 79,104,105 bp
  • G to A, chromosome 3 at 82,359,983 bp
  • G to A, chromosome 4 at 108,204,641 bp
  • A to G, chromosome 4 at 133,420,605 bp
  • T to C, chromosome 4 at 156,228,243 bp
  • A to G, chromosome 5 at 30,063,258 bp
  • C to A, chromosome 5 at 109,175,049 bp
  • T to C, chromosome 6 at 54,290,451 bp
  • A to G, chromosome 6 at 73,469,419 bp
  • A to T, chromosome 6 at 89,933,510 bp
  • G to T, chromosome 6 at 122,074,714 bp
  • T to A, chromosome 6 at 132,657,589 bp
  • T to G, chromosome 6 at 135,217,074 bp
  • T to C, chromosome 6 at 149,512,950 bp
  • A to C, chromosome 7 at 5,410,866 bp
  • G to A, chromosome 7 at 43,411,171 bp
  • A to G, chromosome 7 at 86,335,595 bp
  • T to A, chromosome 7 at 97,348,091 bp
  • T to A, chromosome 7 at 120,540,155 bp
  • G to A, chromosome 7 at 132,874,855 bp
  • T to C, chromosome 8 at 45,249,077 bp
  • T to A, chromosome 9 at 31,108,284 bp
  • A to G, chromosome 9 at 38,976,278 bp
  • C to G, chromosome 9 at 61,925,694 bp
  • A to G, chromosome 9 at 66,798,495 bp
  • A to G, chromosome 10 at 31,379,256 bp
  • T to A, chromosome 10 at 36,989,184 bp
  • A to T, chromosome 10 at 52,400,437 bp
  • T to C, chromosome 10 at 77,088,914 bp
  • A to G, chromosome 10 at 80,930,347 bp
  • C to A, chromosome 10 at 81,332,900 bp
  • G to A, chromosome 10 at 117,248,130 bp
  • A to G, chromosome 11 at 54,265,680 bp
  • A to C, chromosome 11 at 73,111,342 bp
  • G to T, chromosome 11 at 97,444,328 bp
  • A to G, chromosome 12 at 83,555,073 bp
  • T to G, chromosome 12 at 114,565,037 bp
  • A to G, chromosome 12 at 116,367,710 bp
  • T to G, chromosome 14 at 50,148,635 bp
  • A to G, chromosome 14 at 56,129,704 bp
  • A to T, chromosome 14 at 70,532,821 bp
  • T to C, chromosome 15 at 3,986,294 bp
  • T to C, chromosome 15 at 35,887,261 bp
  • C to T, chromosome 15 at 89,334,454 bp
  • A to G, chromosome 15 at 93,510,777 bp
  • T to A, chromosome 16 at 18,956,171 bp
  • T to A, chromosome 16 at 32,961,754 bp
  • C to T, chromosome 17 at 18,167,957 bp
  • A to T, chromosome 17 at 21,587,294 bp
  • A to G, chromosome 17 at 53,641,210 bp
  • G to A, chromosome 18 at 38,261,264 bp
  • A to T, chromosome 18 at 76,859,098 bp
  • T to A, chromosome 19 at 23,718,095 bp
  • A to G, chromosome 19 at 33,501,606 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0225 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038470-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.