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Strain Name:
C57BL/6J-MtgxR0373Btlr/Mmmh
Stock Number:
038579-MU
Citation ID:
RRID:MMRRC_038579-MU
Other Names:
R0373 (G1), C57BL/6J-MtgxR0373Btlr
Major Collection:

Strain Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Btrc
Name: beta-transducin repeat containing protein
Synonyms: SCF b-TRCP, beta-TrCP, Slimb, Beta-Trcp1, Fbw1a
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12234
VEGA: 19
HGNC: HGNC:1144
Homologene: 39330
Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12322
HGNC: HGNC:1460
Homologene: 56577
Sema3b
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Synonyms: SemA, semaV, sema5, SemA, LUCA-1, Semaa
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20347
Homologene: 20972
Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Rubcn
Name: RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein
Synonyms: 1700021K19Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 100502698
Homologene: 15687
Epha7
Name: Eph receptor A7
Synonyms: MDK1, Ebk, Cek11, Ehk3, Hek11, Mdk1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13841
HGNC: HGNC:3390
Homologene: 20935
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 36,179,670 bp
  • T to C, chromosome 1 at 52,188,699 bp
  • T to C, chromosome 1 at 74,633,620 bp
  • C to T, chromosome 1 at 92,896,190 bp
  • A to T, chromosome 1 at 97,740,537 bp
  • T to G, chromosome 2 at 5,911,870 bp
  • G to T, chromosome 2 at 30,330,988 bp
  • A to T, chromosome 2 at 32,367,467 bp
  • A to T, chromosome 2 at 66,188,326 bp
  • A to G, chromosome 2 at 74,548,065 bp
  • A to C, chromosome 2 at 86,171,706 bp
  • A to T, chromosome 2 at 89,170,413 bp
  • T to C, chromosome 2 at 105,005,000 bp
  • C to T, chromosome 2 at 119,293,905 bp
  • A to T, chromosome 2 at 120,946,657 bp
  • A to T, chromosome 2 at 122,689,399 bp
  • A to T, chromosome 2 at 125,226,031 bp
  • A to G, chromosome 2 at 140,170,881 bp
  • A to G, chromosome 2 at 160,909,817 bp
  • T to A, chromosome 2 at 173,153,390 bp
  • T to A, chromosome 3 at 88,382,129 bp
  • T to C, chromosome 3 at 115,122,805 bp
  • A to T, chromosome 3 at 138,173,582 bp
  • T to C, chromosome 4 at 24,682,048 bp
  • A to G, chromosome 4 at 28,935,700 bp
  • T to C, chromosome 4 at 42,851,161 bp
  • T to A, chromosome 4 at 61,856,149 bp
  • A to T, chromosome 4 at 94,804,341 bp
  • A to T, chromosome 4 at 130,950,106 bp
  • A to T, chromosome 4 at 144,686,220 bp
  • A to T, chromosome 4 at 155,656,288 bp
  • T to A, chromosome 5 at 21,319,545 bp
  • C to T, chromosome 5 at 129,844,818 bp
  • A to G, chromosome 5 at 143,684,426 bp
  • T to A, chromosome 6 at 113,398,777 bp
  • C to T, chromosome 6 at 148,775,042 bp
  • T to C, chromosome 7 at 35,427,795 bp
  • T to C, chromosome 7 at 35,638,053 bp
  • T to C, chromosome 7 at 43,299,336 bp
  • C to T, chromosome 7 at 45,409,444 bp
  • T to A, chromosome 7 at 45,635,244 bp
  • T to C, chromosome 7 at 75,609,929 bp
  • T to A, chromosome 7 at 75,730,500 bp
  • T to C, chromosome 7 at 80,326,344 bp
  • A to T, chromosome 7 at 86,363,805 bp
  • T to C, chromosome 7 at 92,661,215 bp
  • T to C, chromosome 7 at 104,265,684 bp
  • A to G, chromosome 8 at 13,779,264 bp
  • G to T, chromosome 8 at 15,098,419 bp
  • G to T, chromosome 8 at 41,276,111 bp
  • T to C, chromosome 8 at 93,331,193 bp
  • T to A, chromosome 8 at 94,851,839 bp
  • T to C, chromosome 8 at 110,030,394 bp
  • C to A, chromosome 8 at 125,464,410 bp
  • C to A, chromosome 9 at 14,751,210 bp
  • A to G, chromosome 9 at 28,813,398 bp
  • A to T, chromosome 9 at 86,574,400 bp
  • G to A, chromosome 9 at 87,027,040 bp
  • C to T, chromosome 9 at 95,484,443 bp
  • A to T, chromosome 9 at 99,094,431 bp
  • T to C, chromosome 9 at 107,602,918 bp
  • C to A, chromosome 10 at 60,778,940 bp
  • A to T, chromosome 10 at 62,878,209 bp
  • C to T, chromosome 10 at 75,579,270 bp
  • C to A, chromosome 10 at 78,801,922 bp
  • T to A, chromosome 10 at 111,300,052 bp
  • G to T, chromosome 11 at 9,129,590 bp
  • A to G, chromosome 11 at 53,650,519 bp
  • A to G, chromosome 11 at 61,741,000 bp
  • C to G, chromosome 11 at 77,821,042 bp
  • A to T, chromosome 11 at 97,185,090 bp
  • T to C, chromosome 11 at 120,605,377 bp
  • A to T, chromosome 11 at 121,832,178 bp
  • G to A, chromosome 12 at 17,288,168 bp
  • T to C, chromosome 12 at 24,581,515 bp
  • T to C, chromosome 12 at 76,621,371 bp
  • C to T, chromosome 12 at 81,974,579 bp
  • T to A, chromosome 12 at 113,490,655 bp
  • A to T, chromosome 13 at 13,183,024 bp
  • A to T, chromosome 14 at 30,102,339 bp
  • A to G, chromosome 14 at 50,836,768 bp
  • A to T, chromosome 14 at 55,659,375 bp
  • A to T, chromosome 14 at 59,353,344 bp
  • A to G, chromosome 14 at 60,972,036 bp
  • T to A, chromosome 16 at 15,791,927 bp
  • A to T, chromosome 16 at 32,238,405 bp
  • A to G, chromosome 16 at 32,835,980 bp
  • C to T, chromosome 16 at 46,458,187 bp
  • A to G, chromosome 16 at 49,182,959 bp
  • A to G, chromosome 17 at 10,403,418 bp
  • T to C, chromosome 17 at 12,876,956 bp
  • A to G, chromosome 17 at 28,220,180 bp
  • T to C, chromosome 17 at 33,302,522 bp
  • G to A, chromosome 17 at 57,024,007 bp
  • G to T, chromosome 17 at 80,453,763 bp
  • T to C, chromosome 18 at 12,984,231 bp
  • A to C, chromosome 18 at 20,539,747 bp
  • A to T, chromosome 18 at 25,090,104 bp
  • A to G, chromosome 18 at 44,475,222 bp
  • A to G, chromosome 18 at 60,958,238 bp
  • C to T, chromosome 19 at 5,274,824 bp
  • G to A, chromosome 19 at 17,654,849 bp
  • A to G, chromosome 19 at 18,853,587 bp
  • A to G, chromosome 19 at 44,303,040 bp
  • G to A, chromosome 19 at 45,513,532 bp
  • T to C, chromosome 19 at 47,132,950 bp
  • A to T, chromosome 19 at 59,287,367 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0373 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038579-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.