Strain Name:
C57BL/6J-MtgxR0440Btlr/Mmmh
Stock Number:
038641-MU
Citation ID:
RRID:MMRRC_038641-MU
Other Names:
R0440 (G1), C57BL/6J-MtgxR0440Btlr
Major Collection:

Gene Information

App
Name: amyloid beta (A4) precursor protein
Synonyms: betaAPP, Abeta, protease nexin II, Adap, Cvap, appican, E030013M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11820
VEGA: 16
HGNC: HGNC:620
Homologene: 56379
Prdm16
Name: PR domain containing 16
Synonyms: Mel1, 5730557K01Rik, line 27, csp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70673
Homologene: 11139
Mta3
Name: metastasis associated 3
Synonyms: 1110002J22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 116871
Homologene: 14282
Zfp207
Name: zinc finger protein 207
Synonyms: Zep, 8430401D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22680
Homologene: 134624
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72313
Homologene: 103956
Enpp2
Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Synonyms: Autotaxin, Npps2, PD-Ialpha, Pdnp2, ATX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18606
HGNC: HGNC:3357
Homologene: 4526
Eml4
Name: echinoderm microtubule associated protein like 4
Synonyms: 4930443C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78798
VEGA: 17
HGNC: HGNC:1316
Homologene: 56841
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Armc9
Name: armadillo repeat containing 9
Synonyms: 5730415N24Rik, 3830422A13Rik, 4831423D23Rik, 4930438O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 78795
Homologene: 11847
Wdr59
Name: WD repeat domain 59
Synonyms: 5430401O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319481
Homologene: 38685
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107182
Homologene: 31978
Kif5b
Name: kinesin family member 5B
Synonyms: Khc, kinesin heavy chain
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 16573
HGNC: HGNC:6324
Homologene: 55829
Ccar1
Name: cell division cycle and apoptosis regulator 1
Synonyms: Carp1, 2610511G16Rik, 9430036H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67500
VEGA: 10
Homologene: 10086
Samd4b
Name: sterile alpha motif domain containing 4B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233033
Homologene: 35268
Akap9
Name: A kinase (PRKA) anchor protein (yotiao) 9
Synonyms: AKAP450, 5730481H23Rik, G1-448-15, repro12, mei2-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Cc2d1b
Name: coiled-coil and C2 domain containing 1B
Synonyms: A830039B04Rik, Freud2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 319965
Homologene: 70951
Ptn
Name: pleiotrophin
Synonyms: heparin-binding growth factor 8, HBGF-8, heparin-binding growth-associated molecule, HB-GAM, Osf-1, Osf1, HBNF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19242
HGNC: HGNC:9630
Homologene: 2117
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: 5830451P18Rik, Duplin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67772
Homologene: 72405
Pus10
Name: pseudouridylate synthase 10
Synonyms: 2810013G11Rik, 4933435A13Rik, Ccdc139
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74467
Homologene: 12566
Ass1
Name: argininosuccinate synthetase 1
Synonyms: ASS, Ass-1, fold
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11898
HGNC: HGNC:758
Homologene: 6899
Inpp5j
Name: inositol polyphosphate 5-phosphatase J
Synonyms: Pipp, Pib5pa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 170835
HGNC: HGNC:8956
Homologene: 8682
Lifr
Name: LIF receptor alpha
Synonyms: soluble differentiation-stimulating factor receptor, A230075M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16880
VEGA: 15
HGNC: HGNC:6597
Homologene: 1735
Zfp748
Name: zinc finger protein 748
Synonyms: 2610014M12Rik, mszf54, KRAB-O, Zfp208
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 212276
Homologene: 134552
Usp8
Name: ubiquitin specific peptidase 8
Synonyms: Ubpy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 84092
Homologene: 3782
Golga2
Name: golgi autoantigen, golgin subfamily a, 2
Synonyms: GM130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99412
Homologene: 3300
Dlat
Name: dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)
Synonyms: PDC-E2, 6332404G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235339
HGNC: HGNC:2896
Homologene: 6814
Plxna2
Name: plexin A2
Synonyms: OCT, Plxn2, 2810428A13Rik, PlexA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Dclk3
Name: doublecortin-like kinase 3
Synonyms: Click-I, -II related, Dcamkl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 245038
Homologene: 70580
Col13a1
Name: collagen, type XIII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12817
HGNC: HGNC:2190
Homologene: 22421
Muc5ac
Name: mucin 5, subtypes A and C, tracheobronchial/gastric
Synonyms: MGM, 2210005L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17833
HGNC: HGNC:7515
Homologene: 137237
Slc45a2
Name: solute carrier family 45, member 2
Synonyms: Dbr, bls, Aim1, Aim-1, dominant brown, blanc-sale, Matp, Oca4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22293
Homologene: 9412
Oca2
Name: oculocutaneous albinism II
Synonyms: D7H15S12, D7H15S12, p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18431
HGNC: HGNC:8101
Homologene: 37281
A4galt
Name: alpha 1,4-galactosyltransferase
Synonyms: Gb3 synthase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239559
VEGA: 15
Homologene: 9690
Stk10
Name: serine/threonine kinase 10
Synonyms: Lok, Gek1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20868
Homologene: 38122
Tubgcp6
Name: tubulin, gamma complex associated protein 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 328580
Homologene: 32477
Agl
Name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase
Synonyms: 9430004C13Rik, 9630046L06Rik, 1110061O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77559
HGNC: HGNC:321
Homologene: 536
Stab2
Name: stabilin 2
Synonyms: STAB-2, FEEL-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 192188
Homologene: 23022
Ccny
Name: cyclin Y
Synonyms: 4631402G10Rik, 3110050L10Rik, 1700025H17Rik, 5730405I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 67974
VEGA: 18
Homologene: 12219
Rp1
Name: retinitis pigmentosa 1 (human)
Synonyms: oxygen-regulated protein 1, Orp1, mG145, Dcdc3, Rp1h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19888
Homologene: 4564
Sdr9c7
Name: 4short chain dehydrogenase/reductase family 9C, member 7
Synonyms: SDR-O, Rdhs, 1810054F20Rik, Sdro, Rdh20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70061
Homologene: 23618
Synpo2l
Name: synaptopodin 2-like
Synonyms: 1110054M18Rik, Chap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 68760
Homologene: 23499
Ttc21b
Name: tetratricopeptide repeat domain 21B
Synonyms: 2410066K11Rik, line 158, Thm1, aln
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 73668
Homologene: 57006
Clstn3
Name: calsyntenin 3
Synonyms: Cst-3, CSTN3, alcadein-beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232370
Homologene: 22836
Rmdn2
Name: regulator of microtubule dynamics 2
Synonyms: Fam82a1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381110
VEGA: 17
Homologene: 71930
Npr2
Name: natriuretic peptide receptor 2
Synonyms: guanylyl cyclase-B, cn, pwe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230103
HGNC: HGNC:7944
Homologene: 2970
Slc13a2
Name: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2
Synonyms: sodium/dicarboxylate co-transporter, mNaDC-1, Nadc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20500
Homologene: 2965
Ccdc106
Name: coiled-coil domain containing 106
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232821
Homologene: 81845
Smc1b
Name: structural maintenance of chromosomes 1B
Synonyms: SMC1beta, Smc1l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 140557
VEGA: 15
Homologene: 13786
Arhgef4
Name: Rho guanine nucleotide exchange factor (GEF) 4
Synonyms: 9330140K16Rik, Asef, C230030N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226970
HGNC: HGNC:684
Homologene: 49414
Cfap52
Name: cilia and flagella associated protein 52
Synonyms: 4933417B11Rik, 1700019F09Rik, Wdr16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71860
Homologene: 12416
Rad21
Name: RAD21 cohesin complex component
Synonyms: SCC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19357
VEGA: 15
HGNC: HGNC:9811
Homologene: 38161
Akr1c20
Name: aldo-keto reductase family 1, member C20
Synonyms: 2610528B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 116852
Gcnt1
Name: glucosaminyl (N-acetyl) transferase 1, core 2
Synonyms: IGnT, beta-1, 6-N-acetylglucosaminyltransferase, 5630400D21Rik, C2 GlcNAcT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 14537
HGNC: HGNC:4203
Homologene: 37486
Gtf3c4
Name: general transcription factor IIIC, polypeptide 4
Synonyms: KAT12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269252
HGNC: HGNC:4667
Homologene: 8147
Adam7
Name: a disintegrin and metallopeptidase domain 7
Synonyms: EAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 11500
HGNC: HGNC:214
Homologene: 2830
Ddx31
Name: DEAD/H box helicase 31
Synonyms: 5830444G11Rik, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227674
Homologene: 6389
Mpv17l
Name: Mpv17 transgene, kidney disease mutant-like
Synonyms: M-LP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 93734
Homologene: 13547
Lrif1
Name: ligand dependent nuclear receptor interacting factor 1
Synonyms: 2010012G17Rik, 4933421E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 321000
Homologene: 10160
Lrp8
Name: low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
Synonyms: apoER2, 4932703M08Rik, Lr8b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16975
HGNC: HGNC:6700
Homologene: 31250
Tmprss11d
Name: transmembrane protease, serine 11d
Synonyms: AsP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231382
Homologene: 74519
Igkv4-69
Name: immunoglobulin kappa variable 4-69
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 628541
Lrrc23
Name: leucine rich repeat containing 23
Synonyms: 4921537K05Rik, Lrpb7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16977
Homologene: 5082
Or2d2
Name: olfactory receptor family 2 subfamily D member 2
Synonyms: GA_x6K02T2PBJ9-9479517-9478573, MOR260-1, Olfr715
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258776
HGNC: HGNC:8244
Homologene: 81541
Klhl36
Name: kelch-like 36
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234796
Homologene: 32598
Slc18b1
Name: solute carrier family 18, subfamily B, member 1
Synonyms: 1110021L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 76306
Homologene: 33473
Naprt
Name: nicotinate phosphoribosyltransferase
Synonyms: 9130210N20Rik, Naprt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223646
VEGA: 15
Homologene: 82285
Slc16a8
Name: solute carrier family 16 (monocarboxylic acid transporters), member 8
Synonyms: proton-coupled monocarboxylate transporter 3, Mct3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 57274
Homologene: 75006
CT025622.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 4,345,640 bp
  • A to G, chromosome 1 at 34,745,448 bp
  • G to A, chromosome 1 at 86,194,262 bp
  • T to A, chromosome 1 at 194,644,404 bp
  • G to T, chromosome 2 at 28,840,169 bp
  • A to T, chromosome 2 at 28,857,132 bp
  • A to T, chromosome 2 at 31,514,819 bp
  • A to G, chromosome 2 at 32,302,933 bp
  • A to G, chromosome 2 at 66,236,382 bp
  • A to G, chromosome 2 at 126,725,390 bp
  • A to T, chromosome 3 at 106,734,398 bp
  • A to T, chromosome 3 at 116,758,806 bp
  • T to A, chromosome 4 at 43,650,315 bp
  • A to G, chromosome 4 at 107,869,098 bp
  • T to A, chromosome 4 at 108,625,816 bp
  • G to A, chromosome 4 at 154,476,627 bp
  • T to C, chromosome 5 at 4,064,569 bp
  • T to C, chromosome 5 at 73,086,972 bp
  • T to C, chromosome 5 at 86,338,812 bp
  • A to G, chromosome 6 at 36,744,497 bp
  • A to G, chromosome 6 at 69,284,269 bp
  • G to A, chromosome 6 at 124,451,413 bp
  • A to T, chromosome 6 at 124,770,704 bp
  • A to T, chromosome 7 at 5,060,245 bp
  • A to T, chromosome 7 at 28,408,160 bp
  • A to T, chromosome 7 at 56,423,352 bp
  • A to T, chromosome 7 at 107,128,732 bp
  • T to A, chromosome 7 at 141,792,034 bp
  • GGGTGGTG to GGGTG, chromosome 8 at 111,480,540 bp
  • A to G, chromosome 8 at 119,876,551 bp
  • A to T, chromosome 9 at 50,645,119 bp
  • G to A, chromosome 9 at 67,972,861 bp
  • G to A, chromosome 9 at 111,469,163 bp
  • T to A, chromosome 10 at 23,819,078 bp
  • T to C, chromosome 10 at 61,867,483 bp
  • C to T, chromosome 10 at 62,780,457 bp
  • T to C, chromosome 10 at 86,949,928 bp
  • G to T, chromosome 10 at 127,898,953 bp
  • T to C, chromosome 11 at 3,501,150 bp
  • T to C, chromosome 11 at 23,673,331 bp
  • A to G, chromosome 11 at 32,604,190 bp
  • T to A, chromosome 11 at 67,954,088 bp
  • T to C, chromosome 11 at 78,403,175 bp
  • T to A, chromosome 11 at 80,395,507 bp
  • T to A, chromosome 13 at 4,487,208 bp
  • A to C, chromosome 13 at 67,553,025 bp
  • T to G, chromosome 14 at 20,661,398 bp
  • T to A, chromosome 14 at 52,204,826 bp
  • T to C, chromosome 14 at 68,510,856 bp
  • C to T, chromosome 15 at 7,157,191 bp
  • A to T, chromosome 15 at 11,000,817 bp
  • A to T, chromosome 15 at 51,968,358 bp
  • T to A, chromosome 15 at 54,847,237 bp
  • A to G, chromosome 15 at 75,891,069 bp
  • T to A, chromosome 15 at 79,252,607 bp
  • G to A, chromosome 15 at 83,228,493 bp
  • A to G, chromosome 15 at 85,112,673 bp
  • A to G, chromosome 15 at 89,103,065 bp
  • T to C, chromosome 16 at 13,944,719 bp
  • C to A, chromosome 16 at 85,056,414 bp
  • T to C, chromosome 17 at 57,742,126 bp
  • A to G, chromosome 17 at 79,667,955 bp
  • T to C, chromosome 17 at 83,446,058 bp
  • C to T, chromosome 17 at 83,766,587 bp
  • A to T, chromosome 18 at 6,226,980 bp
  • T to C, chromosome 18 at 9,332,917 bp
  • G to A, chromosome 19 at 17,330,316 bp
  • C to T, chromosome 19 at 36,986,653 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0440 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038641-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.