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Strain Name:
C57BL/6J-MtgxR0510Btlr/Mmmh
Stock Number:
038704-MU
Citation ID:
RRID:MMRRC_038704-MU
Other Names:
R0510 (G1), C57BL/6J-MtgxR0510Btlr
Major Collection:

Strain Information

Sptbn4
Name: spectrin beta, non-erythrocytic 4
Synonyms: dyn, SpbIV, neuroaxonal dystrophy, 5830426A08Rik, ROSA62, nmf261, 1700022P15Rik, Spnb4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 80297
Homologene: 11879
Epyc
Name: epiphycan
Synonyms: PG-Lb, epiphycan, SLRR3B, Dspg3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13516
HGNC: HGNC:3053
Homologene: 3634
Cpe
Name: carboxypeptidase E
Synonyms: carboxypeptidase H, CPH, Cph1, Cph-1, NF-alpha1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12876
HGNC: HGNC:2303
Homologene: 48052
Dgat1
Name: diacylglycerol O-acyltransferase 1
Synonyms: D15Ertd23e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13350
HGNC: HGNC:2843
Homologene: 7688
Wdr73
Name: WD repeat domain 73
Synonyms: 1200011I23Rik, 2410008B13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71968
Homologene: 41899
Mtss1
Name: MTSS I-BAR domain containing 1
Synonyms: MIM, D130001D01Rik, 2310003N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 211401
VEGA: 15
Homologene: 8841
Ap2m1
Name: adaptor-related protein complex 2, mu 1 subunit
Synonyms: clathrin-associated AP-2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11773
HGNC: HGNC:564
Homologene: 3000
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 6,249,171 bp
  • T to A, chromosome 1 at 32,545,875 bp
  • T to A, chromosome 1 at 44,061,097 bp
  • T to C, chromosome 1 at 134,409,692 bp
  • T to C, chromosome 1 at 185,260,508 bp
  • T to A, chromosome 1 at 188,734,663 bp
  • T to A, chromosome 1 at 192,418,941 bp
  • T to A, chromosome 1 at 193,178,231 bp
  • GCCACCA to GCCA, chromosome 2 at 52,110,324 bp
  • A to T, chromosome 2 at 52,290,743 bp
  • A to G, chromosome 2 at 76,730,412 bp
  • A to G, chromosome 2 at 87,737,481 bp
  • A to T, chromosome 2 at 89,406,791 bp
  • A to T, chromosome 2 at 121,305,774 bp
  • A to T, chromosome 2 at 125,109,034 bp
  • A to G, chromosome 2 at 125,342,925 bp
  • G to A, chromosome 2 at 127,880,503 bp
  • A to G, chromosome 2 at 145,861,033 bp
  • C to T, chromosome 2 at 170,115,462 bp
  • A to G, chromosome 2 at 174,465,950 bp
  • T to A, chromosome 3 at 9,264,668 bp
  • A to G, chromosome 3 at 88,911,541 bp
  • A to T, chromosome 3 at 114,105,456 bp
  • A to T, chromosome 3 at 144,913,351 bp
  • A to G, chromosome 4 at 132,745,138 bp
  • G to A, chromosome 4 at 135,338,543 bp
  • T to G, chromosome 4 at 139,430,223 bp
  • C to A, chromosome 4 at 151,075,140 bp
  • A to G, chromosome 5 at 104,977,616 bp
  • A to T, chromosome 5 at 113,946,705 bp
  • T to A, chromosome 5 at 121,281,896 bp
  • G to A, chromosome 5 at 121,305,673 bp
  • A to T, chromosome 6 at 30,519,548 bp
  • C to A, chromosome 6 at 50,246,127 bp
  • A to T, chromosome 6 at 85,620,369 bp
  • A to T, chromosome 6 at 101,151,053 bp
  • T to C, chromosome 6 at 146,417,979 bp
  • C to T, chromosome 6 at 149,150,584 bp
  • A to T, chromosome 7 at 19,577,906 bp
  • C to T, chromosome 7 at 27,361,566 bp
  • A to T, chromosome 7 at 41,427,792 bp
  • C to T, chromosome 7 at 45,431,306 bp
  • T to A, chromosome 7 at 45,794,112 bp
  • G to A, chromosome 7 at 64,223,758 bp
  • T to G, chromosome 7 at 75,136,392 bp
  • G to A, chromosome 7 at 80,897,950 bp
  • T to C, chromosome 7 at 86,363,827 bp
  • T to C, chromosome 7 at 97,347,541 bp
  • T to A, chromosome 7 at 99,661,536 bp
  • T to C, chromosome 7 at 121,500,569 bp
  • A to G, chromosome 8 at 12,997,337 bp
  • A to G, chromosome 8 at 21,665,972 bp
  • A to T, chromosome 8 at 22,671,635 bp
  • T to A, chromosome 8 at 45,935,157 bp
  • T to A, chromosome 8 at 64,611,467 bp
  • C to T, chromosome 8 at 69,815,609 bp
  • T to C, chromosome 8 at 72,492,571 bp
  • C to A, chromosome 9 at 15,999,685 bp
  • A to G, chromosome 9 at 55,758,062 bp
  • T to A, chromosome 9 at 70,748,248 bp
  • G to T, chromosome 9 at 76,492,826 bp
  • A to G, chromosome 9 at 106,471,796 bp
  • G to A, chromosome 9 at 108,827,005 bp
  • A to G, chromosome 10 at 5,367,600 bp
  • A to T, chromosome 10 at 24,776,781 bp
  • A to T, chromosome 10 at 74,290,976 bp
  • C to A, chromosome 10 at 75,974,245 bp
  • A to G, chromosome 10 at 97,649,763 bp
  • T to A, chromosome 10 at 117,045,790 bp
  • T to A, chromosome 11 at 29,733,849 bp
  • T to C, chromosome 11 at 69,235,576 bp
  • T to A, chromosome 11 at 99,486,782 bp
  • G to T, chromosome 12 at 3,982,058 bp
  • T to C, chromosome 12 at 75,854,149 bp
  • T to C, chromosome 12 at 101,988,786 bp
  • A to G, chromosome 13 at 18,139,723 bp
  • C to A, chromosome 13 at 29,691,596 bp
  • A to T, chromosome 13 at 67,591,134 bp
  • G to T, chromosome 13 at 111,440,745 bp
  • T to C, chromosome 14 at 37,080,676 bp
  • A to G, chromosome 14 at 41,158,749 bp
  • T to A, chromosome 14 at 50,819,673 bp
  • T to C, chromosome 14 at 54,476,297 bp
  • T to C, chromosome 14 at 55,569,080 bp
  • T to C, chromosome 15 at 33,017,089 bp
  • A to T, chromosome 15 at 58,956,538 bp
  • T to C, chromosome 15 at 76,511,567 bp
  • A to T, chromosome 15 at 76,603,657 bp
  • A to T, chromosome 15 at 88,819,956 bp
  • C to A, chromosome 15 at 101,463,627 bp
  • T to C, chromosome 15 at 101,763,316 bp
  • T to A, chromosome 16 at 20,542,240 bp
  • C to A, chromosome 17 at 19,522,120 bp
  • T to C, chromosome 17 at 29,493,909 bp
  • C to T, chromosome 17 at 34,334,244 bp
  • T to C, chromosome 17 at 57,235,358 bp
  • G to A, chromosome 18 at 32,125,118 bp
  • A to T, chromosome 18 at 34,596,151 bp
  • G to T, chromosome 19 at 6,840,498 bp
  • G to T, chromosome 19 at 8,778,521 bp
  • C to T, chromosome 19 at 9,018,232 bp
  • A to G, chromosome 19 at 56,898,068 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0510 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038704-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.