Strain Name:
C57BL/6J-MtgxR0613Btlr/Mmmh
Stock Number:
038802-MU
Citation ID:
RRID:MMRRC_038802-MU
Other Names:
R0613 (G1), C57BL/6J-MtgxR0613Btlr
Major Collection:

Gene Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: TonEBP, nfatz, B130038B15Rik, OREBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Adcy9
Name: adenylate cyclase 9
Synonyms: D16Wsu65e, ACtp10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11515
HGNC: HGNC:240
Homologene: 868
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20269
Homologene: 56005
Xpnpep1
Name: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Synonyms: D230045I08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 170750
Homologene: 6424
Map1b
Name: microtubule-associated protein 1B
Synonyms: MAP5, LC1, Mtap-5, Mtap5, Mtap1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17755
VEGA: 13
HGNC: HGNC:6836
Homologene: 38111
Sdhc
Name: succinate dehydrogenase complex, subunit C, integral membrane protein
Synonyms: 0610010E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66052
Homologene: 2256
Mtor
Name: mechanistic target of rapamycin kinase
Synonyms: Frap1, RAPT1, RAFT1, FKBP-rapamycin-associated protein FRAP, 2610315D21Rik, flat, mechanistic target of rapamycin (serine/threonine kinase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56717
HGNC: HGNC:3942
Homologene: 3637
Ahctf1
Name: AT hook containing transcription factor 1
Synonyms: Elys, 6230412P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226747
Homologene: 9142
Gtf3c1
Name: general transcription factor III C 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233863
HGNC: HGNC:4664
Homologene: 31040
Cep170
Name: centrosomal protein 170
Synonyms: A330004A13Rik, 4933426L22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545389
Homologene: 22844
Brca1
Name: breast cancer 1, early onset
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12189
HGNC: HGNC:1100
Homologene: 5276
Prpf8
Name: pre-mRNA processing factor 8
Synonyms: Sfprp8l, Prp8, D11Bwg0410e, DBF3/PRP8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192159
Homologene: 4706
Tmem161b
Name: transmembrane protein 161B
Synonyms: 2810446P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72745
Homologene: 14519
Prelid1
Name: PRELI domain containing 1
Synonyms: 2610524G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66494
VEGA: 13
Homologene: 40859
Gucy2c
Name: guanylate cyclase 2c
Synonyms: GC-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14917
HGNC: HGNC:4688
Homologene: 3641
Adgrl4
Name: adhesion G protein-coupled receptor L4
Synonyms: Etl, 1110033N21Rik, EGF-TM7 receptor, Eltd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170757
Homologene: 11170
Hspa9
Name: heat shock protein 9
Synonyms: mortalin, Hsp74, PBP74, GRP75, mthsp70, mot-2, Hsp74a, Hspa9a, CSA, C3H-specific antigen, Hsc74
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 15526
HGNC: HGNC:5244
Homologene: 39452
Rab4a
Name: RAB4A, member RAS oncogene family
Synonyms: Rab4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 19341
HGNC: HGNC:9781
Homologene: 101538
Nelfa
Name: negative elongation factor complex member A, Whsc2
Synonyms: Whsc2, Whsc2h, Nelf-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 24116
Homologene: 68478
Gpr107
Name: G protein-coupled receptor 107
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 277463
Homologene: 81077
Ppip5k2
Name: diphosphoinositol pentakisphosphate kinase 2
Synonyms: Vip2, Hisppd1, Cfap160
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227399
Homologene: 49409
Emc1
Name: ER membrane protein complex subunit 1
Synonyms: C230096C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230866
Homologene: 9002
Atp12a
Name: ATPase, H+/K+ transporting, nongastric, alpha polypeptide
Synonyms: cHKA, HKalpha2, Atp1al1, ATPase H+K+-transporting, alpha 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 192113
VEGA: 14
Homologene: 68197
Hps6
Name: HPS6, biogenesis of lysosomal organelles complex 2 subunit 3
Synonyms: 5330434M19Rik, Hermansky-Pudlak syndrome 6, BLOC-2, ru, ruby eye
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20170
VEGA: 19
Homologene: 11691
Aff3
Name: AF4/FMR2 family, member 3
Synonyms: 3222402O04Rik, LAF-4, Laf4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16764
HGNC: HGNC:6473
Homologene: 1718
Mrc1
Name: mannose receptor, C type 1
Synonyms: MR, CD206
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17533
HGNC: HGNC:7228
Homologene: 37622
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241516
Homologene: 110349
Itgb4
Name: integrin beta 4
Synonyms: CD104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192897
HGNC: HGNC:6158
Homologene: 179
D430041D05Rik
Name: RIKEN cDNA D430041D05 gene
Synonyms: G2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241589
Homologene: 115928
Tmem132e
Name: transmembrane protein 132E
Synonyms: LOC270893
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 270893
Homologene: 41524
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Kcnh4
Name: potassium voltage-gated channel, subfamily H (eag-related), member 4
Synonyms: BEC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380728
HGNC: HGNC:6253
Homologene: 8180
Syt11
Name: synaptotagmin XI
Synonyms: 6530420C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229521
Homologene: 23120
Mfsd6
Name: major facilitator superfamily domain containing 6
Synonyms: MMR2, 2210010L05Rik, 9630025I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98682
Homologene: 9784
Khdrbs2
Name: KH domain containing, RNA binding, signal transduction associated 2
Synonyms: 6330586C16Rik, SLM-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 170771
Homologene: 15772
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: bl, E130113P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231470
Homologene: 23516
Slco3a1
Name: solute carrier organic anion transporter family, member 3a1
Synonyms: MJAM, Slc21a11, OATP-D, 5830414C08Rik, Anr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 108116
Homologene: 40862
Otogl
Name: otogelin-like
Synonyms: Gm6924
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 628870
Homologene: 46008
Grik1
Name: glutamate receptor, ionotropic, kainate 1
Synonyms: A830007B11Rik, Glur-5, D16Ium24e, GluK5, Glur5, Glurbeta1, D16Ium24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 14805
HGNC: HGNC:4579
Homologene: 68992
Vmn2r105
Name: vomeronasal 2, receptor 105
Synonyms: EG627743
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627743
Homologene: 129680
Nepn
Name: nephrocan
Synonyms: Npn, periolin, 5730521E12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66650
Homologene: 23549
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: AI414108, LOC235086
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235086
Homologene: 19472
Zfp518b
Name: zinc finger protein 518B
Synonyms: 6820424L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100515
Homologene: 19115
Tll2
Name: tolloid-like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 24087
VEGA: 19
Homologene: 56545
Gpr108
Name: G protein-coupled receptor 108
Synonyms: 1810015L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78308
VEGA: 17
Homologene: 32495
Vstm2a
Name: V-set and transmembrane domain containing 2A
Synonyms: Vstm2, 9330184N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 211739
Homologene: 17123
Nipal4
Name: NIPA-like domain containing 4
Synonyms: 9530066K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 214112
Homologene: 133769
Cyp2j12
Name: cytochrome P450, family 2, subfamily j, polypeptide 12
Synonyms: Cyp2j12-ps, OTTMUSG00000007939
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242546
Homologene: 133195
Lrrc31
Name: leucine rich repeat containing 31
Synonyms: E230002P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 320352
Fam189a2
Name: family with sequence similarity 189, member A2
Synonyms: LOC381217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 381217
VEGA: 19
Homologene: 3540
Ptprb
Name: protein tyrosine phosphatase, receptor type, B
Synonyms: VE-PTP, 3230402H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19263
VEGA: 10
HGNC: HGNC:9665
Homologene: 2125
Cntnap3
Name: contactin associated protein-like 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238680
VEGA: 13
Homologene: 129602
Mgst1
Name: microsomal glutathione S-transferase 1
Synonyms: 1500002K10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56615
HGNC: HGNC:7061
Homologene: 10544
Rab3il1
Name: RAB3A interacting protein (rabin3)-like 1
Synonyms: Rab3ail1, 1200014K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74760
HGNC: HGNC:9780
Homologene: 40880
Olfr747
Name: olfactory receptor 747
Synonyms: GA_x6K02T2PMLR-6420220-6419279, MOR106-7, MOR106-16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258264
Homologene: 10652
Olfr1228
Name: olfactory receptor 1228
Synonyms: MOR233-1, GA_x6K02T2Q125-50696209-50695274
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258973
Homologene: 74217
Igsf8
Name: immunoglobulin superfamily, member 8
Synonyms: EWI-2, PG regulatory-like protein, ESTM34, PGRL, KCT-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 140559
Homologene: 14163
Syne3
Name: spectrin repeat containing, nuclear envelope family member 3
Synonyms: nesprin-3beta, 4831426I19Rik, nesprin-3alpha, nesprin-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 212073
VEGA: 12
Homologene: 17625
Olfr1391
Name: olfactory receptor 1391
Synonyms: GA_x6K02T2QP88-6107233-6106298, MOR256-27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258460
Homologene: 17264
Zfp69
Name: zinc finger protein 69
Synonyms: KRAB2, Zfp63, LOC381549
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381549
Homologene: 45693
Ces1a
Name: carboxylesterase 1A
Synonyms: Gm4976
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244595
HGNC: HGNC:1863
Homologene: 134142
Olfr809
Name: olfactory receptor 809
Synonyms: GA_x6K02T2PULF-11454600-11455541, MOR108-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258321
Homologene: 138308
Zfp112
Name: zinc finger protein 112
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 57745
Homologene: 49338
Ccl25
Name: chemokine (C-C motif) ligand 25
Synonyms: Scya25, TECK, CKb15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20300
Homologene: 4109
Kmo
Name: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98256
HGNC: HGNC:6381
Homologene: 2729
Edn2
Name: endothelin 2
Synonyms: VIC, vasoactive intestinal contractor peptide
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13615
HGNC: HGNC:3177
Homologene: 1477
Zfp865
Name: zinc finger protein 865
Synonyms: 6430526N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 319748
Homologene: 19652
Acd
Name: adrenocortical dysplasia
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 497652
Homologene: 23391
Olfr924
Name: olfactory receptor 924
Synonyms: MOR171-27P, MOR171-47, Olfr1520-ps1, GA_x6K02T2PVTD-32543982-32544908, MOR171-27P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 404322
VEGA: 9
HGNC: HGNC:8482
Homologene: 128215
Olfr462
Name: olfactory receptor 462
Synonyms: MOR240-3, GA_x6K02T2PAEV-9536824-9535889
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258406
HGNC: HGNC:8294
Homologene: 64870
Ctsm
Name: cathepsin M
Synonyms: 1600027J17Rik, Catm, Cat M
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 64139
VEGA: 13
Homologene: 75181
Ncoa4
Name: nuclear receptor coactivator 4
Synonyms: 4432406M01Rik, 1110034E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 27057
HGNC: HGNC:7671
Homologene: 38052
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 32,657,522 bp
  • T to C, chromosome 1 at 38,209,923 bp
  • T to C, chromosome 1 at 52,658,696 bp
  • G to A, chromosome 1 at 88,243,950 bp
  • A to C, chromosome 1 at 97,752,740 bp
  • A to T, chromosome 1 at 171,129,844 bp
  • T to A, chromosome 1 at 172,317,589 bp
  • C to A, chromosome 1 at 175,637,892 bp
  • T to C, chromosome 1 at 176,774,680 bp
  • A to G, chromosome 1 at 179,769,414 bp
  • C to T, chromosome 2 at 14,294,819 bp
  • A to G, chromosome 2 at 31,178,285 bp
  • T to A, chromosome 2 at 65,472,284 bp
  • A to T, chromosome 2 at 82,993,795 bp
  • A to T, chromosome 2 at 89,249,125 bp
  • G to C, chromosome 2 at 104,167,950 bp
  • A to G, chromosome 3 at 30,685,035 bp
  • A to G, chromosome 3 at 88,762,469 bp
  • T to C, chromosome 3 at 151,543,222 bp
  • T to G, chromosome 4 at 96,102,079 bp
  • T to A, chromosome 4 at 120,161,864 bp
  • T to C, chromosome 4 at 120,934,347 bp
  • T to A, chromosome 4 at 139,375,072 bp
  • T to C, chromosome 4 at 148,526,046 bp
  • G to A, chromosome 5 at 33,903,463 bp
  • A to T, chromosome 5 at 38,673,603 bp
  • T to C, chromosome 5 at 96,700,488 bp
  • A to T, chromosome 6 at 136,760,723 bp
  • G to A, chromosome 6 at 138,156,245 bp
  • A to G, chromosome 7 at 5,029,091 bp
  • G to A, chromosome 7 at 24,127,028 bp
  • T to C, chromosome 7 at 74,346,634 bp
  • G to A, chromosome 7 at 125,644,134 bp
  • T to C, chromosome 8 at 4,349,850 bp
  • A to G, chromosome 8 at 93,025,581 bp
  • A to T, chromosome 8 at 105,700,568 bp
  • A to G, chromosome 8 at 107,366,295 bp
  • T to C, chromosome 8 at 123,823,835 bp
  • T to C, chromosome 9 at 27,326,920 bp
  • T to A, chromosome 9 at 38,848,613 bp
  • T to A, chromosome 10 at 52,401,257 bp
  • A to T, chromosome 10 at 107,817,070 bp
  • A to T, chromosome 10 at 116,302,325 bp
  • A to G, chromosome 10 at 116,302,378 bp
  • T to A, chromosome 10 at 129,776,262 bp
  • A to T, chromosome 11 at 16,263,140 bp
  • A to T, chromosome 11 at 46,150,384 bp
  • T to A, chromosome 11 at 49,327,748 bp
  • T to C, chromosome 11 at 75,503,444 bp
  • T to A, chromosome 11 at 82,438,338 bp
  • A to T, chromosome 11 at 87,889,227 bp
  • C to T, chromosome 11 at 100,746,932 bp
  • A to T, chromosome 11 at 101,508,210 bp
  • A to T, chromosome 11 at 115,993,342 bp
  • T to C, chromosome 12 at 104,958,112 bp
  • C to T, chromosome 13 at 55,324,343 bp
  • T to C, chromosome 13 at 61,539,682 bp
  • A to T, chromosome 13 at 64,758,414 bp
  • C to T, chromosome 13 at 84,251,320 bp
  • T to A, chromosome 13 at 99,441,641 bp
  • T to A, chromosome 14 at 32,176,552 bp
  • T to A, chromosome 14 at 50,681,404 bp
  • T to A, chromosome 14 at 56,374,521 bp
  • A to G, chromosome 16 at 4,419,539 bp
  • A to G, chromosome 16 at 88,051,333 bp
  • T to A, chromosome 17 at 20,208,316 bp
  • A to G, chromosome 17 at 57,238,174 bp
  • A to T, chromosome 18 at 34,947,980 bp
  • T to C, chromosome 19 at 10,028,364 bp
  • T to A, chromosome 19 at 23,986,489 bp
  • G to T, chromosome 19 at 41,104,990 bp
  • C to A, chromosome 19 at 46,003,821 bp
  • G to T, chromosome 19 at 53,006,353 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0613 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038802-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.