Strain Name:
C57BL/6J-MtgxR1512Btlr/Mmmh
Stock Number:
039559-MU
Citation ID:
RRID:MMRRC_039559-MU
Other Names:
R1512 (G1), C57BL/6J-MtgxR1512Btlr
Major Collection:

Gene Information

Gene Symbol: Meis1 [MGI:104717] (Mus musculus (mouse))
Name: Meis homeobox 1
Synonyms: C530044H18Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Emx2 [MGI:95388] (Mus musculus (mouse))
Name: empty spiracles homeobox 2
Synonyms: Pdo
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Kl [MGI:1101771] (Mus musculus (mouse))
Name: klotho
Synonyms: alpha-kl
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Gtf2h3 [MGI:1277143] (Mus musculus (mouse))
Name: general transcription factor IIH, polypeptide 3
Synonyms: BTF2, 5033417D07Rik, D5Ertd679e, 34kDa
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Prkdc [MGI:104779] (Mus musculus (mouse))
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNAPDcs, DNA-PK, slip, DOXNPH, XRCC7, DNA-PKcs, dxnph
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Ppp1r13b [MGI:1336199] (Mus musculus (mouse))
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Rps6ka1 [MGI:104558] (Mus musculus (mouse))
Name: ribosomal protein S6 kinase polypeptide 1
Synonyms: Rsk1
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 11,061,330 bp
  • A to G, chromosome 1 at 58,307,351 bp
  • T to C, chromosome 1 at 71,088,263 bp
  • T to A, chromosome 1 at 81,241,851 bp
  • T to C, chromosome 1 at 115,900,950 bp
  • G to T, chromosome 1 at 132,523,692 bp
  • A to G, chromosome 1 at 136,152,805 bp
  • A to T, chromosome 1 at 151,360,936 bp
  • G to C, chromosome 1 at 151,360,937 bp
  • T to C, chromosome 1 at 165,352,020 bp
  • T to C, chromosome 1 at 184,882,906 bp
  • G to A, chromosome 2 at 28,451,516 bp
  • A to C, chromosome 2 at 66,331,285 bp
  • G to T, chromosome 2 at 84,855,459 bp
  • A to T, chromosome 2 at 87,810,644 bp
  • A to C, chromosome 2 at 167,056,317 bp
  • T to C, chromosome 3 at 96,580,874 bp
  • A to G, chromosome 3 at 146,454,965 bp
  • C to A, chromosome 3 at 151,746,003 bp
  • A to G, chromosome 4 at 53,786,107 bp
  • T to C, chromosome 4 at 72,141,258 bp
  • A to G, chromosome 4 at 113,238,132 bp
  • A to G, chromosome 4 at 126,029,984 bp
  • C to T, chromosome 4 at 133,851,004 bp
  • G to A, chromosome 4 at 139,360,184 bp
  • A to G, chromosome 5 at 24,383,739 bp
  • C to A, chromosome 5 at 72,108,704 bp
  • T to A, chromosome 5 at 72,108,705 bp
  • T to A, chromosome 5 at 124,590,870 bp
  • T to C, chromosome 5 at 130,000,890 bp
  • A to T, chromosome 5 at 138,297,985 bp
  • G to T, chromosome 5 at 139,050,673 bp
  • A to G, chromosome 5 at 150,988,597 bp
  • C to A, chromosome 6 at 84,576,997 bp
  • A to G, chromosome 6 at 112,387,998 bp
  • A to T, chromosome 7 at 18,656,500 bp
  • A to G, chromosome 7 at 45,315,044 bp
  • T to A, chromosome 7 at 51,579,568 bp
  • C to A, chromosome 8 at 3,535,459 bp
  • T to C, chromosome 8 at 110,222,635 bp
  • A to G, chromosome 8 at 120,658,244 bp
  • C to T, chromosome 9 at 4,384,138 bp
  • A to G, chromosome 9 at 21,142,416 bp
  • A to G, chromosome 9 at 31,337,401 bp
  • T to A, chromosome 9 at 38,828,364 bp
  • T to C, chromosome 9 at 39,550,094 bp
  • T to C, chromosome 9 at 65,063,814 bp
  • G to A, chromosome 10 at 60,831,475 bp
  • C to T, chromosome 10 at 76,404,662 bp
  • A to G, chromosome 10 at 76,470,513 bp
  • A to G, chromosome 10 at 128,124,152 bp
  • T to A, chromosome 11 at 4,424,788 bp
  • T to G, chromosome 11 at 18,881,682 bp
  • A to G, chromosome 11 at 49,470,459 bp
  • T to C, chromosome 11 at 79,390,369 bp
  • T to A, chromosome 11 at 84,195,469 bp
  • C to T, chromosome 11 at 93,885,564 bp
  • C to T, chromosome 11 at 94,561,398 bp
  • T to A, chromosome 11 at 115,253,850 bp
  • C to T, chromosome 11 at 116,147,927 bp
  • A to T, chromosome 11 at 118,095,015 bp
  • A to G, chromosome 12 at 52,937,154 bp
  • T to A, chromosome 12 at 75,119,937 bp
  • A to G, chromosome 12 at 110,635,756 bp
  • T to C, chromosome 12 at 111,872,408 bp
  • G to C, chromosome 12 at 112,146,955 bp
  • A to T, chromosome 13 at 54,466,178 bp
  • G to T, chromosome 14 at 31,293,037 bp
  • A to G, chromosome 14 at 32,960,808 bp
  • A to T, chromosome 14 at 56,467,786 bp
  • A to G, chromosome 14 at 97,901,399 bp
  • T to C, chromosome 15 at 31,367,229 bp
  • A to T, chromosome 15 at 97,757,501 bp
  • A to T, chromosome 16 at 15,687,404 bp
  • A to G, chromosome 16 at 20,122,936 bp
  • G to T, chromosome 16 at 44,221,198 bp
  • A to T, chromosome 16 at 44,766,027 bp
  • A to T, chromosome 17 at 23,668,109 bp
  • G to A, chromosome 17 at 78,237,929 bp
  • A to T, chromosome 17 at 83,220,044 bp
  • T to C, chromosome 18 at 30,322,236 bp
  • A to G, chromosome 18 at 44,342,355 bp
  • A to G, chromosome 18 at 54,946,666 bp
  • C to T, chromosome 18 at 59,048,845 bp
  • T to G, chromosome 18 at 75,008,696 bp
  • G to A, chromosome 19 at 16,872,514 bp
  • A to T, chromosome 19 at 18,875,931 bp
  • A to G, chromosome 19 at 29,951,990 bp
  • T to C, chromosome 19 at 59,459,603 bp
  • G to A, chromosome X at 36,020,035 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1512 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039559-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.