Strain Name:
C57BL/6J-MtgxR0329Btlr/Mmmh
Stock Number:
038538-MU
Citation ID:
RRID:MMRRC_038538-MU
Other Names:
R0329 (G1), C57BL/6J-MtgxR0329Btlr
Major Collection:

Gene Information

Gene Symbol: Kit [MGI:96677] (Mus musculus (mouse))
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: SCO5, SCO1, Dominant white spotting, Tr-kit, CD117, belly-spot, c-KIT, Steel Factor Receptor, Gsfsco1, Gsfsco5, Gsfsow3, SOW3
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Acvr1c [MGI:2661081] (Mus musculus (mouse))
Name: activin A receptor, type IC
Synonyms: Alk-7, ALK7
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Erbb4 [MGI:104771] (Mus musculus (mouse))
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: Her4, ErbB4
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Tfdp2 [MGI:107167] (Mus musculus (mouse))
Name: transcription factor Dp 2
Synonyms: A330080J22Rik, 1110029I05Rik, DP3, DP3, DP-3
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Utp20 [MGI:1917933] (Mus musculus (mouse))
Name: UTP20 small subunit processome component
Synonyms: 3830408P06Rik, mDRIM, DRIM
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Pcif1 [MGI:2443858] (Mus musculus (mouse))
Name: PDX1 C-terminal inhibiting factor 1
Synonyms: F730014I05Rik, 2310022K11Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Tsacc [MGI:1924177] (Mus musculus (mouse))
Name: TSSK6 activating co-chaperone
Synonyms: SIP, 1700021C14Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 43,111,117 bp
  • A to G, chromosome 1 at 52,090,870 bp
  • A to G, chromosome 1 at 60,268,063 bp
  • T to C, chromosome 1 at 66,674,087 bp
  • T to C, chromosome 1 at 68,298,280 bp
  • G to T, chromosome 1 at 107,159,703 bp
  • G to C, chromosome 1 at 136,496,026 bp
  • T to A, chromosome 1 at 172,187,411 bp
  • T to C, chromosome 2 at 58,284,838 bp
  • T to C, chromosome 2 at 71,895,674 bp
  • T to A, chromosome 2 at 85,518,551 bp
  • T to A, chromosome 2 at 85,785,803 bp
  • A to G, chromosome 2 at 103,430,420 bp
  • A to T, chromosome 2 at 120,338,787 bp
  • A to G, chromosome 2 at 132,819,994 bp
  • T to C, chromosome 2 at 160,905,305 bp
  • G to T, chromosome 2 at 164,889,444 bp
  • T to C, chromosome 3 at 30,655,724 bp
  • C to A, chromosome 3 at 64,691,018 bp
  • T to C, chromosome 3 at 79,609,844 bp
  • A to G, chromosome 3 at 88,282,862 bp
  • C to T, chromosome 3 at 135,373,235 bp
  • T to A, chromosome 3 at 137,416,814 bp
  • T to G, chromosome 3 at 142,608,176 bp
  • A to G, chromosome 4 at 43,030,238 bp
  • C to A, chromosome 4 at 118,484,727 bp
  • T to A, chromosome 4 at 148,484,380 bp
  • T to C, chromosome 5 at 7,975,829 bp
  • T to C, chromosome 5 at 24,545,785 bp
  • T to A, chromosome 5 at 34,817,134 bp
  • T to A, chromosome 5 at 67,812,073 bp
  • T to G, chromosome 5 at 75,652,829 bp
  • A to G, chromosome 5 at 87,003,405 bp
  • T to C, chromosome 5 at 121,259,864 bp
  • T to C, chromosome 5 at 124,559,147 bp
  • C to A, chromosome 5 at 130,042,242 bp
  • T to A, chromosome 6 at 18,226,097 bp
  • A to G, chromosome 6 at 25,778,831 bp
  • T to C, chromosome 6 at 29,711,092 bp
  • T to C, chromosome 6 at 65,862,903 bp
  • T to A, chromosome 6 at 71,966,416 bp
  • A to G, chromosome 6 at 142,361,976 bp
  • A to G, chromosome 7 at 25,308,674 bp
  • T to C, chromosome 7 at 31,138,254 bp
  • T to A, chromosome 7 at 36,770,033 bp
  • C to T, chromosome 7 at 44,509,029 bp
  • C to T, chromosome 7 at 79,838,256 bp
  • A to T, chromosome 7 at 82,521,990 bp
  • A to T, chromosome 7 at 141,439,561 bp
  • A to T, chromosome 8 at 46,610,461 bp
  • G to T, chromosome 8 at 69,810,818 bp
  • C to A, chromosome 8 at 105,483,543 bp
  • T to A, chromosome 8 at 105,876,363 bp
  • T to C, chromosome 8 at 106,010,245 bp
  • T to C, chromosome 8 at 119,596,017 bp
  • T to A, chromosome 8 at 119,936,557 bp
  • T to A, chromosome 9 at 5,345,534 bp
  • T to A, chromosome 9 at 20,285,857 bp
  • C to T, chromosome 9 at 20,638,480 bp
  • T to C, chromosome 9 at 39,470,556 bp
  • T to C, chromosome 9 at 57,141,183 bp
  • C to G, chromosome 9 at 59,923,677 bp
  • T to G, chromosome 9 at 96,306,893 bp
  • C to A, chromosome 9 at 118,014,055 bp
  • T to C, chromosome 9 at 123,048,557 bp
  • C to T, chromosome 10 at 75,181,634 bp
  • T to C, chromosome 10 at 86,694,155 bp
  • T to C, chromosome 10 at 88,440,309 bp
  • T to A, chromosome 10 at 88,817,979 bp
  • T to C, chromosome 10 at 99,126,117 bp
  • A to T, chromosome 11 at 9,399,430 bp
  • T to A, chromosome 11 at 59,040,441 bp
  • A to T, chromosome 11 at 59,052,506 bp
  • A to G, chromosome 11 at 64,979,310 bp
  • A to G, chromosome 11 at 70,640,409 bp
  • A to T, chromosome 11 at 101,188,309 bp
  • C to T, chromosome 12 at 36,381,838 bp
  • C to A, chromosome 12 at 69,197,744 bp
  • G to T, chromosome 12 at 75,966,953 bp
  • T to G, chromosome 13 at 15,723,558 bp
  • T to A, chromosome 13 at 54,734,801 bp
  • G to T, chromosome 13 at 67,620,354 bp
  • T to A, chromosome 13 at 77,761,951 bp
  • T to A, chromosome 13 at 91,680,579 bp
  • T to A, chromosome 13 at 91,863,203 bp
  • C to A, chromosome 13 at 103,868,865 bp
  • T to A, chromosome 13 at 112,989,996 bp
  • A to C, chromosome 14 at 20,469,498 bp
  • A to G, chromosome 14 at 33,369,417 bp
  • C to T, chromosome 14 at 34,429,777 bp
  • T to C, chromosome 14 at 68,617,739 bp
  • A to G, chromosome 15 at 76,191,418 bp
  • A to T, chromosome 15 at 102,715,763 bp
  • A to T, chromosome 16 at 75,765,130 bp
  • A to G, chromosome 17 at 14,934,459 bp
  • A to C, chromosome 17 at 19,066,347 bp
  • A to T, chromosome 17 at 29,211,660 bp
  • A to T, chromosome 17 at 37,795,989 bp
  • A to T, chromosome 17 at 42,667,313 bp
  • A to G, chromosome 17 at 43,497,898 bp
  • G to T, chromosome 17 at 55,610,717 bp
  • A to T, chromosome 17 at 56,883,631 bp
  • A to G, chromosome 18 at 13,007,245 bp
  • A to G, chromosome 18 at 37,267,024 bp
  • A to G, chromosome 18 at 44,865,060 bp
  • C to T, chromosome 19 at 43,441,910 bp
  • A to G, chromosome 19 at 45,749,526 bp
  • G to A, chromosome 19 at 46,272,270 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0329 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038538-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.