Strain Name:
C57BL/6J-MtgxR1471Btlr/Mmmh
Stock Number:
039524-MU
Citation ID:
RRID:MMRRC_039524-MU
Other Names:
R1471 (G1), C57BL/6J-MtgxR1471Btlr
Major Collection:

Gene Information

Gene Symbol: Fga [MGI:1316726] (Mus musculus (mouse))
Name: fibrinogen alpha chain
Synonyms: ENSMUSG00000059807, Fib
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: B4galt6 [MGI:1928380] (Mus musculus (mouse))
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Slc26a5 [MGI:1933154] (Mus musculus (mouse))
Name: solute carrier family 26, member 5
Synonyms: prestin, Pres
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Foxm1 [MGI:1347487] (Mus musculus (mouse))
Name: forkhead box M1
Synonyms: WIN, Fkh16, HFH-11B, Foxm1b, Mpm2, Trident
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Cd2ap [MGI:1330281] (Mus musculus (mouse))
Name: CD2-associated protein
Synonyms: METS-1, Mets1
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Col18a1 [MGI:88451] (Mus musculus (mouse))
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Nup210l [MGI:1924845] (Mus musculus (mouse))
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 7,147,745 bp
  • A to G, chromosome 1 at 15,842,970 bp
  • T to A, chromosome 1 at 52,709,557 bp
  • A to T, chromosome 1 at 74,611,155 bp
  • C to A, chromosome 1 at 131,021,373 bp
  • A to G, chromosome 1 at 157,470,721 bp
  • T to A, chromosome 1 at 173,730,063 bp
  • T to C, chromosome 2 at 3,491,608 bp
  • A to G, chromosome 2 at 79,287,032 bp
  • C to T, chromosome 2 at 86,922,578 bp
  • A to G, chromosome 2 at 87,388,518 bp
  • T to C, chromosome 2 at 87,635,670 bp
  • T to C, chromosome 2 at 111,484,006 bp
  • T to C, chromosome 2 at 113,693,094 bp
  • T to C, chromosome 2 at 145,932,316 bp
  • C to A, chromosome 2 at 155,942,254 bp
  • C to T, chromosome 2 at 157,457,187 bp
  • C to A, chromosome 2 at 180,994,190 bp
  • A to G, chromosome 2 at 181,797,111 bp
  • T to C, chromosome 3 at 83,028,618 bp
  • A to C, chromosome 3 at 90,170,562 bp
  • T to A, chromosome 3 at 129,915,815 bp
  • C to T, chromosome 4 at 32,639,386 bp
  • C to T, chromosome 4 at 41,120,281 bp
  • C to T, chromosome 4 at 101,890,338 bp
  • T to C, chromosome 4 at 128,787,200 bp
  • T to C, chromosome 4 at 136,658,951 bp
  • T to C, chromosome 4 at 143,964,953 bp
  • T to C, chromosome 5 at 14,680,427 bp
  • T to A, chromosome 5 at 21,816,964 bp
  • T to A, chromosome 5 at 63,936,085 bp
  • A to G, chromosome 5 at 66,284,229 bp
  • C to T, chromosome 5 at 86,660,496 bp
  • C to T, chromosome 5 at 87,092,071 bp
  • T to A, chromosome 5 at 90,503,682 bp
  • C to A, chromosome 5 at 97,975,340 bp
  • C to T, chromosome 5 at 115,563,344 bp
  • T to A, chromosome 5 at 121,786,374 bp
  • T to G, chromosome 6 at 23,969,171 bp
  • C to A, chromosome 6 at 27,363,309 bp
  • T to C, chromosome 6 at 47,514,886 bp
  • T to G, chromosome 6 at 90,344,171 bp
  • T to A, chromosome 6 at 101,151,512 bp
  • T to C, chromosome 6 at 126,930,109 bp
  • C to T, chromosome 6 at 128,373,874 bp
  • C to T, chromosome 6 at 128,674,729 bp
  • A to T, chromosome 7 at 8,377,883 bp
  • G to A, chromosome 7 at 15,626,950 bp
  • T to A, chromosome 7 at 16,177,571 bp
  • C to T, chromosome 7 at 45,448,250 bp
  • A to G, chromosome 7 at 68,003,837 bp
  • T to C, chromosome 7 at 83,598,290 bp
  • A to T, chromosome 7 at 131,391,767 bp
  • A to G, chromosome 7 at 141,647,909 bp
  • A to T, chromosome 7 at 141,843,234 bp
  • A to T, chromosome 8 at 26,067,946 bp
  • A to T, chromosome 8 at 83,268,969 bp
  • A to G, chromosome 8 at 85,086,845 bp
  • G to A, chromosome 9 at 78,654,587 bp
  • A to G, chromosome 9 at 85,703,645 bp
  • A to G, chromosome 9 at 114,591,551 bp
  • T to C, chromosome 9 at 119,942,641 bp
  • G to A, chromosome 10 at 34,298,111 bp
  • A to G, chromosome 10 at 75,694,792 bp
  • A to T, chromosome 10 at 76,186,528 bp
  • G to T, chromosome 10 at 77,096,206 bp
  • T to C, chromosome 10 at 80,260,858 bp
  • A to G, chromosome 10 at 88,445,763 bp
  • A to G, chromosome 10 at 99,108,674 bp
  • A to G, chromosome 10 at 115,239,353 bp
  • C to A, chromosome 11 at 23,488,862 bp
  • A to T, chromosome 11 at 23,615,222 bp
  • T to C, chromosome 11 at 70,056,093 bp
  • C to A, chromosome 11 at 83,512,821 bp
  • A to C, chromosome 11 at 89,055,494 bp
  • T to A, chromosome 11 at 106,046,747 bp
  • A to G, chromosome 11 at 114,959,788 bp
  • T to C, chromosome 11 at 116,049,409 bp
  • A to T, chromosome 12 at 16,711,774 bp
  • A to G, chromosome 12 at 53,141,496 bp
  • T to A, chromosome 12 at 113,544,393 bp
  • C to A, chromosome 13 at 60,853,522 bp
  • G to A, chromosome 13 at 61,196,380 bp
  • T to C, chromosome 13 at 81,543,364 bp
  • T to C, chromosome 13 at 115,101,463 bp
  • C to T, chromosome 14 at 31,228,045 bp
  • T to C, chromosome 14 at 57,945,397 bp
  • A to G, chromosome 14 at 69,693,097 bp
  • T to A, chromosome 15 at 85,817,133 bp
  • A to T, chromosome 15 at 90,956,419 bp
  • A to G, chromosome 16 at 36,753,791 bp
  • A to G, chromosome 16 at 88,825,627 bp
  • T to A, chromosome 17 at 23,478,473 bp
  • T to A, chromosome 17 at 23,820,796 bp
  • T to C, chromosome 17 at 24,680,072 bp
  • T to A, chromosome 17 at 33,553,138 bp
  • T to C, chromosome 17 at 42,820,597 bp
  • T to A, chromosome 17 at 53,399,112 bp
  • T to C, chromosome 17 at 66,379,148 bp
  • C to A, chromosome 17 at 69,388,502 bp
  • C to T, chromosome 18 at 20,745,353 bp
  • A to G, chromosome 18 at 22,516,354 bp
  • C to T, chromosome 18 at 62,596,204 bp
  • C to A, chromosome 18 at 77,781,269 bp
  • A to G, chromosome 19 at 6,854,023 bp
  • T to G, chromosome 19 at 9,012,932 bp
  • T to C, chromosome 19 at 17,568,324 bp
  • C to A, chromosome 19 at 25,201,036 bp
  • A to T, chromosome 19 at 41,924,718 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1471 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039524-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.