Strain Name:
C57BL/6J-MtgxR1654Btlr/Mmmh
Stock Number:
039690-MU
Citation ID:
RRID:MMRRC_039690-MU
Other Names:
R1654 (G1), C57BL/6J-MtgxR1654Btlr
Major Collection:

Gene Information

Gene Symbol: Epha4 [MGI:98277] (Mus musculus (mouse))
Name: Eph receptor A4
Synonyms: Sek, Hek8, Tyro1, Cek8, Sek1, 2900005C20Rik, rb
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Vcan [MGI:102889] (Mus musculus (mouse))
Name: versican
Synonyms: DPEAAE, heart defect, Cspg2, PG-M, hdf, 5430420N07Rik
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Trdmt1 [MGI:1274787] (Mus musculus (mouse))
Name: tRNA aspartic acid methyltransferase 1
Synonyms: Rnmt2, Dnmt2
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Ptprk [MGI:103310] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type, K
Synonyms: PTPk, RPTPkappa
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Pkp4 [MGI:109281] (Mus musculus (mouse))
Name: plakophilin 4
Synonyms: p0071, Armrp, 5031422I09Rik, 9430019K17Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Dnmt1 [MGI:94912] (Mus musculus (mouse))
Name: DNA methyltransferase (cytosine-5) 1
Synonyms: MTase, MommeD2, Dnmt1o, Cxxc9
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Arhgef12 [MGI:1916882] (Mus musculus (mouse))
Name: Rho guanine nucleotide exchange factor (GEF) 12
Synonyms: 2310014B11Rik, LARG
Chromosome: 9
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 58,647,118 bp
  • T to A, chromosome 1 at 77,374,768 bp
  • A to T, chromosome 1 at 111,862,512 bp
  • C to T, chromosome 1 at 131,697,073 bp
  • T to A, chromosome 1 at 169,745,311 bp
  • C to T, chromosome 1 at 171,884,606 bp
  • T to A, chromosome 1 at 183,681,945 bp
  • T to C, chromosome 1 at 191,163,481 bp
  • C to T, chromosome 2 at 13,516,730 bp
  • T to A, chromosome 2 at 25,680,775 bp
  • T to C, chromosome 2 at 29,950,587 bp
  • T to C, chromosome 2 at 32,619,509 bp
  • A to T, chromosome 2 at 59,337,619 bp
  • G to T, chromosome 2 at 120,703,722 bp
  • A to G, chromosome 2 at 121,164,779 bp
  • G to A, chromosome 2 at 130,393,546 bp
  • C to A, chromosome 2 at 132,815,835 bp
  • A to G, chromosome 2 at 140,177,300 bp
  • C to T, chromosome 2 at 170,349,246 bp
  • A to G, chromosome 3 at 36,638,406 bp
  • T to C, chromosome 3 at 94,423,680 bp
  • G to A, chromosome 4 at 3,789,912 bp
  • G to T, chromosome 4 at 9,453,315 bp
  • A to G, chromosome 4 at 53,761,220 bp
  • A to G, chromosome 4 at 106,634,211 bp
  • T to C, chromosome 4 at 116,316,550 bp
  • G to T, chromosome 5 at 26,261,099 bp
  • C to T, chromosome 5 at 36,483,795 bp
  • A to T, chromosome 5 at 67,611,059 bp
  • T to C, chromosome 5 at 149,422,135 bp
  • T to C, chromosome 6 at 40,757,487 bp
  • T to C, chromosome 6 at 95,655,551 bp
  • G to T, chromosome 6 at 97,118,963 bp
  • A to G, chromosome 6 at 121,336,926 bp
  • G to T, chromosome 6 at 124,317,581 bp
  • G to A, chromosome 6 at 124,493,910 bp
  • C to A, chromosome 7 at 3,235,144 bp
  • A to G, chromosome 7 at 17,759,616 bp
  • T to C, chromosome 7 at 25,338,486 bp
  • T to A, chromosome 7 at 86,811,915 bp
  • A to T, chromosome 7 at 104,688,213 bp
  • A to T, chromosome 7 at 116,368,848 bp
  • A to T, chromosome 7 at 119,926,449 bp
  • T to A, chromosome 7 at 135,653,928 bp
  • T to A, chromosome 7 at 141,102,418 bp
  • T to A, chromosome 7 at 141,278,854 bp
  • A to T, chromosome 8 at 61,361,745 bp
  • A to T, chromosome 8 at 64,117,903 bp
  • T to G, chromosome 8 at 86,631,450 bp
  • A to T, chromosome 8 at 125,148,465 bp
  • G to T, chromosome 9 at 20,936,574 bp
  • A to T, chromosome 9 at 21,804,843 bp
  • T to C, chromosome 9 at 42,997,660 bp
  • T to A, chromosome 9 at 67,951,687 bp
  • T to C, chromosome 9 at 102,586,913 bp
  • C to T, chromosome 10 at 18,625,148 bp
  • A to G, chromosome 10 at 20,137,018 bp
  • G to A, chromosome 10 at 28,383,647 bp
  • A to T, chromosome 10 at 29,146,935 bp
  • C to T, chromosome 10 at 80,301,842 bp
  • T to C, chromosome 10 at 109,853,123 bp
  • T to A, chromosome 10 at 115,184,807 bp
  • T to C, chromosome 10 at 116,188,363 bp
  • T to G, chromosome 10 at 118,150,780 bp
  • G to A, chromosome 11 at 49,430,502 bp
  • T to C, chromosome 11 at 59,543,123 bp
  • T to A, chromosome 11 at 60,947,194 bp
  • C to A, chromosome 11 at 69,779,169 bp
  • T to G, chromosome 11 at 71,181,298 bp
  • C to T, chromosome 11 at 109,797,399 bp
  • C to T, chromosome 11 at 115,260,853 bp
  • T to C, chromosome 11 at 116,358,715 bp
  • C to A, chromosome 12 at 59,108,833 bp
  • T to C, chromosome 12 at 76,101,094 bp
  • A to G, chromosome 13 at 46,848,391 bp
  • A to T, chromosome 13 at 54,681,779 bp
  • T to C, chromosome 13 at 89,661,946 bp
  • A to C, chromosome 14 at 50,963,383 bp
  • T to A, chromosome 14 at 77,611,374 bp
  • G to T, chromosome 15 at 5,098,732 bp
  • G to A, chromosome 15 at 9,634,652 bp
  • C to A, chromosome 15 at 80,389,210 bp
  • T to C, chromosome 15 at 101,351,709 bp
  • T to G, chromosome 16 at 33,975,738 bp
  • T to C, chromosome 16 at 59,119,689 bp
  • C to A, chromosome 16 at 93,894,903 bp
  • A to T, chromosome 17 at 30,987,968 bp
  • C to T, chromosome 17 at 56,808,263 bp
  • T to C, chromosome 18 at 20,046,246 bp
  • A to G, chromosome 18 at 32,863,059 bp
  • A to T, chromosome 18 at 34,611,226 bp
  • A to T, chromosome 18 at 37,436,701 bp
  • A to T, chromosome 18 at 59,176,416 bp
  • C to G, chromosome 18 at 64,512,049 bp
  • A to G, chromosome 18 at 73,930,939 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1654 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039690-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.