Strain Name:
C57BL/6J-MtgxR0665Btlr/Mmmh
Stock Number:
038850-MU
Citation ID:
RRID:MMRRC_038850-MU
Other Names:
R0665 (G1), C57BL/6J-MtgxR0665Btlr
Major Collection:

Gene Information

Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20586
Homologene: 135927
Dap3
Name: death associated protein 3
Synonyms: 4921514D13Rik, DAP-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 65111
HGNC: HGNC:2673
Homologene: 3404
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20269
Homologene: 56005
Sdhc
Name: succinate dehydrogenase complex, subunit C, integral membrane protein
Synonyms: 0610010E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66052
Homologene: 2256
Lcmt1
Name: leucine carboxyl methyltransferase 1
Synonyms: Lcmt, LCMT-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 30949
Homologene: 41123
Kansl1
Name: KAT8 regulatory NSL complex subunit 1
Synonyms: 1700081L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76719
Homologene: 9140
Triobp
Name: TRIO and F-actin binding protein
Synonyms: Mus EST 478828, Tara, EST478828
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110253
Homologene: 5104
Ttc5
Name: tetratricopeptide repeat domain 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219022
VEGA: 14
Homologene: 14397
Ppp2r5d
Name: protein phosphatase 2, regulatory subunit B', delta
Synonyms: TEG-271, Tex271, B'delta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21770
VEGA: 17
HGNC: HGNC:9312
Homologene: 37661
Kri1
Name: KRI1 homolog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 215194
Homologene: 133742
B3galt2
Name: UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26878
HGNC: HGNC:917
Homologene: 74512
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, P1-Loop, Dnahc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Gtpbp1
Name: GTP binding protein 1
Synonyms: GP-1, GTPBP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 14904
VEGA: 15
HGNC: HGNC:4669
Homologene: 3165
Slc16a4
Name: solute carrier family 16 (monocarboxylic acid transporters), member 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229699
Homologene: 74529
Trpc6
Name: transient receptor potential cation channel, subfamily C, member 6
Synonyms: mtrp6, Trrp6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22068
VEGA: 9
Homologene: 37944
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, LOC382129, D430038H04Rik, 9430076A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Ralgds
Name: ral guanine nucleotide dissociation stimulator
Synonyms: RalGDS, Gnds, Rgds
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19730
HGNC: HGNC:9842
Homologene: 4562
Cps1
Name: carbamoyl-phosphate synthetase 1
Synonyms: CPS, CPSase I, 4732433M03Rik, D1Ucla3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227231
HGNC: HGNC:2323
Homologene: 68208
Espnl
Name: espin-like
Synonyms: LOC227357
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227357
Homologene: 77795
Flnc
Name: filamin C, gamma
Synonyms: Fln2, 1110055E19Rik, actin binding protein 280
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68794
HGNC: HGNC:3756
Homologene: 37481
Tgfbr3
Name: transforming growth factor, beta receptor III
Synonyms: betaglycan, TBRIII, 1110036H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21814
Homologene: 2436
Or5w13
Name: olfactory receptor family 5 subfamily W member 13
Synonyms: GA_x6K02T2Q125-49193051-49192119, MOR177-3, Olfr1136
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258653
Homologene: 74193
Agbl2
Name: ATP/GTP binding protein-like 2
Synonyms: A430081C19Rik, Ccp2, Ccp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 271813
Homologene: 11715
Phyhd1
Name: phytanoyl-CoA dioxygenase domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227696
Homologene: 14939
Myom3
Name: myomesin family, member 3
Synonyms: 8430427K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242702
Homologene: 19432
Or1j4
Name: olfactory receptor family 1 subfamily J member 4
Synonyms: GA_x6K02T2NLDC-33544602-33545540, MOR136-13, Olfr350
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258620
HGNC: HGNC:8211
Homologene: 64897
Chml
Name: choroideremia-like
Synonyms: Rep2, E030003F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12663
HGNC: HGNC:1941
Homologene: 31055
Glb1l
Name: galactosidase, beta 1-like
Synonyms: 4833408P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74577
Homologene: 75208
Dppa3
Name: developmental pluripotency-associated 3
Synonyms: PGC7, 2410075G02Rik, stella
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 73708
Homologene: 138189
Kcnk10
Name: potassium channel, subfamily K, member 10
Synonyms: 3010005K24Rik, 1700024D23Rik, Trek2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 72258
VEGA: 12
HGNC: HGNC:6273
Homologene: 11321
Calr4
Name: calreticulin 4
Synonyms: 4933403L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 108802
Homologene: 86813
Gtf2h2
Name: general transcription factor II H, polypeptide 2
Synonyms: Btf2p44, basal transcription factor 2, p44 subunit, 44kDa, p44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 23894
Homologene: 1159
Antxrl
Name: anthrax toxin receptor-like
Synonyms: 1700112N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239029
VEGA: 14
Homologene: 52111
Or12e1
Name: olfactory receptor family 12 subfamily E member 1
Synonyms: GA_x6K02T2Q125-48676316-48677272, MOR264-6, Olfr1112
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258655
Homologene: 64901
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 67,172,879 bp
  • C to T, chromosome 1 at 75,202,394 bp
  • T to G, chromosome 1 at 91,334,687 bp
  • T to C, chromosome 1 at 143,646,453 bp
  • T to C, chromosome 1 at 171,136,057 bp
  • T to A, chromosome 1 at 175,687,895 bp
  • A to G, chromosome 2 at 28,545,206 bp
  • A to G, chromosome 2 at 30,281,028 bp
  • T to C, chromosome 2 at 36,850,190 bp
  • C to A, chromosome 2 at 65,484,411 bp
  • A to G, chromosome 2 at 87,192,308 bp
  • C to T, chromosome 2 at 87,693,808 bp
  • A to G, chromosome 2 at 90,801,210 bp
  • A to T, chromosome 3 at 88,930,997 bp
  • A to C, chromosome 3 at 107,297,939 bp
  • A to T, chromosome 4 at 109,242,264 bp
  • A to G, chromosome 4 at 135,764,926 bp
  • A to T, chromosome 5 at 107,177,850 bp
  • G to A, chromosome 6 at 29,455,531 bp
  • A to G, chromosome 6 at 122,629,980 bp
  • G to A, chromosome 7 at 123,402,871 bp
  • A to C, chromosome 9 at 8,634,122 bp
  • C to T, chromosome 9 at 15,997,402 bp
  • G to A, chromosome 9 at 21,281,640 bp
  • CGAGGAGGAGGAGGAGG to CGAGGAGGAGGAGG, chromosome 9 at 21,700,943 bp
  • A to G, chromosome 11 at 104,343,538 bp
  • T to C, chromosome 12 at 98,440,685 bp
  • C to T, chromosome 13 at 100,481,054 bp
  • G to A, chromosome 14 at 34,070,450 bp
  • T to A, chromosome 14 at 50,765,958 bp
  • T to C, chromosome 15 at 78,973,898 bp
  • A to G, chromosome 15 at 79,713,447 bp
  • T to C, chromosome 17 at 30,736,155 bp
  • A to T, chromosome 17 at 46,686,404 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0665 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038850-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.