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Strain Name:
C57BL/6J-MtgxR1581Btlr/Mmmh
Stock Number:
039618-MU
Citation ID:
RRID:MMRRC_039618-MU
Other Names:
R1581 (G1), C57BL/6J-MtgxR1581Btlr
Major Collection:

Strain Information

Sphk2
Name: sphingosine kinase 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56632
Homologene: 32456
Cnnm2
Name: cyclin M2
Synonyms: Acdp2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 94219
VEGA: 19
HGNC: HGNC:103
Homologene: 9761
Pank4
Name: pantothenate kinase 4
Synonyms: D030031I12Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269614
Homologene: 41235
Sptbn1
Name: spectrin beta, non-erythrocytic 1
Synonyms: beta fodrin, Spnb-2, spectrin G, brain spectrin, elf3, elf1, 9930031C03Rik, non-erythrocytic, Spnb2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20742
Homologene: 2354
Lpp
Name: LIM domain containing preferred translocation partner in lipoma
Synonyms: B130055L10Rik, 9430020K16Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 210126
VEGA: 16
HGNC: HGNC:6679
Homologene: 4075
Camsap3
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: Nezha, 2310057J16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69697
Homologene: 18966
Sh3gl3
Name: SH3-domain GRB2-like 3
Synonyms: SH3P13, EEN-B2, endophilin III, Sh3d2c, Sh3d2c2, endophilin A3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20408
Homologene: 37723
Eed
Name: embryonic ectoderm development
Synonyms: l(7)5Rn, l7Rn5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13626
HGNC: HGNC:3188
Homologene: 2814
Arfgef1
Name: ADP ribosylation factor guanine nucleotide exchange factor 1
Synonyms: D730028O18Rik, ARFGEP1, P200, BIG1, D130059B05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 211673
Homologene: 4687
Nup214
Name: nucleoporin 214
Synonyms: D2H9S46E, CAN
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227720
HGNC: HGNC:8064
Homologene: 38008
Kcnk1
Name: potassium channel, subfamily K, member 1
Synonyms: TWIK-1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16525
HGNC: HGNC:6272
Homologene: 1691
Esr1
Name: estrogen receptor 1 (alpha)
Synonyms: ESR, ERalpha, ER[a], Nr3a1, ERa, Estr, Estra, ER-alpha
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13982
HGNC: HGNC:3467
Homologene: 47906
Chmp7
Name: charged multivesicular body protein 7
Synonyms: 4930596K11Rik, 6330407G04Rik, CHMP family, member 7
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105513
VEGA: 14
Homologene: 14613
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Wars1
Name: tryptophanyl-tRNA synthetase1
Synonyms: Wars
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 22375
Homologene: 3084
Tmem43
Name: transmembrane protein 43
Synonyms: 1200015A22Rik, LUMA
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74122
Homologene: 11532
Rnd2
Name: Rho family GTPase 2
Synonyms: Arhn, Rohn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11858
Homologene: 21123
Casc3
Name: exon junction complex subunit
Synonyms: Btz, Mln51
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192160
Homologene: 7208
Rfwd3
Name: ring finger and WD repeat domain 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234736
Homologene: 41230
Pradc1
Name: protease-associated domain containing 1
Synonyms: 1700040I03Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73327
Homologene: 13008
Fcrl1
Name: Fc receptor-like 1
Synonyms: IFGP1, BXMAS1-like, A230020G22Rik, Fcrh1, moFcRH1S, moFcRH1L, moFcRH1, mIFGP1, mBXMH1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229499
Homologene: 50472
Il6st
Name: interleukin 6 signal transducer
Synonyms: gp130, CD130, D13Ertd699e, 5133400A03Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16195
HGNC: HGNC:6021
Homologene: 1645
Eps15
Name: epidermal growth factor receptor pathway substrate 15
Synonyms: 2410112D09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13858
HGNC: HGNC:3419
Homologene: 128359
Septin9
Name: septin 9
Synonyms: SL3-3 integration site 1, Sint1, Msf, MSF1, PNUTL4, Sept9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 53860
HGNC: HGNC:7323
Homologene: 90949
Nr2e1
Name: nuclear receptor subfamily 2, group E, member 1
Synonyms: Nr2e1, Tlx, Mtll, tailless
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21907
HGNC: HGNC:7973
Homologene: 37750
Skor1
Name: SKI family transcriptional corepressor 1
Synonyms: C230094B15Rik, Corl1, Lbxcor1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 207667
Homologene: 18175
Ppp2r5c
Name: protein phosphatase 2, regulatory subunit B', gamma
Synonyms: Band 8A, 2610043M05Rik, 2700063L20Rik, D12Bwg0916e, B56/PP2A gamma
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 26931
VEGA: 12
HGNC: HGNC:9311
Homologene: 128665
Trpv5
Name: transient receptor potential cation channel, subfamily V, member 5
Synonyms: CaT2, ECaC1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 194352
HGNC: HGNC:3145
Homologene: 10520
Bicdl1
Name: BICD family like cargo adaptor 1
Synonyms: 2210403N09Rik, BICDR-1, Ccdc64
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75665
Homologene: 18013
Scd2
Name: stearoyl-Coenzyme A desaturase 2
Synonyms: Scd-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20250
VEGA: 19
Homologene: 136612
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy-3, hy3, 1700034M11Rik, 4930545D19Rik, hyrh
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244653
Homologene: 52118
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 26875
Homologene: 69111
Lrrc37a
Name: leucine rich repeat containing 37A
Synonyms: LOC237954
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237954
Homologene: 138824
Adgrb3
Name: adhesion G protein-coupled receptor B3
Synonyms: A830096D10Rik, Bai3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210933
HGNC: HGNC:945
Homologene: 1289
Nav3
Name: neuron navigator 3
Synonyms: Pomfil1p, POMFIL1, 4732483H20Rik, unc53H3, steerin 3, 9630020C08Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 260315
Homologene: 56688
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241516
Homologene: 110349
Zfp941
Name: zinc finger protein 941
Synonyms: BC066028
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 407812
Ttc39d
Name: tetratricopeptide repeat domain 39D
Synonyms: 4930560E09Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67737
Homologene: 65053
Ly75
Name: lymphocyte antigen 75
Synonyms: DEC-205, CD205
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17076
Homologene: 31085
Auh
Name: AU RNA binding protein/enoyl-coenzyme A hydratase
Synonyms: W91705
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 11992
HGNC: HGNC:890
Homologene: 1284
Vmn2r5
Name: vomeronasal 2, receptor 5
Synonyms: EG667060
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 667060
Gria1
Name: glutamate receptor, ionotropic, AMPA1 (alpha 1)
Synonyms: GluR1, GluR-A, Glr-1, Glur-1, Glur1, GluRA, Glr1, 2900051M01Rik, HIPA1, GluA1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14799
HGNC: HGNC:4571
Homologene: 20226
Padi6
Name: peptidyl arginine deiminase, type VI
Synonyms: ePAD, Padi5, Pad6
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242726
Homologene: 17695
Sipa1l2
Name: signal-induced proliferation-associated 1 like 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244668
Homologene: 18956
Fam118a
Name: family with sequence similarity 118, member A
Synonyms: C230014M12Rik, 3110048E14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 73225
VEGA: 15
HGNC: HGNC:1313
Homologene: 9911
Zfp27
Name: zinc finger protein 27
Synonyms: mkr-4, Zfp-27
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22689
Homologene: 81823
Or1r1
Name: olfactory receptor family 1 subfamily JRmember 1
Synonyms: GA_x6K02T2P1NL-4141430-4140486, MOR157-1, Olfr398
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258705
HGNC: HGNC:8226
Homologene: 17355
Luzp2
Name: leucine zipper protein 2
Synonyms: 9330154K17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233271
Homologene: 45618
Foxred2
Name: FAD-dependent oxidoreductase domain containing 2
Synonyms: LOC239554, A430097D04Rik, D15Bwg0759e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239554
Homologene: 11800
Etnppl
Name: ethanolamine phosphate phospholyase
Synonyms: 1300019H02Rik, Agxt2l1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71760
Homologene: 69440
Tmem67
Name: transmembrane protein 67
Synonyms: 5330408M12Rik, b2b1291.1Clo, b2b1163.1Clo
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329795
Homologene: 71886
Plppr4
Name: phospholipid phosphatase related 4
Synonyms: D3Bwg0562e, Lppr4, PRG-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229791
Homologene: 8895
H6pd
Name: hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
Synonyms: Gpd-1, Gpd1, G6pd1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100198
HGNC: HGNC:4795
Homologene: 48275
Hyou1
Name: hypoxia up-regulated 1
Synonyms: CBP-140, Orp150, 140 kDa, Grp170, Cab140
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12282
Homologene: 4658
Abcd2
Name: ATP-binding cassette, sub-family D member 2
Synonyms: ALDR, ALDL1, adrenoleukodystrophy related, ABC39
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 26874
VEGA: 15
HGNC: HGNC:66
Homologene: 55873
Zeb2
Name: zinc finger E-box binding homeobox 2
Synonyms: SIP1, Zfx1b, 9130203F04Rik, D130016B08Rik, Zfhx1b
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 24136
Homologene: 8868
Dus3l
Name: dihydrouridine synthase 3 like
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224907
VEGA: 17
Homologene: 6533
Cibar1
Name: CBY1 interacting BAR domain containing 1
Synonyms: 6720467C03Rik, Fam92a
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68099
Homologene: 12234
Brsk1
Name: BR serine/threonine kinase 1
Synonyms: LOC381979, SAD-B
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381979
Homologene: 57169
Ttll1
Name: tubulin tyrosine ligase-like 1
Synonyms: 6330444E16Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 319953
HGNC: HGNC:1312
Homologene: 8174
Fancg
Name: Fanconi anemia, complementation group G
Synonyms: Xrcc9
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 60534
HGNC: HGNC:3588
Homologene: 3402
Klk1b22
Name: kallikrein 1-related peptidase b22
Synonyms: mGk-22, Egfbp-1, Egfbp1, Klk22
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13646
Homologene: 68141
Upp2
Name: uridine phosphorylase 2
Synonyms: UDRPASE2, UPASE2, UP2, 1700124F02Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76654
Homologene: 12654
Klrh1
Name: killer cell lectin-like receptor subfamily H, member 1
Synonyms: LOC232415, Gm156
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232415
Homologene: 16395
Setbp1
Name: SET binding protein 1
Synonyms: Seb
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240427
Homologene: 9192
Bmyc
Name: brain expressed myelocytomatosis oncogene
Synonyms: 2900002K07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 107771
HGNC: HGNC:7553
Homologene: 11343
Tfec
Name: transcription factor EC
Synonyms: TFEC, bHLHe34, Tcfec
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21426
Homologene: 32148
Mesp2
Name: mesoderm posterior 2
Synonyms: bHLHc6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17293
Homologene: 7420
Rtbdn
Name: retbindin
Synonyms: A330096I21Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234542
Homologene: 12865
AC164627.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Kdelr3
Name: KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105785
VEGA: 15
HGNC: HGNC:6306
Homologene: 68533
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 10,199,878 bp
  • A to G, chromosome 1 at 25,094,072 bp
  • T to C, chromosome 2 at 25,707,334 bp
  • C to T, chromosome 2 at 32,034,466 bp
  • A to T, chromosome 2 at 44,997,000 bp
  • A to C, chromosome 2 at 58,774,165 bp
  • C to T, chromosome 2 at 60,327,893 bp
  • A to T, chromosome 2 at 82,986,282 bp
  • A to G, chromosome 3 at 64,491,219 bp
  • G to A, chromosome 3 at 87,385,723 bp
  • A to G, chromosome 3 at 117,328,266 bp
  • A to G, chromosome 3 at 130,628,744 bp
  • C to A, chromosome 4 at 12,047,814 bp
  • T to A, chromosome 4 at 12,155,745 bp
  • G to A, chromosome 4 at 43,007,039 bp
  • T to A, chromosome 4 at 109,363,186 bp
  • T to C, chromosome 4 at 140,735,836 bp
  • T to A, chromosome 4 at 149,982,514 bp
  • C to T, chromosome 4 at 154,974,651 bp
  • T to G, chromosome 5 at 14,521,282 bp
  • G to T, chromosome 5 at 115,651,267 bp
  • A to T, chromosome 6 at 16,844,244 bp
  • T to C, chromosome 6 at 41,653,140 bp
  • G to A, chromosome 6 at 85,448,586 bp
  • T to A, chromosome 6 at 91,478,735 bp
  • T to C, chromosome 6 at 129,775,833 bp
  • C to T, chromosome 7 at 4,707,957 bp
  • T to C, chromosome 7 at 28,898,646 bp
  • T to A, chromosome 7 at 29,896,124 bp
  • C to T, chromosome 7 at 41,043,831 bp
  • A to G, chromosome 7 at 44,115,975 bp
  • A to G, chromosome 7 at 45,713,496 bp
  • T to A, chromosome 7 at 55,249,490 bp
  • T to A, chromosome 7 at 79,812,541 bp
  • T to A, chromosome 7 at 82,268,357 bp
  • T to C, chromosome 7 at 89,980,468 bp
  • A to T, chromosome 7 at 140,812,120 bp
  • C to T, chromosome 8 at 3,604,708 bp
  • A to G, chromosome 8 at 84,955,066 bp
  • A to T, chromosome 8 at 110,410,460 bp
  • C to T, chromosome 8 at 111,288,242 bp
  • T to A, chromosome 8 at 125,491,617 bp
  • T to C, chromosome 8 at 125,995,539 bp
  • C to T, chromosome 9 at 44,388,870 bp
  • T to C, chromosome 9 at 63,146,223 bp
  • T to C, chromosome 10 at 4,997,905 bp
  • T to C, chromosome 10 at 42,567,968 bp
  • T to C, chromosome 10 at 100,306,612 bp
  • T to C, chromosome 10 at 109,823,428 bp
  • A to G, chromosome 11 at 30,129,671 bp
  • T to C, chromosome 11 at 57,237,010 bp
  • A to T, chromosome 11 at 73,984,521 bp
  • C to T, chromosome 11 at 98,822,818 bp
  • A to G, chromosome 11 at 101,471,196 bp
  • G to A, chromosome 11 at 103,457,017 bp
  • A to G, chromosome 11 at 117,290,595 bp
  • C to A, chromosome 12 at 108,875,709 bp
  • A to G, chromosome 12 at 110,552,897 bp
  • A to G, chromosome 13 at 11,794,563 bp
  • G to A, chromosome 13 at 52,835,496 bp
  • A to G, chromosome 13 at 112,481,541 bp
  • G to T, chromosome 14 at 61,213,679 bp
  • C to T, chromosome 14 at 69,719,450 bp
  • A to G, chromosome 15 at 77,955,761 bp
  • T to C, chromosome 15 at 79,522,913 bp
  • C to T, chromosome 15 at 83,496,277 bp
  • T to C, chromosome 15 at 85,050,679 bp
  • T to C, chromosome 15 at 91,179,144 bp
  • C to A, chromosome 16 at 24,681,841 bp
  • T to A, chromosome 17 at 56,768,509 bp
  • A to C, chromosome 17 at 80,216,484 bp
  • A to G, chromosome 18 at 78,783,250 bp
  • A to G, chromosome 19 at 44,298,099 bp
  • A to G, chromosome 19 at 46,763,123 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1581 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039618-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.