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Strain Name:
C57BL/6J-MtgxR0625Btlr/Mmmh
Stock Number:
038814-MU
Citation ID:
RRID:MMRRC_038814-MU
Other Names:
R0625 (G1), C57BL/6J-MtgxR0625Btlr
Major Collection:

Strain Information

Nes
Name: nestin
Synonyms: RC2, Marc2, ESTM46, Ifaprc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Foxm1
Name: forkhead box M1
Synonyms: Mpm2, WIN, HFH-11B, Trident, Fkh16, Foxm1b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14235
HGNC: HGNC:3818
Homologene: 7318
Usp7
Name: ubiquitin specific peptidase 7
Synonyms: 2210010O09Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 252870
Homologene: 2592
Cxxc5
Name: CXXC finger 5
Synonyms: 4930415K17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67393
VEGA: 18
Homologene: 9517
Qtrt1
Name: queuine tRNA-ribosyltransferase catalytic subunit 1
Synonyms: Tgt, tRNA-guanine transglycosylase, 2610028E17Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 60507
VEGA: 9
Homologene: 11061
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 16,369,743 bp
  • C to T, chromosome 2 at 10,123,414 bp
  • T to A, chromosome 2 at 87,021,620 bp
  • T to A, chromosome 2 at 87,491,008 bp
  • T to C, chromosome 2 at 113,841,711 bp
  • T to A, chromosome 2 at 120,305,041 bp
  • T to C, chromosome 2 at 120,713,990 bp
  • C to T, chromosome 2 at 131,040,256 bp
  • G to A, chromosome 3 at 59,247,437 bp
  • T to G, chromosome 3 at 66,981,544 bp
  • A to G, chromosome 3 at 85,730,500 bp
  • A to G, chromosome 3 at 87,977,172 bp
  • A to G, chromosome 4 at 45,284,055 bp
  • A to G, chromosome 4 at 86,887,162 bp
  • T to C, chromosome 4 at 88,435,010 bp
  • T to A, chromosome 4 at 112,624,086 bp
  • C to G, chromosome 4 at 128,723,710 bp
  • T to C, chromosome 4 at 141,841,518 bp
  • C to T, chromosome 4 at 155,659,460 bp
  • G to A, chromosome 5 at 27,765,942 bp
  • T to A, chromosome 5 at 36,024,572 bp
  • A to T, chromosome 5 at 110,325,550 bp
  • T to C, chromosome 5 at 120,899,259 bp
  • T to C, chromosome 5 at 129,171,931 bp
  • T to A, chromosome 5 at 150,576,745 bp
  • G to A, chromosome 6 at 84,111,987 bp
  • G to A, chromosome 6 at 88,849,955 bp
  • A to G, chromosome 6 at 113,408,903 bp
  • A to G, chromosome 6 at 128,373,871 bp
  • T to A, chromosome 6 at 146,166,651 bp
  • T to C, chromosome 7 at 107,991,189 bp
  • A to G, chromosome 7 at 120,071,887 bp
  • A to C, chromosome 7 at 120,435,893 bp
  • T to C, chromosome 7 at 141,846,427 bp
  • A to T, chromosome 8 at 27,045,131 bp
  • C to T, chromosome 8 at 56,545,395 bp
  • A to G, chromosome 8 at 80,720,686 bp
  • G to T, chromosome 9 at 21,418,288 bp
  • T to C, chromosome 9 at 38,473,208 bp
  • T to C, chromosome 9 at 53,408,065 bp
  • T to C, chromosome 9 at 54,382,702 bp
  • T to A, chromosome 9 at 67,759,751 bp
  • T to G, chromosome 10 at 52,312,850 bp
  • A to T, chromosome 11 at 49,683,171 bp
  • T to A, chromosome 11 at 51,729,454 bp
  • T to C, chromosome 11 at 101,087,782 bp
  • T to A, chromosome 12 at 100,355,046 bp
  • A to T, chromosome 13 at 27,710,140 bp
  • A to G, chromosome 14 at 33,366,714 bp
  • T to C, chromosome 14 at 56,372,837 bp
  • A to T, chromosome 14 at 67,841,163 bp
  • T to C, chromosome 14 at 70,225,027 bp
  • A to G, chromosome 15 at 26,311,043 bp
  • A to G, chromosome 15 at 34,471,369 bp
  • T to C, chromosome 16 at 8,412,102 bp
  • C to T, chromosome 16 at 8,704,982 bp
  • C to T, chromosome 16 at 13,640,294 bp
  • T to G, chromosome 17 at 32,634,678 bp
  • T to C, chromosome 18 at 9,917,195 bp
  • T to G, chromosome 18 at 35,858,589 bp
  • A to G, chromosome 18 at 56,811,830 bp
  • G to A, chromosome 19 at 29,007,705 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0625 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038814-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.