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Strain Name:
C57BL/6J-MtgxR1543Btlr/Mmmh
Stock Number:
039582-MU
Citation ID:
RRID:MMRRC_039582-MU
Other Names:
R1543 (G1), C57BL/6J-MtgxR1543Btlr
Major Collection:

Strain Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Acadm
Name: acyl-Coenzyme A dehydrogenase, medium chain
Synonyms: MCAD
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11364
HGNC: HGNC:89
Homologene: 3
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Slc17a5
Name: solute carrier family 17 (anion/sugar transporter), member 5
Synonyms: 4631416G20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235504
Homologene: 56571
Cemip2
Name: cell migration inducing hyaluronidase 2
Synonyms: 3110012M15Rik, Tmem2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 83921
VEGA: 19
Homologene: 75008
Phf14
Name: PHD finger protein 14
Synonyms: 1110001C23Rik, 4932409F11Rik, 5730446A07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 75725
Homologene: 8775
Mbtps1
Name: membrane-bound transcription factor peptidase, site 1
Synonyms: subtilisin/kexin isozyme-1, SKI-1, S1P, 0610038M03Rik, site-1 protease
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56453
Homologene: 2808
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 25,488,088 bp
  • A to G, chromosome 1 at 54,493,418 bp
  • C to A, chromosome 1 at 60,376,194 bp
  • T to A, chromosome 1 at 75,192,516 bp
  • A to G, chromosome 1 at 75,421,951 bp
  • A to G, chromosome 1 at 97,740,882 bp
  • T to A, chromosome 1 at 125,872,424 bp
  • A to T, chromosome 2 at 36,813,057 bp
  • C to A, chromosome 2 at 37,005,127 bp
  • A to T, chromosome 2 at 61,785,530 bp
  • C to T, chromosome 2 at 67,508,039 bp
  • G to A, chromosome 2 at 69,500,730 bp
  • A to T, chromosome 2 at 82,981,587 bp
  • A to G, chromosome 2 at 111,537,797 bp
  • A to T, chromosome 2 at 125,184,857 bp
  • A to G, chromosome 2 at 160,895,390 bp
  • A to T, chromosome 3 at 53,572,455 bp
  • G to A, chromosome 3 at 64,089,573 bp
  • G to A, chromosome 3 at 87,713,017 bp
  • T to C, chromosome 3 at 97,639,014 bp
  • T to C, chromosome 3 at 103,778,164 bp
  • A to C, chromosome 3 at 148,859,273 bp
  • T to G, chromosome 3 at 153,844,511 bp
  • A to G, chromosome 3 at 153,929,572 bp
  • T to A, chromosome 4 at 24,591,084 bp
  • T to C, chromosome 4 at 143,397,038 bp
  • A to G, chromosome 5 at 7,975,902 bp
  • A to T, chromosome 5 at 16,266,718 bp
  • T to C, chromosome 5 at 23,546,968 bp
  • C to A, chromosome 5 at 35,603,008 bp
  • T to A, chromosome 5 at 62,606,155 bp
  • G to A, chromosome 5 at 65,224,690 bp
  • C to A, chromosome 5 at 101,844,081 bp
  • A to G, chromosome 5 at 115,151,006 bp
  • G to A, chromosome 6 at 11,987,683 bp
  • G to A, chromosome 6 at 40,968,204 bp
  • G to A, chromosome 6 at 42,215,603 bp
  • T to C, chromosome 6 at 128,890,985 bp
  • T to G, chromosome 7 at 29,083,537 bp
  • T to C, chromosome 7 at 46,016,504 bp
  • T to C, chromosome 7 at 67,678,995 bp
  • T to A, chromosome 7 at 78,902,622 bp
  • T to A, chromosome 7 at 103,187,214 bp
  • A to G, chromosome 7 at 141,324,147 bp
  • T to A, chromosome 8 at 69,096,287 bp
  • A to G, chromosome 8 at 70,792,311 bp
  • A to G, chromosome 8 at 119,542,069 bp
  • T to C, chromosome 8 at 123,388,587 bp
  • C to T, chromosome 8 at 125,670,872 bp
  • T to A, chromosome 9 at 37,803,947 bp
  • T to A, chromosome 9 at 45,150,429 bp
  • T to C, chromosome 9 at 59,603,877 bp
  • C to T, chromosome 9 at 62,425,841 bp
  • A to G, chromosome 9 at 78,560,800 bp
  • T to C, chromosome 9 at 107,570,187 bp
  • C to A, chromosome 9 at 108,812,372 bp
  • A to G, chromosome 9 at 111,468,054 bp
  • G to A, chromosome 10 at 26,078,620 bp
  • T to C, chromosome 10 at 43,021,724 bp
  • A to G, chromosome 10 at 51,481,421 bp
  • C to A, chromosome 10 at 107,412,069 bp
  • A to G, chromosome 11 at 8,901,200 bp
  • T to A, chromosome 11 at 76,452,319 bp
  • A to G, chromosome 11 at 78,275,174 bp
  • A to C, chromosome 11 at 109,974,674 bp
  • A to G, chromosome 11 at 120,720,018 bp
  • T to C, chromosome 12 at 116,231,784 bp
  • C to T, chromosome 13 at 47,068,521 bp
  • G to T, chromosome 13 at 59,702,242 bp
  • A to T, chromosome 13 at 105,112,369 bp
  • T to A, chromosome 14 at 24,144,448 bp
  • T to A, chromosome 14 at 68,521,922 bp
  • G to A, chromosome 15 at 73,025,967 bp
  • T to C, chromosome 15 at 76,372,209 bp
  • T to C, chromosome 15 at 78,159,712 bp
  • G to C, chromosome 15 at 89,324,079 bp
  • G to A, chromosome 15 at 97,809,529 bp
  • A to T, chromosome 17 at 28,362,671 bp
  • T to C, chromosome 17 at 50,935,532 bp
  • C to T, chromosome 17 at 57,522,374 bp
  • T to C, chromosome 17 at 74,558,107 bp
  • T to A, chromosome 18 at 20,594,211 bp
  • A to T, chromosome 19 at 4,602,223 bp
  • A to G, chromosome 19 at 6,938,540 bp
  • A to G, chromosome 19 at 8,880,753 bp
  • G to T, chromosome 19 at 21,812,573 bp
  • C to A, chromosome 19 at 39,643,264 bp
  • A to G, chromosome 19 at 52,991,676 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1543 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039582-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.