Strain Name:
C57BL/6J-MtgxR1550Btlr/Mmmh
Stock Number:
039589-MU
Citation ID:
RRID:MMRRC_039589-MU
Other Names:
R1550 (G1), C57BL/6J-MtgxR1550Btlr
Major Collection:

Gene Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, B130038B15Rik, OREBP, TonEBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Gli1
Name: GLI-Kruppel family member GLI1
Synonyms: Zfp-5, Zfp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14632
VEGA: 10
HGNC: HGNC:4317
Homologene: 3859
Itk
Name: IL2 inducible T cell kinase
Synonyms: Tsk, Emt, Tcsk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16428
HGNC: HGNC:6171
Homologene: 4051
Aldh7a1
Name: aldehyde dehydrogenase family 7, member A1
Synonyms: Atq1, D18Wsu181e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 110695
HGNC: HGNC:877
Homologene: 913
Zfp97
Name: zinc finger protein 97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22759
Homologene: 133884
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: D230004K18Rik, b2b1625.2Clo, Gp330, Megalin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Ptpra
Name: protein tyrosine phosphatase, receptor type, A
Synonyms: Ptpa, RPTPalpha, PTP[a], RPTRalpha, PTPalpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19262
HGNC: HGNC:9664
Homologene: 20621
Cyp11b2
Name: cytochrome P450, family 11, subfamily b, polypeptide 2
Synonyms: Cyp11b, aldosterone synthase, steroid-11-beta-hydroxylase, Cyp11b-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13072
Homologene: 128035
Tlr6
Name: toll-like receptor 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21899
Homologene: 21223
Mgll
Name: monoglyceride lipase
Synonyms: Magl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 23945
Homologene: 38298
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: ARHGAP10, 5530401C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71435
Homologene: 10822
Kdm2b
Name: lysine (K)-specific demethylase 2B
Synonyms: Cxxc2, Fbxl10, Jhdm1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 30841
Homologene: 13069
Sipa1l3
Name: signal-induced proliferation-associated 1 like 3
Synonyms: 2610511M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74206
Homologene: 77938
Herc2
Name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Synonyms: D15F32S1h, D7H15F37S1, jdf2, rjs, D7H15F32S1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 15204
HGNC: HGNC:4868
Homologene: 3430
Tet2
Name: tet methylcytosine dioxygenase 2
Synonyms: E130014J05Rik, Ayu17-449
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 214133
Homologene: 49498
Itpr1
Name: inositol 1,4,5-trisphosphate receptor 1
Synonyms: opt, Pcp1, InsP3R type I, wblo, P400, Ip3r, Pcp-1, Itpr-1, IP3R1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16438
HGNC: HGNC:6180
Homologene: 1673
Dhx40
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 40
Synonyms: DDX40, 2410016C14Rik, ARG147
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67487
Homologene: 57000
Eef2
Name: eukaryotic translation elongation factor 2
Synonyms: Ef-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 13629
VEGA: 10
HGNC: HGNC:3214
Homologene: 134867
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21819
Homologene: 2430
Htr1a
Name: 5-hydroxytryptamine (serotonin) receptor 1A
Synonyms: 5-HT1A receptor, Gpcr18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 15550
VEGA: 13
HGNC: HGNC:5286
Homologene: 20148
Jph3
Name: junctophilin 3
Synonyms: JP-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 57340
Homologene: 10762
Zfp236
Name: zinc finger protein 236
Synonyms: LOC240456
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 329002
Homologene: 7198
Mtmr4
Name: myotubularin related protein 4
Synonyms: ZFYVE11, FYVE-DSP2, FYVE zinc finger phosphatase, ESTM44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 170749
HGNC: HGNC:7452
Homologene: 3440
Gnl2
Name: guanine nucleotide binding protein-like 2 (nucleolar)
Synonyms: Ngp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230737
Homologene: 6858
Ddx11
Name: DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
Synonyms: KRG2, essa15a, CHL1, 4732462I11Rik, CHLR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 320209
VEGA: 17
HGNC: HGNC:2736
Homologene: 68973
Grin1
Name: glutamate receptor, ionotropic, NMDA1 (zeta 1)
Synonyms: Nmdar, NR1, M100174, GluRzeta1, NMDAR1, Rgsc174
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14810
HGNC: HGNC:4584
Homologene: 7187
Ddit4l
Name: DNA-damage-inducible transcript 4-like
Synonyms: 1700108M02Rik, RTP801L, REDD2, 1700037B15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 73284
Homologene: 12698
Sirpa
Name: signal-regulatory protein alpha
Synonyms: CD172a, SHPS-1, Bit, SIRP, Ptpns1, P84
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19261
HGNC: HGNC:9662
Homologene: 7246
Tnfrsf11b
Name: tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
Synonyms: OCIF, Opg, TR1, OPG, osteoclastogenesis inhibitory factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18383
VEGA: 15
Homologene: 1912
Dhx57
Name: DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106794
VEGA: 17
Homologene: 56267
Nlgn1
Name: neuroligin 1
Synonyms: 6330415N05Rik, Nlg1, NL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 192167
Homologene: 56690
Tet3
Name: tet methylcytosine dioxygenase 3
Synonyms: D230004J03Rik, B430006D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 194388
Homologene: 35360
Stab2
Name: stabilin 2
Synonyms: FEEL-2, STAB-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 192188
Homologene: 23022
Chrd
Name: chordin
Synonyms: Chd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12667
HGNC: HGNC:1949
Homologene: 2774
Dlgap2
Name: DLG associated protein 2
Synonyms: Sapap2, DAP2, SAP90/PSD-95-associated protein 2, PSD-95/SAP90-binding protein 2, 6430596N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244310
HGNC: HGNC:2906
Homologene: 3484
Slc4a5
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 5
Synonyms: C330016K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232156
Homologene: 31125
Abcc6
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 6
Synonyms: Mrp6, DCC, Dyscalc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 27421
HGNC: HGNC:57
Homologene: 55559
Acss1
Name: acyl-CoA synthetase short-chain family member 1
Synonyms: Acas2l, 1110032O15Rik, Acas2, AceCS2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68738
Homologene: 56037
Kprp
Name: keratinocyte expressed, proline-rich
Synonyms: 1110001M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 433619
Homologene: 54921
Fam38b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Pde4dip
Name: phosphodiesterase 4D interacting protein (myomegalin)
Synonyms: D130016K21Rik, 4732458A06Rik, D3Bwg1078e, 9430063L05Rik, Usmg4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 83679
Homologene: 66961
Slc13a2
Name: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2
Synonyms: Nadc1, mNaDC-1, sodium/dicarboxylate co-transporter
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20500
Homologene: 2965
Heg1
Name: heart development protein with EGF-like domains 1
Synonyms: 5530401I02Rik, 9530025L16Rik, 4632417D23Rik, LOC268884
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 77446
Homologene: 35276
Mybpc1
Name: myosin binding protein C, slow-type
Synonyms: 8030451F13Rik, Slow-type C-protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 109272
HGNC: HGNC:7549
Homologene: 1846
Prrt3
Name: proline-rich transmembrane protein 3
Synonyms: B230206N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 210673
Homologene: 52099
Ivns1abp
Name: influenza virus NS1A binding protein
Synonyms: NS1-BP, HSPC068, ND1, NS-1, Nd1-L, Nd1-S, 1700126I16Rik, 1190004M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 117198
Homologene: 31384
Arf5
Name: ADP-ribosylation factor 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 11844
HGNC: HGNC:658
Homologene: 129625
Mansc4
Name: MANSC domain containing 4
Synonyms: Gm5887
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 545893
Homologene: 86837
Gm4788
Name: predicted gene 4788
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 214403
Dsc3
Name: desmocollin 3
Synonyms: 5430426I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13507
VEGA: 18
HGNC: HGNC:3037
Homologene: 1462
Serpinb11
Name: serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 11
Synonyms: 2310046M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66957
Homologene: 41653
Fbxw24
Name: F-box and WD-40 domain protein 24
Synonyms: Gm5162
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382106
Homologene: 110776
Tkt
Name: transketolase
Synonyms: p68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21881
Homologene: 68154
Sema5a
Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Synonyms: semF, Semaf, M-Sema D, 9130201M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20356
VEGA: 15
Homologene: 2949
Nfasc
Name: neurofascin
Synonyms: D430023G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269116
Homologene: 24945
Kcnb2
Name: potassium voltage gated channel, Shab-related subfamily, member 2
Synonyms: 9630047L19Rik, Kv2.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98741
HGNC: HGNC:6232
Homologene: 31263
Vmn2r118
Name: vomeronasal 2, receptor 118
Synonyms: Vmn2r119, EG383258, EG668547
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 383258
Homologene: 129687
Cdh18
Name: cadherin 18
Synonyms: B230220E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320865
HGNC: HGNC:1757
Homologene: 55858
Gbp2b
Name: guanylate binding protein 2b
Synonyms: Gbp1, Gbp-1, Mpa1, Mpa-1, Mag-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14468
HGNC: HGNC:4184
Homologene: 137233
Zfp780b
Name: zinc finger protein 780B
Synonyms: B230208L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 338354
Homologene: 85969
Vps16
Name: VSP16 CORVET/HOPS core subunit
Synonyms: 1810074M16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 80743
Homologene: 7116
Gm7075
Name: predicted gene 7075
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 631906
VEGA: 10
Mill1
Name: MHC I like leukocyte 1
Synonyms: 5530400I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 266815
Homologene: 105329
Fam161a
Name: family with sequence similarity 161, member A
Synonyms: 4930430E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 73873
Homologene: 19131
Ubqln3
Name: ubiquilin 3
Synonyms: 4933400K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244178
Homologene: 9700
1700015F17Rik
Name: RIKEN cDNA 1700015F17 gene
Synonyms: LOC381716
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381716
Homologene: 136447
Lypd6b
Name: LY6/PLAUR domain containing 6B
Synonyms: 2310010M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71897
Homologene: 12419
Setbp1
Name: SET binding protein 1
Synonyms: Seb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240427
Homologene: 9192
Arrdc1
Name: arrestin domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 215705
Homologene: 34835
Olfr6
Name: olfactory receptor 6
Synonyms: GA_x6K02T2PBJ9-9337331-9336381, MOR103-16, M50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233670
Homologene: 28439
Cby3
Name: chibby family member 3
Synonyms: 1700121K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Homologene: 67734
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 15,713,012 bp
  • T to C, chromosome 1 at 107,379,688 bp
  • T to C, chromosome 1 at 132,608,503 bp
  • T to A, chromosome 1 at 139,739,652 bp
  • G to T, chromosome 1 at 151,361,491 bp
  • A to T, chromosome 2 at 20,881,765 bp
  • A to G, chromosome 2 at 24,926,339 bp
  • A to G, chromosome 2 at 25,305,131 bp
  • A to G, chromosome 2 at 49,943,603 bp
  • A to G, chromosome 2 at 69,502,661 bp
  • A to T, chromosome 2 at 129,630,041 bp
  • A to G, chromosome 2 at 130,440,340 bp
  • G to A, chromosome 2 at 130,541,393 bp
  • A to T, chromosome 2 at 150,642,795 bp
  • A to G, chromosome 3 at 25,912,644 bp
  • T to A, chromosome 3 at 92,824,726 bp
  • T to C, chromosome 3 at 97,719,704 bp
  • G to T, chromosome 3 at 133,469,519 bp
  • T to A, chromosome 3 at 137,624,275 bp
  • G to T, chromosome 3 at 142,606,830 bp
  • T to C, chromosome 4 at 125,044,234 bp
  • C to A, chromosome 5 at 5,452,019 bp
  • T to A, chromosome 5 at 64,953,411 bp
  • A to G, chromosome 5 at 122,881,057 bp
  • C to T, chromosome 6 at 28,426,153 bp
  • C to A, chromosome 6 at 83,271,057 bp
  • A to T, chromosome 6 at 83,386,028 bp
  • C to A, chromosome 6 at 88,813,889 bp
  • C to A, chromosome 6 at 108,389,352 bp
  • A to T, chromosome 6 at 113,495,507 bp
  • T to C, chromosome 6 at 147,075,638 bp
  • T to C, chromosome 7 at 18,255,881 bp
  • T to C, chromosome 7 at 27,964,857 bp
  • A to T, chromosome 7 at 29,383,203 bp
  • A to G, chromosome 7 at 46,005,244 bp
  • T to A, chromosome 7 at 56,135,658 bp
  • A to G, chromosome 7 at 56,137,880 bp
  • T to A, chromosome 7 at 104,141,546 bp
  • T to A, chromosome 7 at 106,956,028 bp
  • A to G, chromosome 8 at 14,822,499 bp
  • A to T, chromosome 8 at 107,370,573 bp
  • A to T, chromosome 8 at 121,784,859 bp
  • G to T, chromosome 9 at 109,607,044 bp
  • A to G, chromosome 10 at 63,421,648 bp
  • A to G, chromosome 10 at 81,180,847 bp
  • A to T, chromosome 10 at 86,878,926 bp
  • A to G, chromosome 10 at 88,533,581 bp
  • A to T, chromosome 10 at 127,338,516 bp
  • A to G, chromosome 11 at 23,020,470 bp
  • G to T, chromosome 11 at 46,389,326 bp
  • A to T, chromosome 11 at 50,359,486 bp
  • G to T, chromosome 11 at 78,403,164 bp
  • A to T, chromosome 11 at 86,776,739 bp
  • A to T, chromosome 11 at 87,613,516 bp
  • A to G, chromosome 13 at 105,445,280 bp
  • A to G, chromosome 14 at 30,565,568 bp
  • T to A, chromosome 15 at 23,436,548 bp
  • G to A, chromosome 15 at 32,618,849 bp
  • A to G, chromosome 15 at 54,254,058 bp
  • C to T, chromosome 15 at 66,693,430 bp
  • T to C, chromosome 15 at 74,853,593 bp
  • A to G, chromosome 16 at 20,734,682 bp
  • T to A, chromosome 16 at 33,735,553 bp
  • T to A, chromosome 17 at 17,145,206 bp
  • C to T, chromosome 17 at 55,608,083 bp
  • C to T, chromosome 17 at 66,138,220 bp
  • A to G, chromosome 17 at 80,278,386 bp
  • A to T, chromosome 18 at 19,983,531 bp
  • A to G, chromosome 18 at 56,550,382 bp
  • T to A, chromosome 18 at 63,050,523 bp
  • A to T, chromosome 18 at 78,858,592 bp
  • A to T, chromosome 18 at 82,674,424 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1550 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039589-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.