Strain Name:
C57BL/6J-MtgxR0543Btlr/Mmmh
Stock Number:
038735-MU
Citation ID:
RRID:MMRRC_038735-MU
Other Names:
R0543 (G1), C57BL/6J-MtgxR0543Btlr
Major Collection:

Strain Information

Met
Name: met proto-oncogene
Synonyms: Par4, c-Met, HGF receptor
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Aclp7, trabeculin alpha, Acf7
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, DICE1, Ddx26, 2900075H24Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Trp53bp1
Name: transformation related protein 53 binding protein 1
Synonyms: p53BP1, 53BP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27223
Homologene: 4137
Hells
Name: helicase, lymphoid specific
Synonyms: PASG, YFK8, E130115I21Rik, Lysh, proliferation-associated SNF2-like, LSH
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15201
HGNC: HGNC:4861
Homologene: 50037
Enox1
Name: ecto-NOX disulfide-thiol exchanger 1
Synonyms: D230005D02Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239188
VEGA: 14
Homologene: 56793
Trpm7
Name: transient receptor potential cation channel, subfamily M, member 7
Synonyms: CHAK1, 5033407O22Rik, Ltpr7, 4833414K03Rik, 2310022G15Rik, TRP-PLIK, LTRPC7, CHAK
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58800
Homologene: 9774
Thada
Name: thyroid adenoma associated
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240174
VEGA: 17
Homologene: 75175
Ubr1
Name: ubiquitin protein ligase E3 component n-recognin 1
Synonyms: E3 alpha
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22222
Homologene: 7582
Nup205
Name: nucleoporin 205
Synonyms: 3830404O05Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70699
Homologene: 45971
Rab11fip3
Name: RAB11 family interacting protein 3 (class II)
Synonyms: Rab11-FIP3, D030060O14Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 215445
Homologene: 49396
Bltp1
Name: bridge-like lipid transfer protein family member 1
Synonyms: FSA, 4932438A13Rik, Tweek
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229227
Homologene: 52105
Utp18
Name: UTP18 small subunit processome component
Synonyms: 6230425C22Rik, Wdr50
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217109
Homologene: 41087
Itprid2
Name: ITPR interacting domain containing 2
Synonyms: KRAP, Ssfa2, SPAG13, CS1, CS-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70599
Homologene: 4912
Itpr1
Name: inositol 1,4,5-trisphosphate receptor 1
Synonyms: Itpr-1, wblo, opt, P400, Pcp-1, Pcp1, IP3R1, Ip3r, InsP3R type I
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16438
HGNC: HGNC:6180
Homologene: 1673
Git2
Name: GIT ArfGAP 2
Synonyms: 9630056M03Rik, 1500036H07Rik, 5830420E16Rik, ARF GTPase activating protein 2, Cool associated tyrosine phosphorylated-2, Cat-2, B230104M05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 26431
HGNC: HGNC:4273
Homologene: 41336
Tppp3
Name: tubulin polymerization-promoting protein family member 3
Synonyms: 2700055K07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67971
Homologene: 32659
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 21819
Homologene: 2430
Hnf1a
Name: HNF1 homeobox A
Synonyms: HNF1[a], LFB1, Hnf1alpha, Tcf1, HNF1, HNF1-alpha, hepatocyte nuclear factor 1, Hnf-1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21405
Homologene: 459
Mtch2
Name: mitochondrial carrier 2
Synonyms: 4930539J07Rik, HSPC032, 2310034D24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56428
Homologene: 8645
Nkiras2
Name: NFKB inhibitor interacting Ras-like protein 2
Synonyms: KBRAS2, 4930527H08Rik, 2410003M04Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71966
Homologene: 9738
Tnfrsf21
Name: tumor necrosis factor receptor superfamily, member 21
Synonyms: DR6, Death receptor 6, TR7
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 94185
VEGA: 17
Homologene: 8696
Zfp719
Name: zinc finger protein 719
Synonyms: C630016O21Rik, mszf6, 9430094P17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 210105
Ash1l
Name: ASH1 like histone lysine methyltransferase
Synonyms: chromatin remodeling factor, E430018P19Rik, KMT2H, 8030453L17Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 192195
Homologene: 10225
Ccser2
Name: coiled-coil serine rich 2
Synonyms: Gcap14, 2900054P12Rik, 1700012P13Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 72972
Homologene: 10367
Hoxa5
Name: homeobox A5
Synonyms: Hox-1.3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 15402
HGNC: HGNC:5106
Homologene: 40726
Mcm9
Name: minichromosome maintenance 9 homologous recombination repair factor
Synonyms: Mcmdc1, 9030408O17Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71567
VEGA: 10
Homologene: 13546
Mcf2l
Name: mcf.2 transforming sequence-like
Synonyms: C130040G20Rik, Dbs
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17207
Homologene: 11804
Gm7964
Name: predicted gene 7964
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 666170
Fgfr3
Name: fibroblast growth factor receptor 3
Synonyms: Fgfr-3, HBGFR, sam3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14184
HGNC: HGNC:3690
Homologene: 55437
Ecrg4
Name: ECRG4 augurin precursor
Synonyms: augurin, 1500015O10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 78896
Homologene: 11505
Fuca2
Name: fucosidase, alpha-L- 2, plasma
Synonyms: 5530401P20Rik, 0610025O11Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66848
HGNC: HGNC:4008
Homologene: 119
Zdhhc5
Name: zinc finger, DHHC domain containing 5
Synonyms: Zisp, 1110032A17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228136
Homologene: 9147
Inpp4a
Name: inositol polyphosphate-4-phosphatase, type I
Synonyms: 107kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269180
HGNC: HGNC:6074
Homologene: 2871
Dock2
Name: dedicator of cyto-kinesis 2
Synonyms: CED-5, MBC, Hch
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 94176
HGNC: HGNC:2988
Homologene: 37984
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Psd
Name: pleckstrin and Sec7 domain containing
Synonyms: Psdl, Efa6a, Efa6, 1110007H17Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 73728
VEGA: 19
HGNC: HGNC:9507
Homologene: 31115
Cntn3
Name: contactin 3
Synonyms: Pang
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18488
HGNC: HGNC:2173
Homologene: 7461
Mttp
Name: microsomal triglyceride transfer protein
Synonyms: 1810043K16Rik, MTP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17777
HGNC: HGNC:7467
Homologene: 212
Col28a1
Name: collagen, type XXVIII, alpha 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 213945
Homologene: 66345
Pcdhac1
Name: protocadherin alpha subfamily C, 1
Synonyms: CNRc1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 353236
HGNC: HGNC:8676
Homologene: 49561
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, fibrocystin L, PKHDL1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 192190
Homologene: 16332
Slc38a4
Name: solute carrier family 38, member 4
Synonyms: SNAT4, Ata3, 1700012A18Rik, 1110012E16Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69354
VEGA: 15
Homologene: 75077
Muc5b
Name: mucin 5, subtype B, tracheobronchial
Synonyms: 2300002I04Rik, MUC5, MUC9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74180
HGNC: HGNC:7516
Homologene: 136756
Ccdc180
Name: coiled-coil domain containing 180
Synonyms: E230008N13Rik, LOC381522
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381522
Homologene: 117988
Stxbp5l
Name: syntaxin binding protein 5-like
Synonyms: insulin level locus 1, tomosyn-2, A830015P08Rik, LLGL4, T2dm1, t2md1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 207227
Homologene: 18173
Pik3ca
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Synonyms: p110alpha, 6330412C24Rik, caPI3K
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18706
HGNC: HGNC:8975
Homologene: 21249
Tns1
Name: tensin 1
Synonyms: E030018G17Rik, E030037J05Rik, 1110018I21Rik, 1200014E20Rik, Tns
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21961
Homologene: 11219
Slco6c1
Name: solute carrier organic anion transporter family, member 6c1
Synonyms: 4933404A18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74441
Homologene: 65259
Rpl22l1
Name: ribosomal protein L22 like 1
Synonyms: 3110001N18Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68028
Homologene: 134718
Dsg1a
Name: desmoglein 1 alpha
Synonyms: Dsg1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13510
HGNC: HGNC:3048
Homologene: 1463
Cdcp2
Name: CUB domain containing protein 2
Synonyms: D030010E02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242603
Homologene: 138406
Mettl14
Name: methyltransferase 14, N6-adenosine-methyltransferase subunit
Synonyms: G430022H21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 210529
Homologene: 10865
Hars2
Name: histidyl-tRNA synthetase 2
Synonyms: Harsl, HARSR, HO3, 4631412B19Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 70791
HGNC: HGNC:4817
Homologene: 40819
Strip1
Name: striatin interacting protein 1
Synonyms: Fam40a, 6330569M22Rik, 6530401O14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229707
Homologene: 35064
Kcnt2
Name: potassium channel, subfamily T, member 2
Synonyms: E330038N15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240776
Homologene: 16121
Clca3a1
Name: chloride channel accessory 3A1
Synonyms: Clca1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12722
HGNC: HGNC:2017
Homologene: 77224
Alox5
Name: arachidonate 5-lipoxygenase
Synonyms: 5LO, 5-LOX, 5LX
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11689
HGNC: HGNC:435
Homologene: 561
Nostrin
Name: nitric oxide synthase trafficker
Synonyms: mDaIP2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329416
Homologene: 43142
Or5b21
Name: olfactory receptor family 5 subfamily B member 21
Synonyms: MOR202-4, Olfr1444, GA_x6K02T2RE5P-3191201-3192160
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258697
Homologene: 64906
Prkn
Name: parkin RBR E3 ubiquitin protein ligase
Synonyms: PRKN, Park2, Parkin
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 50873
HGNC: HGNC:8607
Homologene: 3355
Mrgpra4
Name: MAS-related GPR, member A4
Synonyms: MrgA4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 235854
Homologene: 131159
Oxct2b
Name: 3-oxoacid CoA transferase 2B
Synonyms: Scot-t2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 353371
Homologene: 137359
Lyg2
Name: lysozyme G-like 2
Synonyms: LOC332427
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 332427
Homologene: 18483
Or5w17
Name: olfactory receptor family 5 subfamily W member 17
Synonyms: MOR177-10, Olfr1141, GA_x6K02T2Q125-49257818-49256883
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258630
Homologene: 74080
Or12j3
Name: olfactory receptor family 12 subfamily J member 3
Synonyms: Olfr530, GA_x6K02T2PBJ9-42523824-42522901, MOR252-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258512
Homologene: 128384
Nherf4
Name: NHERF family PDZ scaffold protein 4
Synonyms: Pdzd3, sodium-phosphate cotransporter IIa C-terminal-associated protein 2, Pdzk2, NaPi-Cap2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 170761
VEGA: 9
Homologene: 11719
Plscr1
Name: phospholipid scramblase 1
Synonyms: NOR1, MuPLSCR2, MmTRA1b, MmTRA1a, TRA1, Tras2, Tras1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22038
HGNC: HGNC:9092
Homologene: 41418
Semp2l2b
Name: SUMO/sentrin specific peptidase 2-like 2B
Synonyms: 4930444G20Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 114671
Homologene: 130042
Apol9a
Name: apolipoprotein L 9a
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223672
VEGA: 15
Homologene: 77567
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 37,369,492 bp
  • T to A, chromosome 1 at 37,911,107 bp
  • C to A, chromosome 1 at 43,742,289 bp
  • T to A, chromosome 1 at 73,952,697 bp
  • T to A, chromosome 1 at 97,127,898 bp
  • C to A, chromosome 1 at 140,609,614 bp
  • T to G, chromosome 2 at 69,189,131 bp
  • T to A, chromosome 2 at 79,644,506 bp
  • T to A, chromosome 2 at 84,692,480 bp
  • C to A, chromosome 2 at 87,753,650 bp
  • T to C, chromosome 2 at 90,849,682 bp
  • A to G, chromosome 2 at 120,881,093 bp
  • C to A, chromosome 2 at 121,251,868 bp
  • T to C, chromosome 2 at 126,848,529 bp
  • C to A, chromosome 3 at 28,807,274 bp
  • G to A, chromosome 3 at 32,450,261 bp
  • G to A, chromosome 3 at 36,996,458 bp
  • T to A, chromosome 3 at 89,063,778 bp
  • G to A, chromosome 3 at 107,626,775 bp
  • A to T, chromosome 3 at 123,374,762 bp
  • A to T, chromosome 3 at 138,111,696 bp
  • T to C, chromosome 3 at 144,748,394 bp
  • A to T, chromosome 4 at 45,900,041 bp
  • A to T, chromosome 4 at 107,097,676 bp
  • T to A, chromosome 4 at 123,116,989 bp
  • G to T, chromosome 4 at 123,376,378 bp
  • A to G, chromosome 5 at 33,729,710 bp
  • G to T, chromosome 5 at 114,745,531 bp
  • G to A, chromosome 5 at 114,950,744 bp
  • T to A, chromosome 6 at 8,075,326 bp
  • T to A, chromosome 6 at 17,491,970 bp
  • T to C, chromosome 6 at 35,198,969 bp
  • T to C, chromosome 6 at 52,204,340 bp
  • G to A, chromosome 6 at 102,269,090 bp
  • T to C, chromosome 6 at 108,515,748 bp
  • T to A, chromosome 6 at 116,454,317 bp
  • A to G, chromosome 7 at 43,589,253 bp
  • T to C, chromosome 7 at 47,981,310 bp
  • G to A, chromosome 7 at 83,756,394 bp
  • A to G, chromosome 7 at 140,373,394 bp
  • A to G, chromosome 7 at 141,851,785 bp
  • T to C, chromosome 8 at 12,996,728 bp
  • T to C, chromosome 8 at 105,468,208 bp
  • A to C, chromosome 9 at 44,248,934 bp
  • A to T, chromosome 9 at 92,258,046 bp
  • T to A, chromosome 10 at 13,503,126 bp
  • T to C, chromosome 10 at 22,066,924 bp
  • C to T, chromosome 10 at 53,541,598 bp
  • A to G, chromosome 11 at 34,294,325 bp
  • A to T, chromosome 11 at 60,479,051 bp
  • A to T, chromosome 11 at 93,875,835 bp
  • G to A, chromosome 11 at 100,624,192 bp
  • A to T, chromosome 14 at 36,940,192 bp
  • T to C, chromosome 14 at 62,696,611 bp
  • T to C, chromosome 14 at 77,506,959 bp
  • A to G, chromosome 15 at 44,523,491 bp
  • A to T, chromosome 15 at 66,729,597 bp
  • A to G, chromosome 15 at 77,735,640 bp
  • A to T, chromosome 15 at 97,016,839 bp
  • T to A, chromosome 16 at 32,756,746 bp
  • G to A, chromosome 16 at 37,208,096 bp
  • G to A, chromosome 17 at 11,067,179 bp
  • T to C, chromosome 17 at 25,994,225 bp
  • C to T, chromosome 17 at 43,038,213 bp
  • T to C, chromosome 17 at 84,423,163 bp
  • A to T, chromosome 18 at 20,340,863 bp
  • G to A, chromosome 18 at 36,789,424 bp
  • A to T, chromosome 18 at 37,185,068 bp
  • G to T, chromosome 19 at 12,861,888 bp
  • A to G, chromosome 19 at 38,967,750 bp
  • T to C, chromosome 19 at 46,319,517 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0543 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038735-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.