Strain Name:
C57BL/6J-MtgxR0234Btlr/Mmmh
Stock Number:
038475-MU
Citation ID:
RRID:MMRRC_038475-MU
Other Names:
R0234 (G1), C57BL/6J-MtgxR0234Btlr
Major Collection:

Gene Information

Gene Symbol: Spry4 [MGI:1345144] (Mus musculus (mouse))
Name: sprouty RTK signaling antagonist 4
Synonyms: sprouty4, A030006O18Rik
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Ubr5 [MGI:1918040] (Mus musculus (mouse))
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: Edd1, Edd, 4432411E13Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Agap1 [MGI:2653690] (Mus musculus (mouse))
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Synonyms: Centg2, Ggap1
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Ccn1 [MGI:88613] (Mus musculus (mouse))
Name: cellular communication network factor 1
Synonyms: CCN1, Cyr61
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Tead4 [MGI:106907] (Mus musculus (mouse))
Name: TEA domain family member 4
Synonyms: Rtef1, TEAD-4, Tefr1a, Tcf13r1, Tefr, Tef3, Etfr2, TEF-3, ETFR-2a
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Plcb4 [MGI:107464] (Mus musculus (mouse))
Name: phospholipase C, beta 4
Synonyms: A930039J07Rik, C230058B11Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Itsn1 [MGI:1338069] (Mus musculus (mouse))
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: EHSH1, Intersectin-L, Ese1, Sh3p17, Eh domain, SH3 domain regulator of endocytosis 1
Chromosome: 16
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 75,529,067 bp
  • A to G, chromosome 1 at 89,671,212 bp
  • T to A, chromosome 1 at 160,088,466 bp
  • T to C, chromosome 1 at 191,162,908 bp
  • A to G, chromosome 2 at 14,279,894 bp
  • G to A, chromosome 2 at 32,758,704 bp
  • T to A, chromosome 2 at 88,093,022 bp
  • T to C, chromosome 2 at 111,983,300 bp
  • T to A, chromosome 2 at 121,148,317 bp
  • A to G, chromosome 2 at 129,463,464 bp
  • T to C, chromosome 2 at 129,615,468 bp
  • T to C, chromosome 2 at 135,982,075 bp
  • T to A, chromosome 2 at 162,961,502 bp
  • A to T, chromosome 2 at 181,864,579 bp
  • T to C, chromosome 3 at 107,624,526 bp
  • A to G, chromosome 3 at 132,914,414 bp
  • A to G, chromosome 3 at 145,647,981 bp
  • T to C, chromosome 4 at 43,543,811 bp
  • G to T, chromosome 4 at 109,056,506 bp
  • A to G, chromosome 4 at 128,767,148 bp
  • T to C, chromosome 4 at 152,112,219 bp
  • A to C, chromosome 5 at 89,156,336 bp
  • A to G, chromosome 5 at 93,202,327 bp
  • GGAAGAAGAAGAAGAAGA to GGAAGAAGAAGAAGA, chromosome 5 at 104,310,069 bp
  • A to T, chromosome 5 at 114,209,817 bp
  • T to C, chromosome 6 at 33,862,087 bp
  • A to T, chromosome 6 at 48,438,755 bp
  • T to G, chromosome 6 at 54,496,501 bp
  • A to T, chromosome 6 at 55,379,186 bp
  • T to C, chromosome 6 at 122,456,333 bp
  • T to A, chromosome 6 at 124,231,619 bp
  • A to C, chromosome 6 at 125,312,873 bp
  • C to T, chromosome 6 at 128,243,402 bp
  • T to A, chromosome 7 at 10,381,608 bp
  • C to A, chromosome 7 at 24,433,328 bp
  • G to A, chromosome 7 at 24,612,579 bp
  • T to A, chromosome 7 at 29,894,107 bp
  • T to C, chromosome 7 at 34,365,617 bp
  • T to A, chromosome 7 at 97,714,032 bp
  • T to C, chromosome 7 at 105,144,074 bp
  • C to A, chromosome 7 at 105,203,614 bp
  • T to C, chromosome 7 at 125,795,385 bp
  • T to A, chromosome 7 at 131,194,303 bp
  • A to C, chromosome 7 at 141,649,674 bp
  • T to A, chromosome 8 at 31,236,197 bp
  • T to A, chromosome 8 at 35,384,501 bp
  • A to T, chromosome 8 at 70,889,633 bp
  • A to G, chromosome 9 at 26,756,081 bp
  • A to C, chromosome 9 at 38,976,251 bp
  • A to T, chromosome 9 at 90,009,366 bp
  • T to C, chromosome 9 at 103,226,879 bp
  • T to C, chromosome 9 at 106,885,364 bp
  • T to C, chromosome 9 at 108,116,396 bp
  • C to T, chromosome 10 at 42,798,798 bp
  • T to A, chromosome 10 at 86,448,886 bp
  • A to T, chromosome 10 at 92,899,160 bp
  • A to T, chromosome 10 at 100,450,322 bp
  • A to T, chromosome 11 at 65,187,240 bp
  • C to G, chromosome 11 at 72,250,800 bp
  • C to T, chromosome 11 at 90,644,500 bp
  • T to C, chromosome 11 at 98,436,439 bp
  • A to C, chromosome 11 at 116,155,034 bp
  • T to C, chromosome 11 at 120,598,307 bp
  • G to A, chromosome 12 at 113,490,610 bp
  • C to A, chromosome 13 at 6,575,079 bp
  • T to C, chromosome 13 at 23,739,123 bp
  • C to T, chromosome 13 at 23,855,858 bp
  • G to A, chromosome 13 at 34,183,261 bp
  • A to G, chromosome 13 at 38,187,893 bp
  • G to C, chromosome 13 at 46,638,022 bp
  • A to G, chromosome 13 at 99,234,260 bp
  • A to T, chromosome 14 at 33,955,901 bp
  • T to A, chromosome 14 at 63,183,526 bp
  • A to C, chromosome 15 at 34,488,560 bp
  • T to C, chromosome 15 at 37,968,493 bp
  • T to A, chromosome 15 at 84,703,121 bp
  • T to C, chromosome 16 at 33,066,324 bp
  • A to T, chromosome 16 at 91,828,280 bp
  • T to C, chromosome 17 at 15,373,708 bp
  • T to A, chromosome 17 at 37,660,106 bp
  • C to T, chromosome 17 at 46,240,155 bp
  • T to A, chromosome 18 at 10,560,331 bp
  • C to T, chromosome 18 at 24,254,633 bp
  • A to G, chromosome 18 at 38,590,089 bp
  • T to C, chromosome 18 at 82,629,994 bp
  • T to C, chromosome 19 at 5,672,618 bp
  • C to A, chromosome 19 at 7,926,791 bp
  • C to T, chromosome 19 at 13,838,538 bp
  • A to G, chromosome 19 at 41,871,760 bp
  • T to C, chromosome 19 at 42,762,553 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0234 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038475-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.