Strain Name:
C57BL/6J-MtgxR1344Btlr/Mmmh
Stock Number:
039409-MU
Citation ID:
RRID:MMRRC_039409-MU
Other Names:
R1344 (G1), C57BL/6J-MtgxR1344Btlr
Major Collection:

Gene Information

Gene Symbol: Setd5 [MGI:1920145] (Mus musculus (mouse))
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Pde1c [MGI:108413] (Mus musculus (mouse))
Name: phosphodiesterase 1C
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Elf1 [MGI:107180] (Mus musculus (mouse))
Name: E74-like factor 1
Synonyms: Elf-1
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Macf1 [MGI:108559] (Mus musculus (mouse))
Name: microtubule-actin crosslinking factor 1
Synonyms: trabeculin alpha, Acf7, Aclp7
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Tet1 [MGI:1098693] (Mus musculus (mouse))
Name: tet methylcytosine dioxygenase 1
Synonyms: Cxxc6, BB001228, 2510010B09Rik, D10Ertd17e
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Gli2 [MGI:95728] (Mus musculus (mouse))
Name: GLI-Kruppel family member GLI2
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Dusp1 [MGI:105120] (Mus musculus (mouse))
Name: dual specificity phosphatase 1
Synonyms: Ptpn16, erp, mkp-1, MKP1, 3CH134
Chromosome: 17
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 53,647,236 bp
  • C to A, chromosome 1 at 90,142,731 bp
  • T to G, chromosome 1 at 118,841,936 bp
  • G to A, chromosome 1 at 131,021,492 bp
  • A to C, chromosome 1 at 155,328,697 bp
  • T to C, chromosome 1 at 170,961,081 bp
  • T to C, chromosome 1 at 191,985,301 bp
  • A to G, chromosome 2 at 76,735,411 bp
  • A to T, chromosome 2 at 90,853,015 bp
  • A to G, chromosome 2 at 130,600,290 bp
  • C to T, chromosome 2 at 174,345,214 bp
  • T to C, chromosome 2 at 181,237,596 bp
  • T to A, chromosome 3 at 34,058,012 bp
  • A to G, chromosome 3 at 90,238,738 bp
  • A to G, chromosome 3 at 95,470,399 bp
  • A to T, chromosome 3 at 101,018,775 bp
  • T to C, chromosome 3 at 130,007,423 bp
  • T to C, chromosome 4 at 40,179,008 bp
  • T to A, chromosome 4 at 43,028,197 bp
  • T to A, chromosome 4 at 81,308,319 bp
  • T to G, chromosome 4 at 123,433,453 bp
  • T to C, chromosome 4 at 134,359,241 bp
  • T to A, chromosome 5 at 25,953,109 bp
  • A to G, chromosome 5 at 90,464,202 bp
  • T to C, chromosome 5 at 114,418,575 bp
  • C to T, chromosome 5 at 120,914,896 bp
  • C to T, chromosome 5 at 125,025,446 bp
  • G to A, chromosome 5 at 149,588,098 bp
  • T to G, chromosome 6 at 52,179,151 bp
  • T to C, chromosome 6 at 56,361,767 bp
  • T to C, chromosome 6 at 65,597,429 bp
  • G to A, chromosome 6 at 71,262,167 bp
  • G to A, chromosome 6 at 106,344,870 bp
  • C to T, chromosome 6 at 112,477,177 bp
  • T to C, chromosome 6 at 113,137,943 bp
  • T to C, chromosome 6 at 120,876,327 bp
  • A to G, chromosome 6 at 141,985,618 bp
  • A to T, chromosome 7 at 34,353,557 bp
  • G to T, chromosome 7 at 46,274,615 bp
  • A to T, chromosome 7 at 46,938,010 bp
  • A to G, chromosome 7 at 98,716,830 bp
  • A to G, chromosome 7 at 119,857,935 bp
  • T to C, chromosome 7 at 140,691,799 bp
  • C to T, chromosome 7 at 140,968,350 bp
  • A to C, chromosome 8 at 70,108,169 bp
  • C to T, chromosome 8 at 88,318,893 bp
  • G to C, chromosome 8 at 110,838,303 bp
  • T to A, chromosome 9 at 40,074,472 bp
  • A to T, chromosome 9 at 78,672,550 bp
  • T to A, chromosome 9 at 79,699,555 bp
  • C to T, chromosome 9 at 92,259,304 bp
  • G to A, chromosome 9 at 119,130,017 bp
  • A to T, chromosome 9 at 121,978,386 bp
  • C to A, chromosome 10 at 8,298,190 bp
  • C to T, chromosome 10 at 62,814,521 bp
  • G to A, chromosome 10 at 79,685,300 bp
  • G to A, chromosome 10 at 95,994,999 bp
  • A to G, chromosome 11 at 4,409,005 bp
  • G to A, chromosome 11 at 29,545,002 bp
  • G to A, chromosome 11 at 54,909,596 bp
  • A to G, chromosome 11 at 67,092,332 bp
  • A to G, chromosome 11 at 69,648,884 bp
  • A to T, chromosome 11 at 74,054,212 bp
  • A to C, chromosome 11 at 95,869,355 bp
  • T to A, chromosome 11 at 98,241,785 bp
  • T to A, chromosome 11 at 101,285,740 bp
  • A to G, chromosome 12 at 3,493,790 bp
  • A to G, chromosome 12 at 80,692,885 bp
  • T to C, chromosome 12 at 111,627,837 bp
  • T to C, chromosome 13 at 54,550,479 bp
  • C to A, chromosome 13 at 109,950,387 bp
  • T to C, chromosome 13 at 112,690,180 bp
  • A to G, chromosome 14 at 37,068,556 bp
  • T to A, chromosome 14 at 50,680,858 bp
  • T to C, chromosome 14 at 74,804,269 bp
  • T to C, chromosome 14 at 79,560,775 bp
  • A to G, chromosome 14 at 111,680,389 bp
  • T to A, chromosome 15 at 11,286,804 bp
  • A to T, chromosome 15 at 36,265,266 bp
  • A to T, chromosome 15 at 82,405,905 bp
  • A to T, chromosome 15 at 97,718,973 bp
  • T to C, chromosome 16 at 90,769,466 bp
  • A to G, chromosome 17 at 26,508,319 bp
  • A to G, chromosome 17 at 27,894,577 bp
  • A to C, chromosome 17 at 31,328,241 bp
  • A to T, chromosome 17 at 65,859,148 bp
  • A to T, chromosome 18 at 12,199,739 bp
  • A to G, chromosome 18 at 20,981,231 bp
  • G to A, chromosome 18 at 37,295,657 bp
  • T to A, chromosome 18 at 63,021,254 bp
  • A to G, chromosome 19 at 47,130,077 bp
  • T to A, chromosome 19 at 47,671,505 bp
  • G to A, chromosome 19 at 57,371,162 bp
  • G to A, chromosome 19 at 57,935,705 bp
  • A to C, chromosome 19 at 58,238,417 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1344 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039409-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.