Strain Name:
C57BL/6J-MtgxR1517Btlr/Mmmh
Stock Number:
039563-MU
Citation ID:
RRID:MMRRC_039563-MU
Other Names:
R1517 (G1), C57BL/6J-MtgxR1517Btlr
Major Collection:

Gene Information

Gene Symbol: Fga [MGI:1316726] (Mus musculus (mouse))
Name: fibrinogen alpha chain
Synonyms: ENSMUSG00000059807, Fib
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Gba [MGI:95665] (Mus musculus (mouse))
Name: glucosidase, beta, acid
Synonyms: glucocerebrosidase, betaGC, GCase, GC, GBA1
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Aldh7a1 [MGI:108186] (Mus musculus (mouse))
Name: aldehyde dehydrogenase family 7, member A1
Synonyms: Atq1, D18Wsu181e
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Sox10 [MGI:98358] (Mus musculus (mouse))
Name: SRY (sex determining region Y)-box 10
Synonyms: gt, Sox21
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Actn4 [MGI:1890773] (Mus musculus (mouse))
Name: actinin alpha 4
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Uck2 [MGI:1931744] (Mus musculus (mouse))
Name: uridine-cytidine kinase 2
Synonyms: Umpk, TSA903
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Gria4 [MGI:95811] (Mus musculus (mouse))
Name: glutamate receptor, ionotropic, AMPA4 (alpha 4)
Synonyms: spkw1, Glur4, Glur-4, Gluralpha4
Chromosome: 9
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 13,165,057 bp
  • G to T, chromosome 1 at 36,958,377 bp
  • T to C, chromosome 1 at 55,064,257 bp
  • T to C, chromosome 1 at 150,669,421 bp
  • T to A, chromosome 1 at 158,579,576 bp
  • T to C, chromosome 1 at 167,234,724 bp
  • G to T, chromosome 2 at 22,282,634 bp
  • A to T, chromosome 2 at 28,859,857 bp
  • C to T, chromosome 2 at 30,354,975 bp
  • C to A, chromosome 2 at 32,305,984 bp
  • GCCACCA to GCCA, chromosome 2 at 52,110,324 bp
  • G to A, chromosome 2 at 66,505,027 bp
  • T to A, chromosome 2 at 86,594,604 bp
  • T to A, chromosome 2 at 144,373,791 bp
  • A to T, chromosome 2 at 148,793,252 bp
  • A to G, chromosome 3 at 65,302,135 bp
  • T to C, chromosome 3 at 83,031,838 bp
  • A to T, chromosome 3 at 89,206,148 bp
  • A to T, chromosome 4 at 52,911,502 bp
  • C to A, chromosome 4 at 98,779,029 bp
  • A to G, chromosome 4 at 117,002,994 bp
  • A to T, chromosome 4 at 148,612,106 bp
  • T to A, chromosome 5 at 37,319,035 bp
  • T to C, chromosome 5 at 142,127,836 bp
  • C to T, chromosome 6 at 48,438,236 bp
  • T to A, chromosome 6 at 113,319,089 bp
  • T to A, chromosome 6 at 113,489,784 bp
  • T to A, chromosome 6 at 118,598,759 bp
  • A to C, chromosome 6 at 130,115,640 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • A to G, chromosome 7 at 127,260,508 bp
  • T to A, chromosome 8 at 21,997,358 bp
  • G to A, chromosome 8 at 95,743,213 bp
  • C to A, chromosome 8 at 105,395,376 bp
  • T to C, chromosome 8 at 129,061,681 bp
  • C to A, chromosome 9 at 4,793,865 bp
  • T to C, chromosome 9 at 39,669,720 bp
  • T to C, chromosome 10 at 39,838,443 bp
  • T to C, chromosome 10 at 78,050,587 bp
  • C to A, chromosome 10 at 80,154,123 bp
  • T to A, chromosome 11 at 11,487,754 bp
  • T to C, chromosome 11 at 46,751,235 bp
  • A to G, chromosome 11 at 54,117,645 bp
  • C to A, chromosome 11 at 63,070,490 bp
  • T to C, chromosome 11 at 69,684,738 bp
  • A to T, chromosome 11 at 73,710,644 bp
  • T to C, chromosome 11 at 80,353,398 bp
  • A to T, chromosome 11 at 102,466,325 bp
  • T to C, chromosome 11 at 109,971,814 bp
  • A to G, chromosome 12 at 65,133,813 bp
  • A to C, chromosome 12 at 76,329,262 bp
  • T to C, chromosome 12 at 79,252,151 bp
  • T to A, chromosome 12 at 116,059,605 bp
  • A to G, chromosome 13 at 27,385,099 bp
  • T to A, chromosome 13 at 75,098,047 bp
  • C to A, chromosome 13 at 93,662,339 bp
  • T to A, chromosome 15 at 9,065,001 bp
  • T to C, chromosome 15 at 10,399,003 bp
  • T to C, chromosome 15 at 35,300,667 bp
  • A to C, chromosome 15 at 39,035,236 bp
  • A to T, chromosome 15 at 68,110,430 bp
  • T to A, chromosome 15 at 79,159,178 bp
  • T to A, chromosome 15 at 81,407,456 bp
  • T to G, chromosome 15 at 82,558,136 bp
  • T to C, chromosome 15 at 99,238,209 bp
  • C to A, chromosome 16 at 25,889,253 bp
  • G to T, chromosome 16 at 62,885,512 bp
  • T to A, chromosome 17 at 36,872,454 bp
  • T to A, chromosome 17 at 57,223,110 bp
  • T to C, chromosome 17 at 74,408,847 bp
  • T to C, chromosome 18 at 10,626,913 bp
  • T to A, chromosome 18 at 23,832,838 bp
  • T to A, chromosome 18 at 37,478,098 bp
  • T to A, chromosome 18 at 37,489,620 bp
  • T to A, chromosome 18 at 56,532,061 bp
  • T to A, chromosome 18 at 60,300,898 bp
  • T to G, chromosome 18 at 61,690,245 bp
  • T to C, chromosome 18 at 62,178,800 bp
  • T to C, chromosome 18 at 89,113,350 bp
  • A to G, chromosome 19 at 15,959,663 bp
  • A to C, chromosome 19 at 37,698,860 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1517 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039563-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.