Strain Name:
C57BL/6J-MtgxR0314Btlr/Mmmh
Stock Number:
038524-MU
Citation ID:
RRID:MMRRC_038524-MU
Other Names:
R0314 (G1), C57BL/6J-MtgxR0314Btlr
Major Collection:

Gene Information

Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: 4432411E13Rik, Edd1, Edd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 70790
VEGA: 15
Homologene: 9295
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Fzd6
Name: frizzled class receptor 6
Synonyms: Fz6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 14368
VEGA: 15
HGNC: HGNC:4044
Homologene: 2617
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, B630009I04Rik, Helic1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Cenpc1
Name: centromere protein C1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12617
HGNC: HGNC:1854
Homologene: 1371
Lgr4
Name: leucine-rich repeat-containing G protein-coupled receptor 4
Synonyms: A930009A08Rik, Gpr48, A330106J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 107515
Homologene: 10226
Rif1
Name: replication timing regulatory factor 1
Synonyms: 5730435J01Rik, 6530403D07Rik, D2Ertd145e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 51869
Homologene: 41231
Robo2
Name: roundabout guidance receptor 2
Synonyms: 9430089E08Rik, D230004I22Rik, 2600013A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 268902
Homologene: 43188
Uba6
Name: ubiquitin-like modifier activating enzyme 6
Synonyms: 5730469D23Rik, Ube1l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231380
Homologene: 10080
Zzz3
Name: zinc finger, ZZ domain containing 3
Synonyms: 3110065C23Rik, 6430567E01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 108946
Homologene: 9182
Arih2
Name: ariadne RBR E3 ubiquitin protein ligase 2
Synonyms: TRIAD1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 23807
HGNC: HGNC:690
Homologene: 48424
Zdhhc20
Name: zinc finger, DHHC domain containing 20
Synonyms: B230110O18Rik, 5033406L14Rik, ENSMUSG00000055956, 4930542A17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 75965
VEGA: 14
Homologene: 57105
Car9
Name: carbonic anhydrase 9
Synonyms: CAIX, MN/CA9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230099
HGNC: HGNC:1383
Homologene: 20325
Limd1
Name: LIM domains containing 1
Synonyms: D9Ertd192e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 29806
VEGA: 9
HGNC: HGNC:6612
Homologene: 8478
Cntn3
Name: contactin 3
Synonyms: Pang
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 18488
HGNC: HGNC:2173
Homologene: 7461
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: 4833413O10Rik, D430029O09Rik, 1110021D17Rik, Polydom
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 64817
Homologene: 23386
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Vmn2r1
Name: vomeronasal 2, receptor 1
Synonyms: EG56544, V2r83
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56544
Homologene: 84832
Chl1
Name: cell adhesion molecule L1-like
Synonyms: CALL, A530023M13Rik, close homolog of L1, LICAM2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12661
HGNC: HGNC:1939
Homologene: 21314
Rbbp8
Name: retinoblastoma binding protein 8, endonuclease
Synonyms: 9930104E21Rik, CtIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225182
VEGA: 18
HGNC: HGNC:9891
Homologene: 28546
Grm5
Name: glutamate receptor, metabotropic 5
Synonyms: mGluR5, 6430542K11Rik, Gprc1e, Glu5R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 108071
HGNC: HGNC:4597
Homologene: 37354
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: Cav2.3, Cchra1, alpha1E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Spag4
Name: sperm associated antigen 4
Synonyms: 1700041K21Rik, Sun4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 245865
Homologene: 2343
Vstm2a
Name: V-set and transmembrane domain containing 2A
Synonyms: 9330184N17Rik, Vstm2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 211739
Homologene: 17123
Slc5a1
Name: solute carrier family 5 (sodium/glucose cotransporter), member 1
Synonyms: Sglt1, sodium glucose cotransporter 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20537
Homologene: 55456
Fkbpl
Name: FK506 binding protein-like
Synonyms: Ppiase-X, NG7, DIR1, WISp39, WAF-1/CIP1 stabilizing protein 39
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 56299
Homologene: 10529
Fmo1
Name: flavin containing monooxygenase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14261
HGNC: HGNC:3769
Homologene: 55520
Vmn2r60
Name: vomeronasal 2, receptor 60
Synonyms: Casr-rs3, EG637898, Gprc2a-rs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 637898
Homologene: 129683
Cobll1
Name: Cobl-like 1
Synonyms: Coblr1, D430044D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 319876
Homologene: 8933
Fnip2
Name: folliculin interacting protein 2
Synonyms: D630023B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329679
Homologene: 46417
Ube2j1
Name: ubiquitin-conjugating enzyme E2J 1
Synonyms: 0710008M05Rik, Ncube, Ubc6p, 1110030I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56228
Homologene: 41090
Zfp683
Name: zinc finger protein 683
Synonyms: Hobit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100503878
Pex26
Name: peroxisomal biogenesis factor 26
Synonyms: 4632428M11Rik, peroxisome biogenesis factor 26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74043
Homologene: 9922
Klra5
Name: killer cell lectin-like receptor, subfamily A, member 5
Synonyms: Ly49e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16636
Homologene: 110821
Vmn1r113
Name: vomeronasal 1 receptor 113
Synonyms: Gm5748
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 436135
Homologene: 104166
Or52a5b
Name: olfactory receptor family 52 subfamily A member 5B
Synonyms: 3'[b]3, MOR22-2, GA_x6K02T2PBJ9-6494485-6493535, Olfr69
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18370
Homologene: 128070
Stt3a
Name: STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Synonyms: Itm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16430
HGNC: HGNC:6172
Homologene: 40617
Gm12169
Name: predicted gene 12169
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 210535
Homologene: 130033
Or2v2
Name: olfactory receptor family 2 subfamily V member 2
Synonyms: GA_x6K02T2QP88-6321048-6321995, MOR276-2, Olfr1396
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258334
Homologene: 28537
Pebp4
Name: phosphatidylethanolamine binding protein 4
Synonyms: 1700051A02Rik, 1700081D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 73523
VEGA: 14
Homologene: 51650
Mpv17l
Name: Mpv17 transgene, kidney disease mutant-like
Synonyms: M-LP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 93734
Homologene: 13547
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 154,442,251 bp
  • T to G, chromosome 1 at 162,859,462 bp
  • GCCACCA to GCCA, chromosome 2 at 52,110,324 bp
  • A to T, chromosome 2 at 52,243,331 bp
  • T to C, chromosome 2 at 65,089,521 bp
  • T to C, chromosome 2 at 109,991,093 bp
  • C to T, chromosome 2 at 156,067,309 bp
  • A to G, chromosome 3 at 64,086,559 bp
  • G to A, chromosome 3 at 79,481,189 bp
  • T to C, chromosome 3 at 152,427,448 bp
  • T to A, chromosome 4 at 33,043,991 bp
  • T to C, chromosome 4 at 43,509,212 bp
  • C to T, chromosome 4 at 58,096,331 bp
  • A to G, chromosome 4 at 134,058,741 bp
  • T to C, chromosome 5 at 33,146,651 bp
  • A to T, chromosome 5 at 86,037,371 bp
  • A to T, chromosome 5 at 86,118,087 bp
  • T to C, chromosome 6 at 102,420,381 bp
  • T to A, chromosome 6 at 103,647,301 bp
  • T to C, chromosome 6 at 121,184,484 bp
  • T to C, chromosome 6 at 129,903,590 bp
  • C to T, chromosome 7 at 20,787,950 bp
  • T to C, chromosome 7 at 42,135,561 bp
  • T to C, chromosome 7 at 87,602,955 bp
  • T to C, chromosome 7 at 103,768,181 bp
  • T to C, chromosome 9 at 36,749,545 bp
  • T to C, chromosome 9 at 108,608,679 bp
  • T to G, chromosome 9 at 123,516,827 bp
  • C to T, chromosome 10 at 50,637,999 bp
  • A to G, chromosome 11 at 16,368,388 bp
  • G to A, chromosome 11 at 46,528,537 bp
  • A to T, chromosome 11 at 49,113,692 bp
  • T to A, chromosome 14 at 57,856,619 bp
  • G to T, chromosome 14 at 70,059,654 bp
  • T to C, chromosome 15 at 8,147,252 bp
  • A to G, chromosome 15 at 37,997,187 bp
  • T to A, chromosome 15 at 39,025,733 bp
  • A to T, chromosome 16 at 13,940,999 bp
  • G to A, chromosome 16 at 73,956,637 bp
  • C to T, chromosome 17 at 34,646,052 bp
  • A to T, chromosome 18 at 11,715,818 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0314 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038524-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.