Strain Name:
C57BL/6J-MtgxR1245Btlr/Mmmh
Stock Number:
039312-MU
Citation ID:
RRID:MMRRC_039312-MU
Other Names:
R1245 (G1), C57BL/6J-MtgxR1245Btlr
Major Collection:

Gene Information

Ulk1
Name: unc-51 like kinase 1
Synonyms: Unc51.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22241
Homologene: 2640
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232341
Homologene: 14253
Psma2
Name: proteasome subunit alpha 2
Synonyms: Lmpc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19166
VEGA: 13
HGNC: HGNC:9531
Homologene: 2081
Myc
Name: myelocytomatosis oncogene
Synonyms: c-myc, Nird, Niard, Myc2, bHLHe39
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17869
HGNC: HGNC:7553
Homologene: 31092
Adcy1
Name: adenylate cyclase 1
Synonyms: AC1, I-AC, D11Bwg1392e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 432530
HGNC: HGNC:232
Homologene: 41419
Nmnat2
Name: nicotinamide nucleotide adenylyltransferase 2
Synonyms: PNAT1, D030041I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226518
Homologene: 75037
Enpp3
Name: ectonucleotide pyrophosphatase/phosphodiesterase 3
Synonyms: CD203c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 209558
VEGA: 10
HGNC: HGNC:3358
Homologene: 3683
Ibtk
Name: inhibitor of Bruton agammaglobulinemia tyrosine kinase
Synonyms: 5430411K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 108837
Homologene: 34661
Cblb
Name: Casitas B-lineage lymphoma b
Synonyms: Cbl-b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208650
VEGA: 16
HGNC: HGNC:1542
Homologene: 15856
Zfp653
Name: zinc finger protein 653
Synonyms: E430039K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319601
VEGA: 9
Homologene: 16346
Cd22
Name: CD22 antigen
Synonyms: Lyb-8, Lyb8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12483
HGNC: HGNC:1643
Homologene: 31052
Sppl2a
Name: signal peptide peptidase like 2A
Synonyms: C130089K23Rik, 2010106G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66552
Homologene: 36411
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: 4833413O10Rik, D430029O09Rik, 1110021D17Rik, Polydom
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 64817
Homologene: 23386
Zfyve9
Name: zinc finger, FYVE domain containing 9
Synonyms: Madhip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230597
HGNC: HGNC:6775
Homologene: 3527
Slc35e1
Name: solute carrier family 35, member E1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270066
Homologene: 49075
Vmn2r6
Name: vomeronasal 2, receptor 6
Synonyms: EG620718, EG667069
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 667069
Homologene: 129754
Ltbp1
Name: latent transforming growth factor beta binding protein 1
Synonyms: LTBP-1, 9430031G15Rik, b2b1000Clo, Ltbp1L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268977
HGNC: HGNC:6714
Homologene: 522
Unc80
Name: unc-80, NALCN activator
Synonyms: C230061B10Rik, C030018G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329178
Homologene: 122243
Nckap1l
Name: NCK associated protein 1 like
Synonyms: 4930568P13Rik, Hem1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105855
VEGA: 15
HGNC: HGNC:4862
Homologene: 3901
Kctd7
Name: potassium channel tetramerisation domain containing 7
Synonyms: 9430010P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 212919
Homologene: 17687
Col6a6
Name: collagen, type VI, alpha 6
Synonyms: E330026B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 245026
Homologene: 18260
Ahnak
Name: AHNAK nucleoprotein (desmoyokin)
Synonyms: DY6, 2310047C17Rik, 1110004P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Shank2
Name: SH3 and multiple ankyrin repeat domains 2
Synonyms: ProSAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 210274
Homologene: 105965
Gm5828
Name: predicted gene 5828
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545306
Zfp948
Name: zinc finger protein 948
Synonyms: BC049807
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381066
VEGA: 17
Smg8
Name: SMG8 nonsense mediated mRNA decay factor
Synonyms: 1200011M11Rik, smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74133
Homologene: 32393
Ppp2r3a
Name: protein phosphatase 2, regulatory subunit B'', alpha
Synonyms: 3222402P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235542
HGNC: HGNC:9307
Homologene: 20595
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 628185
Homologene: 86604
Zscan29
Name: zinc finger SCAN domains 29
Synonyms: Zfp690
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99334
Homologene: 17586
Lrfn2
Name: leucine rich repeat and fibronectin type III domain containing 2
Synonyms: 5730420O05Rik, SALM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70530
Homologene: 19182
Olfr1122
Name: olfactory receptor 1122
Synonyms: GA_x6K02T2Q125-48880078-48881058, MOR264-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 259033
Homologene: 73977
AC147806.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Gpr137c
Name: G protein-coupled receptor 137C
Synonyms: LOC380893, TM7SF1L2, 6330416L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 70713
Homologene: 28613
Dlg2
Name: discs large MAGUK scaffold protein 2
Synonyms: PSD93, Chapsyn-110, B330007M19Rik, A330103J02Rik, LOC382816, Dlgh2, B230218P12Rik, Gm21505
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23859
HGNC: HGNC:2901
Homologene: 1046
Lmcd1
Name: LIM and cysteine-rich domains 1
Synonyms: dyxin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 30937
HGNC: HGNC:6633
Homologene: 22823
Ncs1
Name: neuronal calcium sensor 1
Synonyms: NCS-1, 9430075O15Rik, A730032G13Rik, Freq
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14299
HGNC: HGNC:3953
Homologene: 5719
Rspo4
Name: R-spondin 4
Synonyms: A930029K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228770
Homologene: 65290
Csf1r
Name: colony stimulating factor 1 receptor
Synonyms: CSF-1R, M-CSFR, CD115, Fim-2, Fms, Csfmr, Fim2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12978
HGNC: HGNC:2433
Homologene: 3817
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 16,769,129 bp
  • A to G, chromosome 1 at 66,555,095 bp
  • CAGAGAGA to CAGAGAGAGA, chromosome 1 at 85,577,224 bp
  • A to G, chromosome 1 at 153,112,203 bp
  • A to G, chromosome 2 at 31,284,693 bp
  • T to A, chromosome 2 at 76,945,700 bp
  • T to A, chromosome 2 at 87,388,209 bp
  • T to C, chromosome 2 at 121,166,503 bp
  • A to T, chromosome 2 at 126,913,521 bp
  • G to A, chromosome 2 at 151,867,926 bp
  • A to G, chromosome 3 at 64,556,790 bp
  • A to G, chromosome 4 at 58,066,427 bp
  • A to G, chromosome 4 at 108,693,311 bp
  • A to G, chromosome 5 at 110,789,340 bp
  • A to G, chromosome 5 at 130,148,217 bp
  • T to A, chromosome 6 at 112,315,712 bp
  • C to T, chromosome 6 at 119,948,457 bp
  • A to G, chromosome 7 at 30,869,883 bp
  • T to C, chromosome 7 at 92,442,595 bp
  • G to A, chromosome 7 at 144,411,720 bp
  • T to C, chromosome 8 at 72,492,571 bp
  • A to T, chromosome 9 at 22,056,422 bp
  • A to G, chromosome 9 at 85,720,742 bp
  • G to A, chromosome 9 at 101,194,394 bp
  • G to A, chromosome 9 at 105,748,910 bp
  • G to A, chromosome 10 at 24,784,953 bp
  • T to A, chromosome 11 at 7,169,410 bp
  • T to C, chromosome 11 at 87,083,610 bp
  • A to G, chromosome 13 at 14,613,291 bp
  • T to C, chromosome 14 at 45,279,065 bp
  • A to G, chromosome 15 at 61,987,897 bp
  • A to G, chromosome 15 at 103,455,925 bp
  • C to A, chromosome 16 at 52,047,187 bp
  • T to C, chromosome 17 at 21,586,842 bp
  • A to G, chromosome 17 at 22,603,247 bp
  • A to T, chromosome 17 at 49,096,249 bp
  • C to T, chromosome 17 at 75,327,194 bp
  • C to A, chromosome 18 at 61,114,812 bp
  • A to G, chromosome 19 at 9,004,169 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1245 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039312-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.